Team:Evry Paris-Saclay/Parts

Title




During this project, our team designed the following basic parts:


Coding sequences

Part Number Part Name
BBa_K2983003 & BBa_K2983103 Ura3
BBa_K2983060 RedStar2
BBa_K2983061 FadX of Punica granatum
BBa_K2983062 FadX of Trichosanthes kirilowii
BBa_K2983063 putative FadX of Jacaranda mimosifolia

Promoters

Part Number Part Name
BBa_K2983002 & BBa_K2983102 Ura3 promoter
BBa_K2983050 pTef1c: BBa_K2117000 without the BsaI site
BBa_K2983051 pTef1d: wild-type without the BsaI site
BBa_K2983052 pTef1e: wild-type without the BsaI site and shorter
BBa_K2983053 pTef1f: wild-type without the BsaI site, shorter and with CACA before ATG

Terminators

Part Number Part Name
BBa_K2983004 & BBa_K2983104 Ura3 terminator
BBa_K2983055 Lip2 terminator

Golden Gate adapters

Part Number Part Name
BBa_K2983010 Loop Alpha-A
BBa_K2983011 Loop F-Beta
BBa_K2983012 Loop Beta-A
BBa_K2983013 Loop F-Gamma
BBa_K2983014 Loop Gamma-A
BBa_K2983015 Loop F-Epsilon
BBa_K2983016 Loop Epsilon-A
BBa_K2983017 Loop F-Omega
BBa_K2983018 Loop A-alpha
BBa_K2983019 Loop Omega-B
BBa_K2983020 Loop B-Alpha
BBa_K2983021 Loop Omega-C
BBa_K2983022 Loop C-Alpha
BBa_K2983023 Loop Omega-E
BBa_K2983024 Loop E-Alpha
BBa_K2983025 Loop Omega-F

Yarrowia lipolytica genome insertion sequences

Part Number Part Name
BBa_K2983000 Zeta up
BBa_K2983001 Zeta down

and other cloning sites and scars

Part Number Part Name
BBa_K2983006 SfiI
BBa_K2983110 Loop Alpha-A scar after Golden Gate with BsaI
BBa_K2983111 Loop F-Beta scar after Golden Gate with BsaI



During this project, our team designed the following composite parts:


Transcriptional Units

Part Number Part Name
BBa_K2983005 & BBa_K2983105 Ura3 selection marker
BBa_K2983070 hrGFP expression cassette under the control of pTef1b promoter (BBa_K2117005) and Lip2 terminator
BBa_K2983071 hrGFP expression cassette under the control of pTef1a (BBa_K2117000) promoter and Lip2 terminator
BBa_K2983072 hrGFP expression cassette under the control of pTef1c (BBa_K2983050) promoter and Lip2 terminator
BBa_K2983073 hrGFP expression cassette under the control of pTef1e (BBa_K2983052) promoter and Lip2 terminator
BBa_K2983074 RedStar2 expression cassette under the control of pTef1a (BBa_K2117000) promoter and Lip2 terminator
BBa_K2983075 RedStar2 expression cassette under the control of pTef1c (BBa_K2983050) promoter and Lip2 terminator
BBa_K2983076 RedStar2 expression cassette under the control of pTef1d (BBa_K2983051) promoter and Lip2 terminator
BBa_K2983077 RedStar2 expression cassette under the control of pTef1e (BBa_K2983052) promoter and Lip2 terminator
BBa_K2983078 RedStar2 expression cassette under the control of pTef1f (BBa_K2983053) promoter and Lip2 terminator
BBa_K2983081 FadX of Punica granatum expression cassette under the control of pTef1e (BBa_K2983052) promoter and Lip2 terminator
BBa_K2983082 FadX of Trichosanthes kirilowii expression cassette under the control of pTef1e (BBa_K2983052) promoter and Lip2 terminator

Vectors for a Type IIS RFC[1000] Loop assembly system for Yarrowia lipolytica

Part Number Part Name
BBa_K2983030 YL-pOdd1
BBa_K2983031 YL-pOdd2
BBa_K2983032 YL-pOdd3
BBa_K2983033 YL-pOdd4
BBa_K2983034 YL-pOdd5
BBa_K2983035 YL-pOdd6
BBa_K2983036 YL-pEven1
BBa_K2983037 YL-pEven2
BBa_K2983038 YL-pEven3
BBa_K2983039 YL-pEven4

Yarrowia lipolytica genome integration cassettes

Part Number Part Name
BBa_K2983170 hrGFP expression cassette (BBa_K2117005) in YL-pOdd1
BBa_K2983171 hrGFP expression cassette (BBa_K2983071) in YL-pOdd1
BBa_K2983172 hrGFP expression cassette (BBa_K2983072) in YL-pOdd1
BBa_K2983173 hrGFP expression cassette (BBa_K2983073) in YL-pOdd1
BBa_K2983174 RedStar2 expression cassette (BBa_K2983074) in YL-pOdd1
BBa_K2983175 RedStar2 expression cassette (BBa_K2983075) in YL-pOdd1
BBa_K2983176 RedStar2 expression cassette (BBa_K2983076) in YL-pOdd1
BBa_K2983177 RedStar2 expression cassette (BBa_K2983077) in YL-pOdd1
BBa_K2983178 RedStar2 expression cassette (BBa_K2983078) in YL-pOdd1
BBa_K2983181 FadX of Punica granatum expression cassette (BBa_K2983080) in YL-pOdd1
BBa_K2983182 FadX of Trichosanthes kirilowii expression cassette (BBa_K2983081) in YL-pOdd1
Our Sponsors
Contact Us