During this project, our team designed the following basic parts:
Coding sequences
Part Number | Part Name |
---|---|
BBa_K2983003 & BBa_K2983103 | Ura3 |
BBa_K2983060 | RedStar2 |
BBa_K2983061 | FadX of Punica granatum |
BBa_K2983062 | FadX of Trichosanthes kirilowii |
BBa_K2983063 | putative FadX of Jacaranda mimosifolia |
Promoters
Part Number | Part Name |
---|---|
BBa_K2983002 & BBa_K2983102 | Ura3 promoter |
BBa_K2983050 | pTef1c: BBa_K2117000 without the BsaI site |
BBa_K2983051 | pTef1d: wild-type without the BsaI site |
BBa_K2983052 | pTef1e: wild-type without the BsaI site and shorter |
BBa_K2983053 | pTef1f: wild-type without the BsaI site, shorter and with CACA before ATG |
Terminators
Part Number | Part Name |
---|---|
BBa_K2983004 & BBa_K2983104 | Ura3 terminator |
BBa_K2983055 | Lip2 terminator |
Golden Gate adapters
Part Number | Part Name |
---|---|
BBa_K2983010 | Loop Alpha-A |
BBa_K2983011 | Loop F-Beta |
BBa_K2983012 | Loop Beta-A |
BBa_K2983013 | Loop F-Gamma |
BBa_K2983014 | Loop Gamma-A |
BBa_K2983015 | Loop F-Epsilon |
BBa_K2983016 | Loop Epsilon-A |
BBa_K2983017 | Loop F-Omega |
BBa_K2983018 | Loop A-alpha |
BBa_K2983019 | Loop Omega-B |
BBa_K2983020 | Loop B-Alpha |
BBa_K2983021 | Loop Omega-C |
BBa_K2983022 | Loop C-Alpha |
BBa_K2983023 | Loop Omega-E |
BBa_K2983024 | Loop E-Alpha |
BBa_K2983025 | Loop Omega-F |
Yarrowia lipolytica genome insertion sequences
Part Number | Part Name |
---|---|
BBa_K2983000 | Zeta up |
BBa_K2983001 | Zeta down |
and other cloning sites and scars
Part Number | Part Name |
---|---|
BBa_K2983006 | SfiI |
BBa_K2983110 | Loop Alpha-A scar after Golden Gate with BsaI |
BBa_K2983111 | Loop F-Beta scar after Golden Gate with BsaI |