Team:Evry Paris-Saclay/Composite Part

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During this project, our team designed the following composite parts:


Transcriptional Units

Part Number Part Name
BBa_K2983005 & BBa_K2983105 Ura3 selection marker
BBa_K2983070 hrGFP expression cassette under the control of pTef1b promoter (BBa_K2117005) and Lip2 terminator
BBa_K2983071 hrGFP expression cassette under the control of pTef1a (BBa_K2117000) promoter and Lip2 terminator
BBa_K2983072 hrGFP expression cassette under the control of pTef1c (BBa_K2983050) promoter and Lip2 terminator
BBa_K2983073 hrGFP expression cassette under the control of pTef1e (BBa_K2983052) promoter and Lip2 terminator
BBa_K2983074 RedStar2 expression cassette under the control of pTef1a (BBa_K2117000) promoter and Lip2 terminator
BBa_K2983075 RedStar2 expression cassette under the control of pTef1c (BBa_K2983050) promoter and Lip2 terminator
BBa_K2983076 RedStar2 expression cassette under the control of pTef1d (BBa_K2983051) promoter and Lip2 terminator
BBa_K2983077 RedStar2 expression cassette under the control of pTef1e (BBa_K2983052) promoter and Lip2 terminator
BBa_K2983078 RedStar2 expression cassette under the control of pTef1f (BBa_K2983053) promoter and Lip2 terminator
BBa_K2983081 FadX of Punica granatum expression cassette under the control of pTef1e (BBa_K2983052) promoter and Lip2 terminator
BBa_K2983082 FadX of Trichosanthes kirilowii expression cassette under the control of pTef1e (BBa_K2983052) promoter and Lip2 terminator

Vectors for a Type IIS RFC[1000] Loop assembly system for Yarrowia lipolytica

Part Number Part Name
BBa_K2983030 YL-pOdd1
BBa_K2983031 YL-pOdd2
BBa_K2983032 YL-pOdd3
BBa_K2983033 YL-pOdd4
BBa_K2983034 YL-pOdd5
BBa_K2983035 YL-pOdd6
BBa_K2983036 YL-pEven1
BBa_K2983037 YL-pEven2
BBa_K2983038 YL-pEven3
BBa_K2983039 YL-pEven4

Yarrowia lipolytica genome integration cassettes

Part Number Part Name
BBa_K2983170 hrGFP expression cassette (BBa_K2117005) in YL-pOdd1
BBa_K2983171 hrGFP expression cassette (BBa_K2983071) in YL-pOdd1
BBa_K2983172 hrGFP expression cassette (BBa_K2983072) in YL-pOdd1
BBa_K2983173 hrGFP expression cassette (BBa_K2983073) in YL-pOdd1
BBa_K2983174 RedStar2 expression cassette (BBa_K2983074) in YL-pOdd1
BBa_K2983175 RedStar2 expression cassette (BBa_K2983075) in YL-pOdd1
BBa_K2983176 RedStar2 expression cassette (BBa_K2983076) in YL-pOdd1
BBa_K2983177 RedStar2 expression cassette (BBa_K2983077) in YL-pOdd1
BBa_K2983178 RedStar2 expression cassette (BBa_K2983078) in YL-pOdd1
BBa_K2983181 FadX of Punica granatum expression cassette (BBa_K2983080) in YL-pOdd1
BBa_K2983182 FadX of Trichosanthes kirilowii expression cassette (BBa_K2983081) in YL-pOdd1
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