Team:Costa Rica/Parts

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Here we enlist the parts we submitted to the iGEM repository. These sequences refer to the parts we used to test possible systems to detect and eradicate Clostridiodes difficile.

All our parts were de-novo synthesized, the following composite parts describe the function of each device and basic parts that partially constitute them.

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Lysin
AIP
Terminators

BBa_K2978000

Inducible protein that causes lysis of the pathogen Clostridioides difficile

Name Type Description Designer Length
BBa_K2978001 Operator DNA binding site for LacI protein Costa Rica 25 bp
BBa_K2978002 Spacer DNa Spacer Costa Rica 6 bp
BBa_K2978003 Coding Causes cell lysis of the pathogen Clostridioides difficile Costa Rica 561 bp
BBa_K2978004 Spacer DNA Spacer Costa Rica 8 bp

BBa_K2978100

Quorum senging artificial induction of Clostridioides difficile

Name Type Description Designer Length
BBa_K2978101 Coding AgrB Clostridioides difficile gene Costa Rica 585 bp
BBa_K2978102 RBS Strong RBS Costa Rica 18 bp
BBa_K2978103 Promoter Reverse Complement of pLac inducible promoter Costa Rica 54 pb
BBa_K2978104 Spacer DNA Spacer Costa Rica 21 bp
BBa_K2978105 Coding AgrB Clostridioides difficile gene Costa Rica 144 bp
BBa_K2978106 Spacer DNA Spacer Costa Rica 10 bp

Terminators Measurement

Terminators proposed to be use in Lactobacillus spp

BBa_K2978201

BBa_K2978202

BBa_K2978203

BBa_K2978204

BBa_K2978205

Name Type Description Designer Length
BBa_K2978206 Promoter Lactobacillus spp Slp constitutive promoter Costa Rica 281 bp
BBa_K2978207 RBS Lactobacillus spp Ribosomal Binding Site Costa Rica 17 bp
BBa_K2978208 Reporter Codon optimized GFP for Lactobacillus spp Costa Rica 720 bp
BBa_K2978209 Spacer DNA Spacer Costa Rica 20 bp
BBa_K2978210 Reporter Codon optimized RFP for Lactobacillus spp Costa Rica 681 bp
BBa_K2978211 Terminator 667 Terminator for Lactobacillus spp Costa Rica 67 bp
BBa_K2978212 Terminator 908 Terminator for Lactobacillus spp Costa Rica 73 bp
BBa_K2978213 Terminator NisA Terminator for Lactobacillus spp Costa Rica 74 bp

"La ciencia es lo que entendemos lo suficientemente bien como para explicarle a una computadora. Arte es todo lo demás que hacemos".

— Donald Knuth.