Team:Costa Rica/Attributions

In order to achieve our project’s goal, we distributed different tasks and activities among the team members. We also received lots of support from people outside the team, which was extremely appreciated. In the following lines we present the work done by each of the people involved in our project.

Attributions

Acknowledgements

Idea and Brainstorming

• Anthony, María Jose, Noé, Diego, Paula and Pablo: were in charged of brainstorming about different problems, doing research about the topic, proposing a solution by the use synthetic biology and designing a draft of the genetic constructs to be needed.

• David and Stefany (PIs): mentored us on the process and gave us feedback.

Project Development

• Pablo and Noé: designed the genetic sequences that were ordered.

• Anthony, María Jose, Noé, Diego, Paula and Pablo: checked all the genetic sequences, searched the protocols used and designed the experiments tested.

• Diego and Adrián: modeled the different linkers for the chemeric AgrA.

• Ignacio: designed the mathematical model of the whole system.

• Pablo, Paula and María José: worked in the wet lab. They were responsible of genetic assemblies and protein extraction and purification.

• Anthony, Noé and Diego: worked in the wet lab. They were in charged of transforming Lactobacillus casei and performing the assays in Clostridium difficile.

• Adriana, Argerie, Andrés and Ana María (pre-iGEMers): helped us in some wet lab tasks, such as preparing culture media and reagents.

• Juliana: made our project look amazing. She designed our wiki, presentations, poster, videos and images.

• Adrián: programed the codes for our wiki.

• David and Stefany (PIs): carried out all the administrative processes we needed in our universities. They also guided us in the development of the whole project, lent us their laboratories and some reagents and helped us get in contact with other experts.

• Carlos Quesada y César Rodríguez (advisors): provided us with the two strains of C. difficile with which we worked.

• Elías Barquero (instructor): lent us his lab and equipment to work with Clostridium difficile. He also gave us advises on how to work with that microorganism.

• Silvia Arce (instructor): advised us on the protocols for protein extraction and purification.

• Erick Hernández (advisor): lent us his laboratory and equipment for protein assays. He also mentored us on how to obtain our protein of interest.

• Kevin (advisor): explained to the team how to modify the wiki and advised us on how to distribute the wiki related tasks in order to have a less stressed wiki freeze.

• Abad Rodríguez (advisor) and Carolina Sancho (advisor: helped us get permission to work in the laboratories of the National University and get some reagents.

• Silver (advisor): gave us feedback on which AgrAs we had to test.

• Samantha (advisor): checked our proposal of characterization and gave us feedback.

• Jean Carlo Salas and Kevin: answered all the wiki related questions regarding code.

Human Practices

• María José, Paula and Pablo: prepared all the materials and documents needed for the Integrated Human Practice.

• Anthony, María José, Noé, Paula and Pablo: sampled 100 people for the Integrated Human Practice.

• Diego: analysed the data generated in the Integrated Human Practice.

• Carolina P. (advisor): helped us get in contact with different decision takers and experts on biopolicy. She also advised us on how to design our Public Engagement Human Practice.

• María José, Paula and Pablo: collected information about biopolicy through interviews with different experts.

• María José: contacted the Geriatrics and Gerontology Hospital.

• All team members: searched information about biopolicy in Costa Rica Md other nations in order to generate the material used for the Public Engagement Human Practice. Also, we presented that information to several deputies.

Collaborations

• María José: got in touch with the Tübingen and TAS Taipei iGEM team, in order to design and organize the Sustainable Development Goals (SDG) Challenge.

• Pablo Vargas (instructor): proposed the idea of mapping how iGEM projects are related with SDGs and helped us get in contact with Tübingen team to start our collaboration.

• Juliana: worked on the design line for the SDG Challenge.

• Kevin (advisors): created the tool that generated the collage in the SDG Challenge.

• Noé took a water sample from a river to help the UPNA iGEM team.

• María José: was in charge of international relationships.

Funding

• Stefany and David (PIs): helped us get in contact with different enterprises to get monetary funding as well as reagents and lab supplements.

• María José, Pablo and Paula: got in touch with people and enterprises willing to donate.

• Anthony and María José: organized different events for people to contribute to the project.

Research assistants

We want to give special thanks to our passionate pre iGEMers who helped us in the wet lab:

Andrés Carranza
Ana María Ocampo
Argerie Oviedo
Adriana Fallas
Joel Rojas

Photographers

Also, we want to thank the people who captured our team essence:

Daniel Better Leal
Melania Martínez

Donations

DiffEasy couldn’t been possible without the help of family, friends and all the people who saw our project and give us a hand. Thank you, every donation help us in a big way: