Team:Bilkent-UNAMBG/Contribution
Characterization on Bba_K1741014
We characterized the ProD-sfGFP part (Bba_K1741014) by cloning this part into pZA, pZE and pZS backbone. We obtained the fluorescence data by measuring the fluorescence level for each 30 minutes for 6 hours. Also, we showed the growth curve of the cell containing the different origin plasmids with the different strains. We did not observe any significant differences between different strains within the context of fluorescence and OD. RFU/OD graphics illustrates the relative fluorescence units per optical density which depicts the fluoresce which are expressed in a certain unit relatively to cell growth.In Figure 1, 3, 5 and 8, RFU/OD600 are shown. The graphs are almost linear as expected, since fluorescence intensity are expected to increase with increasing cell number.
Comparing growth curves given in Figure 2,4,6 and 7, it is seen that cells including the plasmids reached a higher OD600 value.
