Team:Bilkent-UNAMBG/Basic Part
Basic Parts
Our candidate for the best new basic part is our single chain insulin analog with a linker peptide. This single chain insulin has modifications to its A and B chains to increase its bioactivity. It also has a linker between the A and B chains which promotes the folding of the peptide. The modified A and B chains of insulin are called SCI-57 and the linker is one of the many discussed in the paper Rajpal et al.
We have shown that the protein (Rajpal 15 design with SCI57 a-b chains) has been expressed via western blot. We supported the western blot data with an ICC assay. Where we visualised our his tag containing proteins.
Rajpal 15 design with SCI57 a-b chains
References
1) Rajpal, G., Liu, M., Zhang, Y., & Arvan, P. (2009). Single-chain insulins as receptor agonists. Molecular endocrinology (Baltimore, Md.), 23(5), 679–688. doi:10.1210/me.2008-0349
2) Hua, Q. X., Nakagawa, S. H., Jia, W., Huang, K., Phillips, N. B., Hu, S. Q., & Weiss, M. A. (2008). Design of an active ultrastable single-chain insulin analog: synthesis, structure, and therapeutic implications. The Journal of biological chemistry, 283(21), 14703–14716. doi:10.1074/jbc.M800313200