Team:BUCT-China/Notebook

Electron Microscope
15 May.-19 May.

Surprisingly, we found some cracks on the surface of PE affected by the marine bacteria.

Constract Gene Circle Map
19 May. -27 May.

Sequenced the entire genome of the marine bacteria.
Adopted gene annotation and predict gene function.
We successfully built the phylogenetic trees.

GO Analysis
27 May.-10 Jun.

Through GO analysis, it could be speculated that the bacteria have a vigorous metabolic process and a large amount of enzyme production.

Transduction Experiment
10 Jun.–23 Jun.

Plasmid synthesis and plasmid transformation.
The host cells:E.coli

Verify The Degradation Ability
23 Jun. –15 Jul.

Mainly adopted the NMR and the chromatographic analysis
We have obtained about 18-20 carbon chains after the degradation.

Biological Characterization
15 Jul. – 1 Aug.

We got three new parts and finished the biological characterization.

Collaboration
1 Sept. – 7 Oct.

Communicated with the iGEMers in Lanzhou University. We finally reached an agreement in the cooperative proposal.

Human Practices
8 Oct. – 19 Oct.

We visited waste treatment plant in Beijing to know the present situation of plastic waste disposal.
Also we held some recycling activities in our school and made a presentation about our project, appealing for environmental protection.

Team:BUCT-China/Notebook

Electron Microscope15 May.-19 May.

Constract Gene Circle Map 19 May. -27 May.

GO Analysis27 May.-10 Jun.

Transduction Experiment 10 Jun.–23 Jun.

Verify The Degradation Ability 23 Jun. –15 Jul.

Biological Characterization15 Jul. – 1 Aug.

Collaboration 1 Sept. – 7 Oct.

Human Practices 8 Oct. – 19 Oct.