Team:BUCT-China/Collaborations
Collaborations
Our team scanned some of the iGEM team's project titles and abstracts, and we were interested in the project of Lanzhou University, which proposed a treatment for pancreatic cancer through protein-protein interactions. We got in touch with the iGEM team of Lanzhou University through the QQ group of iGEM in China. Our two teams held a online meeting and shared the introduction of our respective projects, and then through a series of discussions, we put forward some suggestions to each other, which is helpful to the implementation and future prospect of our two projects.
Our suggestions
After learning about project of iGEM team of Lanzhou University , we made some suggestions on the selection of enzymes.We suggest that compared with intestinal peptidases, it is better to use TEVP enzymes to cleave target sequences between ALKBH5 to synthesize the active protein. Although intestinal peptidases are highly specific, their efficiency is low, and the cost is higher.Out of consideration for the effectiveness and the cost, we advise their team to use TEVP instead of intestinal peptidases.
The suggestion they give us
The iGEM team of Lanzhou University listened to our research on oxidase and suggested that we can further increase the production of oxidase by optimizing the promoter in our future research, which can accelerate the degradation of PE as well. They suggested that we can select the promoters that can express more proteins based on the gene function and protein expression data of our strain, and screen these promoters for the first time, then obtain the highly expressed promoters. Through saturation mutation in the-35 to-10 region of the promoter region, we can construct a mutation library, and then through several generations of high-throughput screening we can get the final excellent mutation promoter; We can also get excellent promoters by binding them together, construct a tandem promoter or enhancer sequence structures to construct compound promoters to enhance the expression intensity of promoters in target cells.