Team:BOKU-Vienna/Part Collection
Part Collection
The team BOKU-Vienna created a library of BioBricks that are crucial for the development of a new, fast
and easy-to use diagnostic tool for Buruli ulcer. The biobricks are compatible with most assembly
standards, including RFC10 and RFC1000.
We successfully screened for a riboswitch that will increase transcription of a downstream located gene
in the presence of mycolactone, which is the disease-causing toxin of Buruli ulcer. Further, our
part-collection includes all biobricks that were used to proof the functionality of our system with a
transcriptional theophylline riboswitch.
The designed and constructed system can easily be adapted for detection of various disease-causing
agents by just changing the riboswitch-sequence according to the target to generate a visible
readout.
This highlights that our genetic device could act as a great starting point for the development of a
diagnostic platform for the detection of rare, overlooked and neglected disease-causing agents or even
to screen for toxins in the environment.
| BBa_K3015000 | Mycolactone-Riboswitch |
| BBa_K3015001 | pMyco-RBS |
| BBa_K3015002 | Myco-RBS-GFP-Term |
| BBa_K3015003 | T7prom-amilCP- |
| BBa_K3015004 | Theophylline_Riboswitch (transcriptional) |
| BBa_K3015005 | pTheo-RBS |
| BBa_K3015006 | T7 RNA Polymerase (No BbsI/BpiI) |
| BBa_K3015007 | pTheo-RBS-GFP-Term |
| BBa_K3015008 | pTheo-RBS-T7-Term |
| BBa_K3015011 | Riboswitch Sensor cassette |
| BBa_K3015012 | T7-Promotor LacOperator RBS |
| BBa_K3015013 | GFP mut3 |
