Team:BGU Israel/Part Collection
In our project we used the Bacillus thuringiensis subsp. israelensis (Bti) toxin subunits.
Some genes of these toxin subunits were used by previous iGEM teams and projects but had various point mutations throughout the DNA sequence. Hence, we added these basic parts again.
After adding our versions of toxin sequences, we designed and added commonly used tags (HA, Strep and His) to our subunits in order to be able to detect them using anti-tags antibodies in Western blot analysis.
The parts containing the tags appear in our designed plasmids (devices), which we used during our project.
Our collection offers modified Bti toxin subunits with common tags, alleviating the need to use specific antibodies to the original subunits, and the ability to use anti-tags antibodies which are more commonly used.
For each of the parts we have demonstrated a successful expression of the different toxin subunits in Serratia marcescens 274, using Western blot analysis.
A link to our final polycistronic plasmid Click here.
Links:
Cry11Aa + Strep tag
Cry4Ba + HA tag
Cyt1Aa + Strep tag
P20 + His tag