Team:BGU Israel/Experiments

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Experiments - Protocols

Bacteria

All the work done with Serratia marscecens bacteria

OD Calibration

Electroporation

Examine survival of Serratia marcescens in mosquito's gut

Transformation

Plasmids

All the work regarding the constructions of our plasmids

Gibson Assembly

Mini Prep

PCR Clean Up / Agarose Gel Extraction

Plasmid Assembly

Mosquitoes

Examining the transfer of bacteria from females and males mosquitoes to the eggs

Hatching mosquito egss and treatment

Vertical Transfer

Horizontal Transfer

Toxicity Assays

Examining the toxicity of the various toxin subunits of our constructed plasmids

Toxicity Assays

Western Blot

Examining the expression of the toxin subunits of our constructed plasmids

Western blot procedures

Bonuses

Various protocols of the "Results- Bonuses" section of our project

Protocols of the "Bonuses" from our project

FlyGEM Team

Ben Gurion University of the Negev

Ben Gurion BLD1, Beer Sheva, Israel

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