Team:ASIJ Tokyo/Part Collection
Parts Collection
A Compilation of Our Submissions
This year our part range was from K2928000 to K2928999. We made a number of new Basic Parts, namely introducing the sequence for the alpha-amylase-inhibiting 0.19 wheat albumin to the Registry, as well as two modifications (E1 and N1) that increased its inhibitory abilities. Additionally, we made constructs to express these 0.19s, with and without secretion tags. We improved on a previous part by adding a promoter of suitable strength to an alpha-amylase with an RBS site (BBa_K523001). As a collection, these parts allowed us to show in our experiments the inhibitory activity of 0.19 and its modified counterparts on alpha-amylase in a realistic system.
| Name | Type | Description | Designer | Length |
|---|---|---|---|---|
| BBa_K2928001 | Basic | Sequence for wheat albumin 0.19, an α-amylase inhibitor | Lauren Hartz | 333bp |
| BBa_K2928002 | Basic | Modified 0.19 Wheat Albumin sequence (Gly to Glu), an α-Amylase inhibitor | Genta Firth | 372bp |
| BBa_K2928003 | Basic | Modified 0.19 Wheat Albumin sequence (Ile to Asn), an α-Amylase inhibitor | Genta Firth | 372bp |
| BBa_K2928004 | Composite | 0.19 Wheat Albumin (α-amylase inhibitor) expression using TDH3, RBS, and ADH3 | Genta Firth | 1222bp |
| BBa_K2928006 | Composite | Modified 0.19 Wheat Albumin (Ile to Asn) with expression using TDH3, RBS, and ADH3 | Lauren Hartz | 1263bp |
| BBa_K2928007 | Composite | 0.19 Wheat Albumin with expression using TDH3, RBS, Secretion Tag, and ADH3 | Lauren Hartz | 1222bp |
| BBa_K2928009 | Composite | Modified 0.19 Wheat Albumin (Gly to Glu) expression using TDH3, RBS, and ADH3 | Lauren Hartz | 1263bp |
| BBa_K2928013 | Composite | Modified 0.19 Wheat Albumin (Gly to Glu) expression using TDH3, Secretion tag, RBS, and ADH3 | Lauren Hartz | 1383bp |
| BBa_K2928014 | Composite | Modified 0.19 Wheat Albumin (Ile to Asn) expression using TDH3, RBS, secretion tag, and ADH3 | Lauren Hartz | 1383bp |
| BBa_K2928015 | Composite | Alpha Amylase (malS) with RBS and Anderson promoter J23101 | Lauren Hartz | 2110bp |