Team:SJTU-BioX-Shanghai/Medals
Our Awards:
Gold Medal
and
Best New Application Project Nominee
Thank you for supporting our team!
Below is our Judging form and Medal criteria summary:
Click HERE to see our judging form.
Bronze
Registration
Our team application was accepted by iGEM Headquarters on 2019-03-26 21:15:09.
Click HERE to see our team's information.
Competition Deliverables
Our wiki page was completed on 21 October. And we are looking forward to show our poster and the presentation in the Giant Jamboree.
Attributions
We have sorted out our work and division of labor, and thanks for everyone who made efforts in the competition.
Click HERE to see our Attributions.
Project Inspiration and Description
We have discussed our inspiration and properly described our project.
Click HERE to see our Description.
Characterization / Contribution
We have added quantitative experimental characterization data to Part BBa_K2558001 and BBa_K2761009.
Sliver
Validated part
We have designed and characterized new Biobrick parts. Examples of this can be see at:
Collaborations
We have worked with six teams, mentor one team and participate in CCiC community in conference and interlab.
Click HERE to see what we’ve done.
Human Practices Silver
Human practices run through our projects as the soul. Based on our project, we have proposed a new human practices model: L-M-P-H-E. We conducted academic dialogue with scholars from different fields. We visited related companies on biological storage and did a market research on storage, which promoted us to finally decide to develop CRISPR storage technology. We conducted a large scale survey and comprehensive statistical analysis on public's attitude towards CRISPR gene editing, learning more about public's worry and expectation on CRISPR technology. Moreover, we also delved into some of the ethical and philosophical issues behind gene editing, warning us to pay more attention to the security and application scope of CRISPR technology in our project. We provided various educational activities as well. We hope to form a good platform for researchers, humanities, companies and the public to communicate with each other, helping to construct a society that can work with biological technology productively and safely.
Want to see more? go to Human Practices SJTU-BioX-Shanghai for more information.
Gold
Integrated Human Practice
Human practices run through our projects as the soul. As gene editing became a focus topics in life science, we conducted a large scale survey and comprehesive statistical analysis on public's attitude towards CRISPR gene editing. Realising the fact that the public is generally worried about the safety of gene editing, we decided to develop a detection and reporting system for gene editing off-target incidents to improve the safety of gene editing technology. Besides, the divided opinions we knew from the survey indicate the huge controversy in society about gene editing. So we conducted depth research on philosophy and law about gene editing and finally choose to edit simple prokaryotes that could promote to the application and acceptance of CRISPR system but with few controversies and risks. Therefore, based on recording off-target signals, we further propose a general information recording and reading system based on CRISPR. And after academic dialogue with scholars from different fields and visit to Hongxun Biotech Company, we gave up using sequencing as a means of reading information, and determinated to use fluorescence as a report to achieve a real-time recording and reading system.
Want to see more? go to Human Practices SJTU-BioX-Shanghai for more information.
Project modeling
The design of lure sequence is the theoretical basis of the off-target detection. In order to evaluate the binding ability of dCas9 to lure sites, we established a kinetic model to calculate the probability of off-target. We also compiled a relevant software for calculation to guide the experimental design of the best lure sequence. What's more, we believe that the storage should not be limited to the off-target information, but can be extended to a broader binary world. Considering that bio-storage may have low probability of error, we have designed a set of coding specifications. In the software, we achieved maximum fault tolerance with minimal redundancy by mapping the dataset to the Galois field. We also wrote a codec software that allow all the people to explore the infinite possibilities of bio-storage.
Click HERE to see our model.
Improve previous part
This year, we had greatly expanded the application scope of BBa_K2761009 and created a new part BBa_K2996800. HindIII is a common enzyme cleavage site. By using point mutation, the HindIII restriction site is changed, so we can use other HindIII restriction sites on the plasmid backbone without any effect of the functional protein Cas1-Cas2. For more about the part improvement, click HERE.
Demonstrate
We have combined relevant wet lab experiments with modeling and human practice. And the experimental results show that our project works in compliance with safety.
Go to the Demonstrate page and check it.
SJTU-BioX-Shanghai
Contact us: sjtuigem@gmail.com
Bio-X Institute, Shanghai Jiao Tong University, Dongchuan Rd. 800