Team:ShanghaiFLS China

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Pichia Pastoris GS115:

The Methanol Converter

Methanol is a major byproduct of the coal industry. Engineered Pichia pastoris GS115 is capable of converting methanol into medical compounds such as the insulin precursor and lovastatin. However, this metabolism pathway is highly specific and results in significant oxygen consumption and heat generation, which has limited its industrial applications. We addressed this issue by improving the methanol conversion rate in P. pastoris by rewiring its homogeneous circuits expressing the transcription factors that would up-regulate the expression of AOX1, the protein that allows it to metabolize methanol. Through multiple rounds of experiments, we eventually acquired strains that are able to yield an up to 20% increase in total production per gram methanol compared to the wildtype.

Design Experiment Results Parts

About us

We are a diverse group of high school students from China, studying in Beijing, Tianjin, the United States or Switzerland, as well as in Shanghai Foreign Language School. We have different hobbies, ambitions, and views towards bioengineering. We also have different jobs o realize this complicated project. For more information about us, please click on the links below:

Team Members Attributions