In our project, we rewired the homogeneous regulatory pathways of Pichia pastoris GS115 to improve its methanol metabolizing capabilities. When we consulted research professionals and industry leaders regarding the feasibility of our approach in industrial settings, most were not optimistic. To address this doubt, we constructed a model calculating the oxygen consumption and heat generation of our yeast strains, two main factors to be considered in industrial processes. We based our models on validated and published equations and data from our experiments. The results of our calculations confirm the potential industrial applications of our modified P. pastoris GS115 strains.
