Team:Calgary/Demonstrate
Demonstration of our work
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Our team’s goal was to create a new method for chlorophyll removal from green seed canola oil. We also sought to repurpose the chlorophyll removed from the oil into pheophorbide a, an experimental anti-fungal treatment. After months of hard work and experimentation, our team was able to demonstrate the following:
- Our water soluble chlorophyll-binding protein, 6GIX, can be produced and purified
- 6GIX can be emulsified and can be used to remove chlorophyll from canola oil
6GIX Production
To show we produced 6GIX in significant amounts, we ran SDS-PAGE gel which showed the right bands. We purified the protein using 6X His affinity chromatography and showed bands of the correct size which also confirms successful production. We also did a Western Blot. We also measured the absorption of the protein at 200nm to quantify that we have THICC amounts.
Emulsification and Extraction
We proved that our emulsification system successfully reduces the amount of chlorophyll in canola oil. with this THICC experiment