Team:Fudan-TSI/Parts

Parts Overview

This year Team Fudan-TSI presents you with a toolbox for our in vivo sequence-specific continuous mutagenesis system(R-Evolution). This toolbox includes promoters, RBSs, terminators, CDSs and other regulatory parts which undergo careful design and optimization. The Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT), which is new to the registry, and Cre Recombinase are the most important components of our system for which we have put emphasis on the construction and characterization of these two parts. Also, our improved lac repressor protein is fully equipped for regulating protein expression.

References

[1] Wen-Hui Zhang, Evguenia S. Svarovskaia, Rebekah Barr, and Vinay K. Pathak. Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine–thymine tracts[J]. Proc Natl Acad Sci U S A. 2002 Jul 23; 99(15): 10090–10095