Team:IISER Kolkata/Experiments

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Experiments

Bacterial protocol

1

LB media preparation

2

SOB media preparation

3

50X TAE buffer

4

CCMB buffer preparation

5

Calcium chloride competent cell preparation

6

Hanahan competent cell preparation

7

Electrocompetent cell preparation

8

Heat shock transformation of chemically competent cells

9

Transformation by electroporation