Difference between revisions of "Team:Marburg"

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          <img src="https://static.igem.org/mediawiki/2019/b/b9/T--Marburg--m_logo-text.jpg" alt="Syntex Logo" style="width: auto; height: 7.5rem;">
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        </figure>
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<h1> Welcome to iGEM 2019! </h1>
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      </div>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<img src="https://placehold.it/1080x320">
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        <h1 class="title">
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          Our Project
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            <p style="margin-bottom: 1rem;">
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              With rising atmospheric CO<sub>2</sub> concentrations and declining oil reserves,
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              we saw that the worldwide effort to change from a petroleum based industry to a carbon neutral industry needs to increase drastically.
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<h3>Before you start</h3>
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              One
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<p> Please read the following pages:</p>
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              of the most promising key technologies right now is the use of phototrophic organisms for biotechnological applications. Hence, we
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<ul>
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              decided
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<li> <a href="https://2019.igem.org/Competition">Competition Hub</a> </li>
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              quite early this year to devote ourselves to a photosynthetic project. During the design phase, which we initially thought about a
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<li> <a href="https://2019.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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              project
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<li> <a href="https://2019.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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              around the model moss <i>Physcomitrella patens</i>, we soon stumbled upon many common obstacles characteristic to phototrophic chassis
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</ul>
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              due
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</div>
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              to our choice of organism. Issues like time intensive culturing and complicated techniques to perform basic molecular biological methods
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              eventually showed us, why only very few iGEM teams every year decide to use a phototrophic chassis. We saw a need to tackle these issues
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              and were determined to find a solution.
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<div class="clear extra_space"></div>
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              Consequently, our choice fell on the cyanobacterial strain <i>Synechococcus elongatus</i> UTEX 2973, due to its highly potential
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              doubling
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              time of about 2 hours <sup>1</sup>. This could have a huge impact, as the time consumed by many workflows is mainly dictated by the
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              growth
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              of your chassis. Our strain could compete with common heterotrophic chassis like yeast, which would be a novelty in photosynthetic
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              research. We are dedicated to develop this strain as a chassis for the scientific community and future iGEM teams. To restore its
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<div class="column full_size" >
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              natural
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<h3> Styling your wiki </h3>
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              competence, which it has lost after isolation, we will integrate a CRISPR/Cpf1 system into our toolbox, enabling easy genomic
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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              manipulation
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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              and thus giving us the tools to construct various strains and revert the point mutation responsible for the loss of natural competence.
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              Additionally, we remove the wild type plasmid pANS from <i>Synechococcus</i> to use it’s origin of replication in our “Marburg
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</div>
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              Collection
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              2.0”: a versatile Golden Gate based modular cloning library for fast state of the art assembly of genetic constructs based on a “one
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              step
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              - one pot” reaction. By designing “operon connectors”, our toolbox is the first to assemble complete operons in the span of two days.
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              This
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<div class="clear extra_space"></div>
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              assembly technique can be performed in our open source liquid handler OT-2 from Opentrons, paving the way for our vision of fully
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              automated cloning in molecular and synthetic biology - from ordered primers to the finished construct. To add to this vision, we are the
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              first to establish several laboratory practices in this robot such as colony picking, plating and plasmid purification. By making full
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              cloning processes possible in the Opentron environment, we give iGEM teams access to an affordable way to accelerate their undertaking,
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<div class="column third_size" >
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              allowing them to allocate more time to the design of their project. In our metabolic engineering project we prove the value of the tools
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              we hereby provide: Using our cloning system, we modify our established chassis to produce limonene and farnesene, two valuable
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<h3> Uploading pictures and files </h3>
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              biochemicals that can be used as a biofuel. The chassis’ capabilities are not limited to terpene production but can be expanded to other
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<p> You must upload any pictures and files to the iGEM 2019 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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              areas of biotechnological applications as well as to academic experimental setups. Customized strains offer the opportunity of
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              sustainable
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              growth in drug development and manufacturing, helping us all to achieve our vision of a more sustainable future on this planet.
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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            </p>
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            <p>
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<div class="button_link">
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              <sup>1</sup> Yu, J.; Liberton M.; Cliften, P. F.; Head, R. D.; Jacobs, J. M.; Smith, R. D.; Koppenaal, D. W.; Brand J. J.; Pakrasi, H.
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<a href="https://2019.igem.org/Special:Upload">
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              B.:
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UPLOAD FILES
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              <i>Synechococcus elongatus</i> UTEX 2973, a
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</a>
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              fast growing cyanobacterial chassis for
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</div>
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              biosynthesis using light and CO<sub>2</sub>. Scientific Reports. 5:8132. DOI: 10.1038/srep08132 (2015)
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            <figure class="image">
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<h3> Wiki template information </h3>
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              <img src="https://static.igem.org/mediawiki/2019/d/d4/T--Marburg--m_team.jpg" alt="Team">
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2019.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<div class="column third_size" >
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<div class="highlight decoration_B_full">
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<h3> Editing your wiki </h3>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<div class="button_link">
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<a href="https://2019.igem.org/wiki/index.php?title=Team:Marburg&action=edit">
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EDIT PAGE
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</a>
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
 +
<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
 +
<li>Be clear about what you are doing and how you plan to do this.</li>
 +
<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
 +
<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
 +
<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
 +
<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2019.igem.org/Calendar">iGEM 2019 calendar</a> </li>
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<li>Have lots of fun! </li>
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</ul>  
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</div>
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<div class="column third_size">
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<div class="highlight decoration_A_full">
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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</ul>
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{{Marburg/footer}}
 
{{Marburg/footer}}

Revision as of 11:32, 27 July 2019

Welcome to iGEM 2019!

Your team has been approved and you are ready to start the iGEM season!

Before you start

Please read the following pages:

Styling your wiki

You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.

While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.

Uploading pictures and files

You must upload any pictures and files to the iGEM 2019 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.

When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)

UPLOAD FILES

Wiki template information

We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!

Editing your wiki

On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!

Use WikiTools - Edit in the black menu bar to edit this page

EDIT PAGE

Tips

This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:

  • State your accomplishments! Tell people what you have achieved from the start.
  • Be clear about what you are doing and how you plan to do this.
  • You have a global audience! Consider the different backgrounds that your users come from.
  • Make sure information is easy to find; nothing should be more than 3 clicks away.
  • Avoid using very small fonts and low contrast colors; information should be easy to read.
  • Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2019 calendar
  • Have lots of fun!

Inspiration

You can also view other team wikis for inspiration! Here are some examples: