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− | After we determined the light intensity via this method the doubling time of our strain reduced drastically from two hours to 90 minutes. | + | After we determined the light intensity via this method the doubling time of our strain reduced drastically from two hours to under 80 minutes. |
− | We believe that the standardization of measuring light intensity has a huge impact in the field of phototrophic biology. What we often time stumbled upon when we were looking for literature on our iGEM project was that the information on light intensity in these papers were often inconsistent. Oftentimes the only values on the intensity were given in the unit µEinstein, but the needed details on how that number was measured, was missing. So some people would measure the intensities with a planar device, others would determine them via a spherical quantum sensor. | + | We believe that the standardization of measuring light intensity has a huge impact in the field of phototrophic biology. What we often time stumbled upon when we were looking into literature was that the information on light intensity in these papers were often inconsistent. Oftentimes the only values on the intensity were given in the unit µEinstein, but the needed details on how that number was measured, was missing. So some people would measure the intensities with a planar device, others would determine them via a spherical quantum sensor. |
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− | During our skype call with James Golden he emphasized that a lot of experiments are simply not reproducible, because there is no way to tell how much light one has to expose their organisms to. Additionally, we got the feedback of Dr. Nicolas Schmelling that even professional cultivation devices from companies which are specialized on building them, cannot deliver consistent and even illumination.
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| To go even further, we think that the spectrum of the respective lamp should also be considered when talking about standardization. The light spectrum of our two lamps look as illustrated by Fig. 1. Even though the standardization of the light quality seems to be a very hard task it should still be included in scientific works in order to give as much information as possible about the experimental setup. | | To go even further, we think that the spectrum of the respective lamp should also be considered when talking about standardization. The light spectrum of our two lamps look as illustrated by Fig. 1. Even though the standardization of the light quality seems to be a very hard task it should still be included in scientific works in order to give as much information as possible about the experimental setup. |
| <center><figure Style="text-align:center"> | | <center><figure Style="text-align:center"> |
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| <img src="https://static.igem.org/mediawiki/2019/1/14/T--Marburg--wackelpudding2.png" style="mix-blend-mode: multiply;"> | | <img src="https://static.igem.org/mediawiki/2019/1/14/T--Marburg--wackelpudding2.png" style="mix-blend-mode: multiply;"> |
| <figcaption> | | <figcaption> |
− | Fig. 1: Light distribution inside our incubator | + | Fig. 1: Light distribution inside our incubator setup. |
| </figcaption> | | </figcaption> |
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− | We measured an equidistant grid of points at which we measured the average amount of photons (10 seconds) to minimize fluctuation. These data points were then interpolated with the help of a b spline surface to predict the amount of µmol photons at any given point of the incubator. This method is described in more detail on our <a href="https://2019.igem.org/Team:Marburg/Model">model</a> page. We believe that the standardization of measuring light intensity has a huge impact in the field of phototrophic biology and immensely helps to create reproducible experimental setups. | + | We measured an equidistant grid of points at which we measured the average amount of photons (10 seconds) to minimize fluctuation. These data points were then interpolated with the help of a b spline surface to predict the amount of µmol photons at any given point of the incubator. This method is described in more detail on our <a href="https://2019.igem.org/Team:Marburg/Model" target="_blank">model</a> page. We believe that the standardization of measuring light intensity has a huge impact in the field of phototrophic biology and immensely helps to create reproducible experimental setups. |
| </p> | | </p> |
| <p style="text-align: justify; margin-bottom: 1em;"> | | <p style="text-align: justify; margin-bottom: 1em;"> |