Difference between revisions of "Team:Rice/Model"
| Line 166: | Line 166: | ||
<div class="row" style = "background-color:#26849c!important;"> | <div class="row" style = "background-color:#26849c!important;"> | ||
</div><h1 style = "background-color:#1f9ec1!important;"> How RNA Thermometers Regulate Translation</h2> </br> | </div><h1 style = "background-color:#1f9ec1!important;"> How RNA Thermometers Regulate Translation</h2> </br> | ||
| − | + | <p>In essence, RNA thermometers are a form of temperature dependent translational regulation. At low temperatures, there is a higher probability of more base pairs forming what is known as a "stem-loop structure". At higher temperatures, the thermometers "melt", meaning there is a decreased likelihood of base pairs forming. </p> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | <p>In essence, RNA thermometers are a form of temperature dependent translational regulation. At low temperatures, there is a higher probability of more base pairs forming what is known as a "stem-loop structure". At higher temperatures, the thermometers "melt", meaning there is a decreased likelihood of base pairs forming. </p> | + | |
</div> | </div> | ||
| + | <img class="img-responsive" style="width:100%" src="https://static.igem.org/mediawiki/2019/8/8c/T--Rice--thermometerlocky.svg"/> | ||
| + | |||
| + | </div> </div> | ||
| + | <div class="row" style = "background-color:#f6c374!important;"> | ||
| + | |||
</div><h1 style = "background-color:#f6c374!important;">RNA Thermometer Design</h2> </br> | </div><h1 style = "background-color:#f6c374!important;">RNA Thermometer Design</h2> </br> | ||
| Line 178: | Line 179: | ||
</div> | </div> | ||
<div align = "center"/> | <div align = "center"/> | ||
| − | <img class="img-responsive" style="width:60% align = "center" src="https://static.igem.org/mediawiki/2019/c/cb/T--Rice--Poopidyscoop.svg"/> | + | <img class="img-responsive" style="width:60%" align = "center" src="https://static.igem.org/mediawiki/2019/c/cb/T--Rice--Poopidyscoop.svg"/> |
</div> | </div> | ||
</div> | </div> | ||
Revision as of 03:06, 22 October 2019
Motivation
Existing RNA thermometers operate around 37°C and do not exhibit significant conformational changes between 25°C and 30°C. As most plants will die at 37°C, it was necessary to design thermometers that experienced conformational change between 25°C. We chose to design thermometers by finding optimal candidates using a genetic algorithm, as the complexity of RNA folding made it difficult to rationally design thermometers. As the figures below show,A. Thaliana grows best between 18-20°C and P. putdia is typically grown in lab at 30°C. These two facts combine help strengthen the need to design RNA thermometers that are optimized to melt at 30°C.
How RNA Thermometers Regulate Translation
In essence, RNA thermometers are a form of temperature dependent translational regulation. At low temperatures, there is a higher probability of more base pairs forming what is known as a "stem-loop structure". At higher temperatures, the thermometers "melt", meaning there is a decreased likelihood of base pairs forming.
