Team:Rice/Improve

Improving RNA Thermometer Design

We improved the part BBa_K11502, an RNA thermometer optimized for unfolding at 37 °C. By modifying the nucleotide sequence around the Shine-Dalgarno sequence, we were able to create a new part, BBa_K3247005, which we called RNA Thermometer NoChill-06, that obtains a much higher change in expression between 25 °C and 37 °C. Please look at the thermometer design page for more details about our thermometer design process. It's important to note that the shine dalgarno sequences used are slightly different. The Tu Delft's team used AGGAGG, while ours used AGGAGA. Also, take note of the differences in y axis.

Figure 3 : Fluorescence of the FourU kit thermometer from the TU Delft iGEM 2008 team at 25°C, 30°C and 37°C. This thermometer was optimized for 37°C according to their part registry page.

Figure 4: Florescence of our NoChill thermometer at 25°C, 30°C, and 37°C. This thermometer was optimized for 30°C.