Team:Rice/Contribution

Kit RNA Thermometer Characterization

We added quantitative experimental characterization data to the following parts: BBa_K115001, BBa_K115003, BBa_K115017. We looked at how expression of a fluorescent protein changed with temperature. Details of our procedure can be found on the experiments page.

Novel Part Contribution

We created new RNA thermometers and characterized these basic parts on their respective Part Main Pages. The parts we submitted on the Judging Form are BBa_K3247000 (NoChill_01), BBa_K3247001 (NoChill_02), BBa_K3247003 (NoChill_04). Other RNA thermometers we created and characterized include BBa_K3247002 (NoChill_03), BBa_K3247004 (NoChill_05), BBa_K3247005 (NoChill_06).

Individual thermometer fluorescence data

Thermometers showed increased fluorescence, which corresponds with an increase in mCherry production, with increased temperature. This is taking into account our positive control circuit, which contained BBa_B0034 for the RBS and not a RNA thermometer.