Kit RNA Thermometer Characterization
We added quantitative experimental characterization data to the following parts: BBa_K115001, BBa_K115003, BBa_K115017. We looked at how expression of a fluorescent protein changed with temperature. Details of our procedure can be found on the experiments page.
![](https://static.igem.org/mediawiki/2019/1/16/T--Rice--BBa115001.png
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![](https://static.igem.org/mediawiki/2019/f/fe/T--Rice--BBa115003.png
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![](https://static.igem.org/mediawiki/2019/1/16/T--Rice--BBa115017.png
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Novel Part Contribution
We created new RNA thermometers and characterized these basic parts on their respective Part Main Pages. The parts we submitted on the Judging Form are BBa_K3247000 (NoChill_01), BBa_K3247001 (NoChill_02), BBa_K3247003 (NoChill_04). Other RNA thermometers we created and characterized include BBa_K3247002 (NoChill_03), BBa_K3247004 (NoChill_05), BBa_K3247005 (NoChill_06).
Individual thermometer fluorescence data
![](https://static.igem.org/mediawiki/2019/3/39/T--Rice--Nochill1.png
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![](https://static.igem.org/mediawiki/2019/a/ab/T--Rice--nochill2.png
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![](https://static.igem.org/mediawiki/2019/f/f1/T--Rice--nochill3.png
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![](https://static.igem.org/mediawiki/2019/c/c2/T--Rice--Nochill4.png
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![](
https://static.igem.org/mediawiki/2019/c/c1/T--Rice--nochill5.png
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![](https://static.igem.org/mediawiki/2019/0/0e/T--Rice--Nochill6.png
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Thermometers showed increased fluorescence, which corresponds with an increase in mCherry production, with increased temperature. This is taking into account our positive control circuit, which contained BBa_B0034 for the RBS and not a RNA thermometer.
![](https://static.igem.org/mediawiki/2019/a/a9/T--Rice--BBaB0034.png
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![](https://static.igem.org/mediawiki/2019/1/11/T--Rice--3025v2.png
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![](https://static.igem.org/mediawiki/2019/6/6e/T--Rice--3725v2.png
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![](
https://static.igem.org/mediawiki/2019/0/0b/T--Rice--3730v2.png
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