Difference between revisions of "Team:Rice/Model"
| Line 151: | Line 151: | ||
| − | <div class="row" style = "background-color:# | + | <div class="row" style = "background-color:#9bcfa7!important;"> |
<h1 style = "background-color:#9bcfa7!important;"> Motivation <h1> | <h1 style = "background-color:#9bcfa7!important;"> Motivation <h1> | ||
<p>Existing RNA thermometers operate around 37°C and do not exhibit significant conformational changes between 25°C and 30°C. As most plants will die at 37°C, it was necessary to design thermometers that experienced conformational change between 25°C. We chose to design thermometers by finding optimal candidates using a genetic algorithm, as the complexity of RNA folding made it difficult to rationally design thermometers. As the figures below show,<i>A. Thaliana</i> grows best between 18-20°C and <i>P. putdia </i> is typically grown in lab at 30°C. These two facts combine help strengthen the need to design RNA thermometers that are optimized to melt at 30°C. | <p>Existing RNA thermometers operate around 37°C and do not exhibit significant conformational changes between 25°C and 30°C. As most plants will die at 37°C, it was necessary to design thermometers that experienced conformational change between 25°C. We chose to design thermometers by finding optimal candidates using a genetic algorithm, as the complexity of RNA folding made it difficult to rationally design thermometers. As the figures below show,<i>A. Thaliana</i> grows best between 18-20°C and <i>P. putdia </i> is typically grown in lab at 30°C. These two facts combine help strengthen the need to design RNA thermometers that are optimized to melt at 30°C. | ||
</p> | </p> | ||
| − | <div class="column" style="background-color:# | + | <div class="column" style="background-color:#9bcfa7!important;"> |
<img class="img-responsive" style="width:100%" src="https://static.igem.org/mediawiki/2019/e/e0/T--Rice--arabidopsisgrowingtemp.svg"/> | <img class="img-responsive" style="width:100%" src="https://static.igem.org/mediawiki/2019/e/e0/T--Rice--arabidopsisgrowingtemp.svg"/> | ||
</div> | </div> | ||
| − | <div class="column" style="background-color:# | + | <div class="column" style="background-color:#9bcfa7!important;"> |
<img class="img-responsive" style="width:100%" src="https://static.igem.org/mediawiki/2019/d/dd/T--Rice--putidagrowingtemp.svg"/> | <img class="img-responsive" style="width:100%" src="https://static.igem.org/mediawiki/2019/d/dd/T--Rice--putidagrowingtemp.svg"/> | ||
</div> | </div> | ||
Revision as of 02:53, 22 October 2019
Motivation
Existing RNA thermometers operate around 37°C and do not exhibit significant conformational changes between 25°C and 30°C. As most plants will die at 37°C, it was necessary to design thermometers that experienced conformational change between 25°C. We chose to design thermometers by finding optimal candidates using a genetic algorithm, as the complexity of RNA folding made it difficult to rationally design thermometers. As the figures below show,A. Thaliana grows best between 18-20°C and P. putdia is typically grown in lab at 30°C. These two facts combine help strengthen the need to design RNA thermometers that are optimized to melt at 30°C.
How RNA Thermometers Regulate Translation
In essence, RNA thermometers are a form of temperature dependent translational regulation. At low temperatures, there is a higher probability of more base pairs forming what is known as a "stem-loop structure". At higher temperatures, the thermometers "melt", meaning there is a decreased likelihood of base pairs forming.
