We use E.coli in our experiment. The strains are DH5-alpha, BL21(DE3),JM109 delta MreB.

Normal E.coli will not pose threat to the environment or people. However, if the E.coli carrying antibiotic resistance gene escapes, the tolerance strain or this tolerance gene might spread.

Normal E.coli will not pose threat to the environment or people. However, if the E.coli carrying antibiotic resistance gene escapes, the tolerance strain or this tolerance gene might spread.

The unmodified E.coli will not pose threat to environment and people. However, if the E.coli with plasmids that carry the antibiotic resistance gene escape, the tolerance strain or this tolerance gene might spread.

We've already uploaded the spreadsheet.

We will use E.coli DH5alpha and BL21 as our chasis to develop a light induced phase separation system to control biochemical reactions inside the bacteria. SIM, SUMO and FUS will be use as phase separation elements. Cry2 and CIB1 will be fused to phase separation elements to induce phase separation under light stimulation. 3HSB is a hexamer used to verify the ability of phase separation element to aggregate under induction.

We will use pointy objects to plant bacteria onto the medium, which may hurt hands without gloves. We will use autoclave to sterilize facilities and reagents, which may cause injury if opened under high temperature.

Only in the lab.

The main risk of this application is the escape of transgenic E. coli. It would introduce synthetic genetic material to the environment, causing unpredictable gene transition in nature, including resistance for certain antibiotics.

The Tsinghua iGEM lab is part of the Center for Laboratory Training in Life Sciences in Tsinghua University. Lab instructor Li will oversee our experiments. Also we will work with instructors from Chen Lab, the laboratory of our Primary PI that specializes in microbiology research. Last but not least, we have a security commmittee evaluating the risks that might happen in our experiments.

http://www.gov.cn/zhengce/content/2008-03/28/content_6264.htm http://www.tsinghua.edu.cn/publish/sbc/202/2010/20101208153448737847778/20101208153448737847778_.html

Yes, we have already received safety training and we have already received security training.

• Lab access and rules (including appropriate clothing, eating and drinking, etc.
• Responsible individuals (such as lab or departmental specialist or institutional biosafety officer)
• Differences between biosafety levels
• Biosafety equipment (such as biosafety cabinets)
• Good microbial technique (such as lab practices)
• Disinfection and sterilization
• Emergency procedures
• Transport rules
• Physical biosecurity
• Personnel biosecurity
• Dual-use and experiments of concern
• Data biosecurity
• Chemicals, fire and electrical safety

Open bench, Biosafety cabinet and nikon A1RMP multiphoton microscopy (A15000007)

Level 1

• Accident reporting (measures to record any accidents)
• Personal Protective Equipment (including lab coats, gloves, eye protection, etc)
• An inventory control system (measures to track who has what materials and where they are)
• Access controls (measures to control who can access your work spaces, or where materials are kept)
• Medical surveillance (measures to find out if you get sick because of something you were using)
• Waste management system (measures to make sure waste is not hazardous before it leaves your institution)
• Special procedures or protocols that address safety or security

We will not use pathogenic strains of E. coli. Experiments will only be conducted when gloves and other protections are worn properly. Wastes containing living bacteria or harmful reagents will be sterilized before being disposed, or sent to qualified institution for disposal. Only iGEMers have can access our lab to carry out experiments. We also have security rules which all iGEMers will obey. The rules are confirmed by lab instructor and all iGEMers.

No.

No.

No.

No.

No.

No.

We think this safety form covers almost all risks we have thought about.