Difference between revisions of "Team:SCUT China/Composite Part"

 
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<p style="margin:30px; color:#FFF;text-align:justify"><span style="font-size:24px;line-height:150%;text-align:center;"><strong> <br>Best Composite Part<br><br></strong></span>
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<span style="font-size:20px;line-height:150%;text-align:justify"> We have built our acid tolerant working part named <a href="http://parts.igem.org/Part:BBa_K3100140">BBa_K3100140</a> which insert different Toehold switchs before the acid tolerant genes to activate their expression in response to cognate RNAs (Trigger RNAs) with arbitrary sequences. The acid tolerant working part play an important role in VerProS system which can fine regulation of 4 acid tolerant factor. After screening, we can obtain the strongest acid-tolerant strain.<br>
  
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First, we construct the 10 plasmids by gene synthesis or Gibson Assembly.
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                              <center><img src="https://static.igem.org/mediawiki/2019/e/e6/T--SCUT_China--Composite_1.jpg" style="text-align:center;width:600px;"></center><br><br>
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                              <p style="font-size:20px;line-height:150%;text-align:center; color:#FFF">Fig.1 : construct the 10 plasmids by gene synthesis or Gibson Assembly</p>
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<p style="margin:30px; color:#FFF;text-align:justify"><span style="font-size:20px;line-height:150%;text-align:justify;"><br>Second, use the 10 plasmids to construct acid tolerant working part by Golden Gate Assembly.<br></span>
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                              <center><img src="https://static.igem.org/mediawiki/2019/5/5a/T--SCUT_China--Composite_2.jpg" style="text-align:center;width:600px;"></center><br><br>
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                              <p style="font-size:20px;line-height:150%;text-align:center; color:#FFF">Fig 2: construct acid tolerant working part by Golden Gate Assembly</p>
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<h1>Composite Parts</h1>
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<h3>Note</h3>
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<p>This page should list all the composite parts your team has made during your project and include direct links to your Parts main pages on the Registry. <b>You must add all characterization information for your parts on Parts Main Page on the Registry.</b> You should <b>not</b> put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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<h3>Best Composite Part Special Prize</h3>
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<p>To be eligible for this award, this part <b>must be well documented on the part's Main Page on the Registry</b>. If you have a part you wish to nominate your team for this <a href="https://2019.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2019.igem.org/Judging/Judging_Form">judging form</a> and delete the alert box at the top of this page.
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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Latest revision as of 23:04, 21 October 2019

Ruby - Responsive Corporate Tempalte


Best Composite Part

 We have built our acid tolerant working part named BBa_K3100140 which insert different Toehold switchs before the acid tolerant genes to activate their expression in response to cognate RNAs (Trigger RNAs) with arbitrary sequences. The acid tolerant working part play an important role in VerProS system which can fine regulation of 4 acid tolerant factor. After screening, we can obtain the strongest acid-tolerant strain.
First, we construct the 10 plasmids by gene synthesis or Gibson Assembly.




Fig.1 : construct the 10 plasmids by gene synthesis or Gibson Assembly


Second, use the 10 plasmids to construct acid tolerant working part by Golden Gate Assembly.




Fig 2: construct acid tolerant working part by Golden Gate Assembly