Team:SCUT China/Attributions

Throughout the work on our project we learned that iGEM is not all about science, but also about the people behind it. This entire project and its many successes would not have been possible without the support of so many individuals. We wish to extend our deepest appreciation to everyone who has contributed to the project for the great support and help in this project.

 

Team work

Each and every team member has shown tremendous effort for the contribution to our project. All student team members performed outstanding work and showed interest and dedication to the project. As we are a rather small team, we did not have a strict division of roles and many times we worked all together to accomplish certain parts of our project.

 

Team members	Attributions
Jerome	Responsible for Human Practices and collaboration, Experiments
Jane	Construction of the parts, Experiments
Heulwen	Design of all deliverables, Experiments
Bing	Modeling, Process data, Experiments
Michelle	Write some parts of the wiki text, Experiments
Avery	Collate and translate the protocol, Experiments
Skye	Design of project, Experiments
Chen Hongqian	Design of wiki
Dong Yuzhu	Design of wiki
Xu Kuan	Modeling
Zhang Tianyi	Design of inbetweening
Li Xuchun	Design of inbetweening
Li Xiafang	Design of questionnaire
Wang Ying	Design of animation

 

 

Lab Support

We would like to express our gratitude to all the researchers that assisted us and provided their scientific knowledge. We were honored to have the opportunity to learn so much from them.

Laboratory support

Professor Zhanglin Lin, from the School of Biology and Biological Engineering of South China University of Technology, for providing us with the laboratory and allowing us to use his lab’s materials when completing the study throughout the course of our project.

 

Experts support

Dr. Tingting Wang, for designing our plasmid constructions as well as the troubleshooting with the Golden Gate Technology to DNA assembling.
Dr. Ping Chen, for guiding us on conducting multi-sequence alignment analysis.

 

Advisors support

Support from the graduate students in Professor Zhanglin Lin's laboratory is crucial for our framework validation, early troubleshooting, equipment operation and our daily operations.
Graduate students Lan He, Danni Lu and Huanhuan Li, forguiding our molecular cloning experiments.
Graduate student Qingxiu Hu, for guiding us in Western blot.

 

 

Project support

Negative selection plasmid:

The laboratory of Tsinghua University, for providing us with the plasmid pT416 containing ccdB gene.

 

T7 library:

Before we improve T7 promoters, we used ten T7 promoter sequences of different strength as the preliminary experimental attempt in our project found at:
Supplementary Information of Balancing gene expression without library construction via a reusable sRNA pool.

 

Golden Gate Assembly:

The Golden Gate DNA shuffling strategy of our project found at:
Carola Engler., Ramona Gruetzner., Romy Kandzia, Sylvestre Marillonnet(2009). Golden Gate Shuffling: A One-Pot DNA Shuffling Method Based on Type IIs Restriction Enzymes.

 

Toehold Switch:

Our project is based on the research method of Alexander A. Green to design and verify the biological switch. The sequence of Toehold Switch elements and the method of element characterization found at:
Alexander A. Green, Pamela A. Silver,James J. Collins, and Peng Yin(2014). Toehold Switches_ De-Novo-Designed Regulators of Gene Expression.

 

pACYC184-J23100-RFP:

Graduate student Xurong Yao, for providing us with the plasmid pACYC184-J23100-RFP.

 

BioS Club:

BioS is a club founded by students who love synthetic biology. Every class of members will receive training on biology knowledge from the club. Almost all the members of iGEM SCUT_China team are members of BioS club.

 

Wiki:

Sarasvati Media Studio is a media studio created by our team to better complete the visual design of our project. They help our wiki pattern design. Although they were not able to go to Boston, they steered the team’s vision for graphic design and communication strategies. This would not have been possible without their amazing skill sets.

 

 

Financial support
Trip support

The International Office and the academic affairs office of the School of Biology and Biological Engineering of SCUT provided crucial financial support for our trip to Giant Jamboree Boston.

 

Commercial services

Sangon Biotech Co., Ltd. and TSINGKE Co., Ltd. helped Team SCUT_China do the synthesis.

 

 

Human Practices support

Shanbin Huang, thanks for the guidance on the basic knowledge of sociology;
Zhen Yi, thanks for his help in social survey subjects.

 

Contributors	Thanks for
Lab Support
Zhanglin Lin	Providing the laboratory and lab’s materials
Tingting Wang	Designing plasmid constructs
Lan He, Huanhuan Li, Dani Lu	Guiding molecular cloning experiments
Qingxiu Hu	Guiding Western blot
Project Support
Tsinghua University	Providing the plasmid pT416-ccdB
Amar Ghodasara and Christopher A. Voigt.	Providing T7 promoter sequences
Alexander A. Green, Pamela A. Silver, et al	Providing the sequence of Toehold Switch
Xurong Yao	Providing the plasmid pACYC184-J23100-RFP
Bios club	Training on biology knowledge
Sarasvati Media Studio	Helping wiki pattern design
Financial support
The International Office and the academic affairs office of SCUT	Trip support
Sangon Biotech Co., Ltd. and TSINGKE Co., Ltd.	Gene synthesis
Human Practices support
Shanbin Huang	Guiding on the basic knowledge of sociology
Zhen Yi	Guiding on social survey subjects

 

Thank you for each of you for making our project more perfect and enabling us to share our achievements and joy with more people around the world. You make us better iGEMers.