Difference between revisions of "Team:Marburg/test joana"

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         P A R T S O V E R V I E W
 
         P A R T S O V E R V I E W
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      <hr class="line">
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      <img src="https://static.igem.org/mediawiki/2019/a/ac/T--Marburg--logo.svg"
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        class="logo"
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        alt="Syntex Logo">
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    <div style="margin-top: 10vh;">
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      <section class="section">
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        <h1 class="title">A.P.P Automated Purfication Protocolment</h1>
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        <p style="text-align: justify;">
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              BLABLA.<br>
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                <br>
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        </p>
 
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                             <br>
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            <figure align=center>
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                <img style="height: 500px; width: 300px"
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                    src="https://static.igem.org/mediawiki/2019/b/bb/T--Marburg--opentrons_magnetic_module.JPG"
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                    alt="OT-2 left">
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                <img style="height: 500px; width: 300px"
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                    src="https://static.igem.org/mediawiki/2019/3/30/T--Marburg--opentrons_shaker.JPG" alt="OT-2 right">
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                <figcaption style="max-width: 1400px">
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                    Fig.2 - Single-Channel pipette, magnetic module and shaker in action while performing the plasmid
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                    purification.
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                </figcaption>
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            </figure>
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            <br>
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            <p>
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                Putting the pieces together, we were able to translate the manual plasmid purification protocol provided by Nans
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                Bodet into an Opentrons protocol, being the very first of its kind. We pioneered a workflow for up to six
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                samples with the p300 Single-Channel Electronic Pipette and a scaled-up version for up to 48 samples with the
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                p300 8-Channel Electronic Pipette without having to intervene even once. This scalability provides important
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                flexibility for various kinds of experiments.<br>
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                <br>
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                In our process of developing and running the protocol we determined some problems on increasing the yield of our
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                plasmids. There was a large number of parameters that could be varied, changing the final concentration of the
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                plasmids. For example, we realized that the duration of lysis is paramount for the yield and success of plasmid
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                purification. Over-lysis will lead to a decrease in plasmid yield, whereas under-lysis will induce clumping of
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                magnetic beads; thus failing the experiment. After a whole heap of plasmid purifications we managed to identify
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                the most relevant parameters and improve the protocol in the best way possible.<br>
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                <br>
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            </p>
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            <figure align=center>
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                <img style="height: 700px; width: 600px"
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                    src="https://static.igem.org/mediawiki/2019/e/ea/T--Marburg--SingleChannelSetup.png" alt="OT-Layout left">
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                <img style="height: 700px; width: 600px"
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                    src="https://static.igem.org/mediawiki/2019/d/df/T--Marburg--8channelSetup.png" alt="OT-Layout right">
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                <figcaption style="max-width: 1400px">
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                    Fig.3 - Final setup for the automated plasmid purification workflows in the OT-2. The left picture shows the
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                    setup for the single channel workflow, the right picture for the 8-channel workflow.
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                </figcaption>
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            </figure>
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            <video src="https://static.igem.org/mediawiki/2019/c/c4/T--Marburg--PlasmidPurificationMarburg.mp4" controls
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                poster="vorschaubild.jpg"></video>
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            <br>
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Revision as of 22:59, 21 October 2019

P A R T S O V E R V I E W

A.P.P Automated Purfication Protocolment

BLABLA.

Parts Overview



With our additional created parts, we expanded the current Marburg Collection.


Best Basic Part

1-2 sentence abstract


Best Composite Part

1-2 sentence abstract


Marburg Collection 2.0

1-2 sentence abstract