Difference between revisions of "Team:Fudan-TSI/Parts"
| Line 1,826: | Line 1,826: | ||
<div class="col col-lg-12"> | <div class="col col-lg-12"> | ||
This year Team Fudan-TSI presents you with a toolbox for our <i>in vivo</i> sequence-specific continuous mutagenesis system (R-Evolution). This toolbox includes promoters, RBSs, terminators, CDSs and other regulatory parts which undergo careful design and optimization. The Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT), which is new to the registry, and Cre Recombinase are the most important components of our system for which we have put emphasis on the construction and characterization of these two parts. Also, our improved lac repressor protein is fully equipped for regulating protein expression.<br /><br /> | This year Team Fudan-TSI presents you with a toolbox for our <i>in vivo</i> sequence-specific continuous mutagenesis system (R-Evolution). This toolbox includes promoters, RBSs, terminators, CDSs and other regulatory parts which undergo careful design and optimization. The Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT), which is new to the registry, and Cre Recombinase are the most important components of our system for which we have put emphasis on the construction and characterization of these two parts. Also, our improved lac repressor protein is fully equipped for regulating protein expression.<br /><br /> | ||
| − | Specifically, the parts that we are presenting this year | + | Specifically, the parts that we are presenting this year include but are not limited to these following features: <br/><br/> |
1) We attached Cre with different degradation tags, which could be used according to user’s interest to achieve optimal recombination efficiency.<br /> | 1) We attached Cre with different degradation tags, which could be used according to user’s interest to achieve optimal recombination efficiency.<br /> | ||
2) We placed MMLV-RT under different IPTG-inducible promoters, which provides a range of different steady-state expression levels for various experimental purposes.<br /> | 2) We placed MMLV-RT under different IPTG-inducible promoters, which provides a range of different steady-state expression levels for various experimental purposes.<br /> | ||
Revision as of 22:55, 21 October 2019
Parts Overview
This year Team Fudan-TSI presents you with a toolbox for our in vivo sequence-specific continuous mutagenesis system (R-Evolution). This toolbox includes promoters, RBSs, terminators, CDSs and other regulatory parts which undergo careful design and optimization. The Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT), which is new to the registry, and Cre Recombinase are the most important components of our system for which we have put emphasis on the construction and characterization of these two parts. Also, our improved lac repressor protein is fully equipped for regulating protein expression.
Specifically, the parts that we are presenting this year include but are not limited to these following features:
1) We attached Cre with different degradation tags, which could be used according to user’s interest to achieve optimal recombination efficiency.
2) We placed MMLV-RT under different IPTG-inducible promoters, which provides a range of different steady-state expression levels for various experimental purposes.
3) We included additional regulatory sequences (e.g. native primer binding site and poly-purine tract) required for the initiation and completion of reverse transcription.
4) To eliminate self-excision of Cre and promote recombination efficiency, we included a set of incompatible loxP sites.
5) We provide testing plasmids for system verification and optimization.
In summary, our part collection provides a complete set for assembly, test, and optimization of continuous mutagenesis in different prokaryotic hosts.
Specifically, the parts that we are presenting this year include but are not limited to these following features:
1) We attached Cre with different degradation tags, which could be used according to user’s interest to achieve optimal recombination efficiency.
2) We placed MMLV-RT under different IPTG-inducible promoters, which provides a range of different steady-state expression levels for various experimental purposes.
3) We included additional regulatory sequences (e.g. native primer binding site and poly-purine tract) required for the initiation and completion of reverse transcription.
4) To eliminate self-excision of Cre and promote recombination efficiency, we included a set of incompatible loxP sites.
5) We provide testing plasmids for system verification and optimization.
In summary, our part collection provides a complete set for assembly, test, and optimization of continuous mutagenesis in different prokaryotic hosts.