Nicole Guo (Talk | contribs) |
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<h2 class="editContent">The Methanol Converter</h2> | <h2 class="editContent">The Methanol Converter</h2> | ||
<h3 class="editContent"><em>Pichia pastoris GS115: From methanol to medicine, how to do it better?</em></h3> | <h3 class="editContent"><em>Pichia pastoris GS115: From methanol to medicine, how to do it better?</em></h3> | ||
− | <p>Methanol is a major byproduct of the coal industry, and engineered <em>Pichia pastoris GS115</em> can convert methanol into medical compounds such as the insulin precursor and lovastatin. However, this pathway results in significant oxygen consumption and heat generation, limiting its industrial applications. We improved the methanol conversion rate in <em>P. pastoris</em> by rewiring its homogeneous regulatory circuits to up-regulate the expression of alcohol oxidase 1, the protein that allows it to metabolize methanol. Improving the conversion efficiency lowers heat generation as all heat comes from methanol metabolism. Through multiple rounds of experiments, we acquired strains that are able to yield an up to 20% increase in methanol conversion rate compared to the wildtype | + | <p>Methanol is a major byproduct of the coal industry, and engineered <em>Pichia pastoris GS115</em> can convert methanol into medical compounds such as the insulin precursor and lovastatin. However, this pathway results in significant oxygen consumption and heat generation, limiting its industrial applications. We improved the methanol conversion rate in <em>P. pastoris</em> by rewiring its homogeneous regulatory circuits to up-regulate the expression of alcohol oxidase 1, the protein that allows it to metabolize methanol. Improving the conversion efficiency lowers heat generation as all heat comes from methanol metabolism. Through multiple rounds of experiments, we acquired strains that are able to yield an up to 20% increase in methanol conversion rate compared to the wildtype. </p> |
<a href="https://2019.igem.org/Team:ShanghaiFLS_China/Design">Design</a> | <a href="https://2019.igem.org/Team:ShanghaiFLS_China/Design">Design</a> | ||
<a href="https://2019.igem.org/Team:ShanghaiFLS_China/Experiments">Experiment</a> | <a href="https://2019.igem.org/Team:ShanghaiFLS_China/Experiments">Experiment</a> |
Revision as of 07:08, 21 October 2019
About us
We are a diverse group of high school students from China, studying in Beijing, Tianjin, the United States or Switzerland, as well as in Shanghai Foreign Language School. We have different hobbies, ambitions, and views towards bioengineering. We also have different roles to play this fascinating project. For more information about us, please check the links below:
Team Members
Attributions