Nicole Guo (Talk | contribs) m |
Nicole Guo (Talk | contribs) |
||
Line 105: | Line 105: | ||
<h3 class="editContent"><em>Pichia Pastoris</em> GS115:</h3> | <h3 class="editContent"><em>Pichia Pastoris</em> GS115:</h3> | ||
<h2 class="editContent">The Methanol Converter</h2> | <h2 class="editContent">The Methanol Converter</h2> | ||
− | <h3 class="editContent"><em>From methanol to medicine, how to do it better?</em> | + | <h3 class="editContent"><em>From methanol to medicine, how to do it better?</em></h3> |
<p>Methanol is a major byproduct of the coal industry. Engineered <em>Pichia pastoris</em> GS115 can convert methanol into medical compounds such as the insulin precursor and lovastatin. However, this pathway results in significant oxygen consumption and heat generation, limiting its industrial applications. We improved the methanol conversion rate in <em>P. pastoris</em> by rewiring its homogeneous circuits expressing the transcription factors that would up-regulate the expression of alcohol oxidase 1, the protein that allows it to metabolize methanol. Through multiple rounds of experiments, we acquired strains that are able to yield an up to 20% increase in total production per unit methanol compared to the wildtype. In other words, these strains generate less heat producing the same amount of produce.</p> | <p>Methanol is a major byproduct of the coal industry. Engineered <em>Pichia pastoris</em> GS115 can convert methanol into medical compounds such as the insulin precursor and lovastatin. However, this pathway results in significant oxygen consumption and heat generation, limiting its industrial applications. We improved the methanol conversion rate in <em>P. pastoris</em> by rewiring its homogeneous circuits expressing the transcription factors that would up-regulate the expression of alcohol oxidase 1, the protein that allows it to metabolize methanol. Through multiple rounds of experiments, we acquired strains that are able to yield an up to 20% increase in total production per unit methanol compared to the wildtype. In other words, these strains generate less heat producing the same amount of produce.</p> | ||
<a href="https://2019.igem.org/Team:ShanghaiFLS_China/Design">Design</a> | <a href="https://2019.igem.org/Team:ShanghaiFLS_China/Design">Design</a> |
Revision as of 07:00, 21 October 2019
About us
We are a diverse group of high school students from China, studying in Beijing, Tianjin, the United States or Switzerland, as well as in Shanghai Foreign Language School. We have different hobbies, ambitions, and views towards bioengineering. We also have different roles to play this fascinating project. For more information about us, please check the links below:
Team Members
Attributions