Difference between revisions of "Team:Rice/Design"
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<p> We had originally sought to use a vector that had RK2 with <i>araC</i> for both the thermometer and enzyme testing. RK2 has been reported to function well in <i>P. putida</i>, but after 2 months of trying, we performed control experiments of various different combinations of inducer and origins, and the only one that consistently would not clone was RK2 and <i>araC</i>. Because of this, we chose the broad host origin RSF1010. LacI was used temporarily as we were trouble shooting <i>araC</i>, but we decided to keep it in the system in order to be consistent.</p> | <p> We had originally sought to use a vector that had RK2 with <i>araC</i> for both the thermometer and enzyme testing. RK2 has been reported to function well in <i>P. putida</i>, but after 2 months of trying, we performed control experiments of various different combinations of inducer and origins, and the only one that consistently would not clone was RK2 and <i>araC</i>. Because of this, we chose the broad host origin RSF1010. LacI was used temporarily as we were trouble shooting <i>araC</i>, but we decided to keep it in the system in order to be consistent.</p> | ||
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<h1 style = "background-color:#ff6201!important;">RNA Thermometer Assembly </h1> | <h1 style = "background-color:#ff6201!important;">RNA Thermometer Assembly </h1> | ||
Revision as of 20:34, 20 October 2019
Cloning method
Our type IIS assembly method
Circuit Design
Construct for testing the enzymes
acdS Cassette
acdS encodes for 1-aminocyclopropane-1-carboxylate (ACC) deaminase synthase. ACC deaminase helps reduce the overall concentrations of ethylene in the plant by converting its precursor, ACC, into ethylene and ammonium.
iaaMH Cassette
Both iaaM and iaaH function to synthesize indole-3-acetic acid (IAA) from tryptophan. iaaM encodes for tryptophan mono oxygenase and iaaH encodes for indoleacetamide hydrolase.
otsBA Cassette
Both otsB and otsA function to synthesize trehalose synthase from glucose molecules. otsA encodes for trehalose-6-phosphate synthase and otsB encodes for trehalose-6-phosphate phosphatase.
