Difference between revisions of "Team:Tsinghua/Team"

Line 355: Line 355:
 
              
 
              
 
             <div class="container">
 
             <div class="container">
 +
                <div class="row">
 +
        <div class="col-lg-4">
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/0/0b/T--Tsinghua--cgq.jpg" alt="" />
 +
                <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100">
 +
                   
 +
                </div>
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100">
 +
                        <h3 style="color: rgba(255,255,255,1.00)">Team Instructor</h3>
 +
                    </div>
 +
                </div>
 
                  
 
                  
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Guoqiang Chen</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/9/95/T--Tsinghua--szr.jpg" alt="" />
 +
                <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100">
 +
                    <div class="hoverbox-content p-4">
 +
                        <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100">
 +
                            <h3 style="color: rgba(255,255,255,1.00)">Team Instructor</h3>
 +
                        </div>
 +
                    </div>
 +
                </div>
 +
               
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Zhirong Sun</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/f/f7/T--Tsinghua--lp.jpg" alt="" />
 +
               
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Team Instructor</h3></div>
 +
                </div>
 +
               
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Peng Li</center></h4>
 +
        </div>
 +
    </div>
 +
    <div class="row">
 +
        <div class="col-lg-4">
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/2/25/T--Tsinghua--wx.jpg" alt="" />
 +
                <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100">
 +
                   
 +
                </div>
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Team Instructor</h3></div>
 +
                </div>
 +
               
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Xuan Wang</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/2/2f/T--Tsinghua--yla.jpg" alt="" />
 +
                <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100">
 +
                    <div class="hoverbox-content p-4">
 +
                        <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div>
 +
                    </div>
 +
                </div>
 +
               
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Liang Yao</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/e/e1/T--Tsinghua--hjn.jpg" alt="" />
 +
               
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div>
 +
                </div>
 +
               
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Jianing Han</center></h4>
 +
        </div>
 +
    </div>
 +
    <div class="row">
 +
        <div class="col-lg-4">
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/e/e8/T--Tsinghua--cxy.jpg" alt="" />
 +
                <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100">
 +
                   
 +
                </div>
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div>
 +
                </div>
 +
               
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Xinyi Chen</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/d/dd/T--Tsinghua--gxf.jpg" alt="" />
 +
                <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100">
 +
                    <div class="hoverbox-content p-4">
 +
                        <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div>
 +
                    </div>
 +
                </div>
 +
               
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Xiaofei Ge</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/f/fc/T--Tsinghua--yq.jpg" alt="" />
 +
               
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div>
 +
                </div>
 +
               
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Qi Yan</center></h4>
 +
        </div>
 +
    </div>
 +
    <div class="row">
 +
        <div class="col-lg-4">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/9/9e/T--Tsinghua--htz.jpg" alt="" />
 +
                <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100">
 +
                   
 +
                </div>
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Tianze Huang</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/a/a5/T--Tsinghua--wtx.jpg" alt="" />
 +
                <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100">
 +
                    <div class="hoverbox-content p-4">
 +
                        <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Tingxuan Wang</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/6/63/T--Tsinghua--hyj.jpg" alt="" />
 +
               
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Advisor</h3></div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Yuejun Hao</center></h4>
 +
        </div>
 +
    </div>
 +
    <div class="row">
 +
        <div class="col-lg-4">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/c/c9/T--Tsinghua--hzk.jpg" alt="" />
 +
                <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100">
 +
                   
 +
                </div>
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Team Leader / Wet Lab</h3></div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Zikang Huang</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/5/57/T--Tsinghua--ccy.jpg" alt="" />
 +
                <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100">
 +
                    <div class="hoverbox-content p-4">
 +
                        <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Team Leader / Finance</h3></div>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Chunyu Chen</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/1/10/T--Tsinghua--lhr.jpg" alt="" />
 +
               
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Wet Lab</h3></div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Hongrui Liu</center></h4>
 +
        </div>
 +
    </div>
 +
    <div class="row">
 +
        <div class="col-lg-4">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/1/1a/T--Tsinghua--cbw.jpg" alt="" />
 +
                <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100">
 +
                   
 +
                </div>
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Modeling / Wiki</h3></div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Bowen Chen</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/5/57/T--Tsinghua--fst.jpg" alt="" />
 +
                <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100">
 +
                    <div class="hoverbox-content p-4">
 +
                        <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Wet Lab</h3></div>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Site Feng</center></h4><strong></strong>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/e/e1/T--Tsinghua--fm.jpg" alt="" />
 +
               
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Modeling</h3></div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Meng Fang</center></h4>
 +
        </div>
 +
    </div>
 +
    <div class="row">
 +
        <div class="col-lg-4">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/5/58/T--Tsinghua--fjl.jpg" alt="" />
 +
                <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100">
 +
                   
 +
                </div>
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Wiki / Photography</h3></div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Jiale Fu</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/b/b7/T--Tsinghua--zzh.jpg" alt="" />
 +
                <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100">
 +
                    <div class="hoverbox-content p-4">
 +
                        <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Design / Poster</h3></div>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Zihan Zhai</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/c/cd/T--Tsinghua--qch.jpg" alt="" />
 +
               
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Wet Lab</h3></div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Chuheng Qing</center></h4>
 +
        </div>
 +
    </div>
 +
    <div class="row">
 +
        <div class="col-lg-4">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/6/6a/T--Tsinghua--slz.jpg" alt="" />
 +
                <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100">
 +
                   
 +
                </div>
 +
                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"> <h3 style="color: rgba(255,255,255,1.00)">Wet Lab</h3></div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Lize Sun</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/6/6b/T--Tsinghua--lgz.jpg" alt="" />
 +
                <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100">
 +
                    <div class="hoverbox-content p-4">
 +
                        <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Wet Lab</h3></div>
 +
                    </div>
 +
                </div>
 +
            </div>
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            <h4 style="color: rgba(7,18,48,1.00)"><center>Genzhe Lu</center></h4>
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        </div>
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        <div class="col-lg-4 mt-4 mt-lg-0">
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            <div class="hoverbox">
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                <img src="https://static.igem.org/mediawiki/2019/e/e6/T--Tsinghua--gj.jpg" alt="" />
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                <div class="hoverbox-content p-4">
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                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Wet Lab</h3></div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Ji Gao</center></h4>
 +
        </div>
 +
    </div>
 +
    <div class="row">
 +
        <div class="col-lg-4">
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            <div class="hoverbox">
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                <img src="https://static.igem.org/mediawiki/2019/5/56/T--Tsinghua--ljy.jpg" alt="" />
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                <div class="hoverbox-primary-content as-hoverbox-content d-flex align-items-center justify-content-center h-100">
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                </div>
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                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100">
 +
                        <h3 style="color: rgba(255,255,255,1.00)">Human Practice</h3>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Jiayi Li</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/7/79/T--Tsinghua--zlq.jpg" alt="" />
 +
                <div class="hoverbox-primary-content d-flex align-items-center justify-content-center h-100">
 +
                    <div class="hoverbox-content p-4">
 +
                        <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Logistic Support</h3></div>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Liqun Zhou</center></h4>
 +
        </div>
 +
        <div class="col-lg-4 mt-4 mt-lg-0">
 +
           
 +
            <div class="hoverbox">
 +
                <img src="https://static.igem.org/mediawiki/2019/e/e2/T--Tsinghua--logo_black.jpg" alt="" />
 +
               
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                <div class="hoverbox-content p-4">
 +
                    <div class="hoverbox-bg d-flex align-items-center justify-content-center h-100"><h3 style="color: rgba(255,255,255,1.00)">Central Part</h3></div>
 +
                </div>
 +
            </div>
 +
            <h4 style="color: rgba(7,18,48,1.00)"><center>Phase</center></h4>
 +
        </div>
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    </div>
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    <div class="row my-4 my-lg-6">
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            <img src="https://static.igem.org/mediawiki/2019/8/8b/T--Tsinghua--hy2.jpg" alt="" />
 +
            <h2><center>Meet Our Team Here!</center></h2>
 +
        </div>
 +
       
 +
    </div>
 +
   
 
             </div>
 
             </div>
 
         </section>
 
         </section>

Revision as of 19:34, 20 October 2019

iGEM Tsinghua

Meet Our Team

In order to precisely control the velocity of biochemical reactions, we applied phase separation system coupled with light induction in E.coli.

Phase separation system

We constructed the first artificial phase separation system ever in prokaryotes. These droplets in E.coli exhibit similar spherical shape and high fluidity as they were in eukaryotes. By creating membraneless organelles in a cell, phases could realize the redistribution of various targets, including proteins and small organic molecules.

Photo-activated switch

We use laser as a switch to control our system in a high spatial-temporal resolution. Within a few seconds, target proteins would redistribute into existing phases. After incubated in dark for several minutes, targets can reverse to previous smear state.

Modelling our system

We modelled the phase separation system and coupled enzymatic reactions using a differential equation model and a collision model of chemical reactions. The model conceptually explained how phase separation would regulate downstream reactions, and provided insights for enzyme concentration and phase separation system selection. In addition, an interactive interface based on the model is presented on our wiki.

Downstream reactions

Enzymes of proper size could be recruited into phases by using photo-activated switch. Selecting substrates of proper solubility according to our model, we realized the regulation of enzymatic reactions.

Improvement

Enrichment of CRY2 in phase include both homo-aggregation of itself and hetero-aggregation of CRY2 and CIB1. By introducing point mutation to C terminal of CRY2, we attenuate homo-aggregation of CRY2 in dark, increasing the threshold of light induction. This improvement may also be helpful for teams who want to modify the sensitivity of their light-controlled information-processing system.

More possibilities

Upon introducing this powerful tool in E.coli, there are much more prosperous applications waiting to be developed. We propose that by isolating specific material in phase, a uniform E.coli colony could grow into a society with cell differentiation.

Hardware

Depending on the manner of reversible amplification in phase, specific proteins can be used to create a biological register. Responding to laser stimulation, the distribution of fluorescence proteins would change, resulting in the variation in FRET signal, which can be detected by fluorescence reader.

Team Instructor

Guoqiang Chen

Team Instructor

Zhirong Sun

Team Instructor

Peng Li

Team Instructor

Xuan Wang

Advisor

Liang Yao

Advisor

Jianing Han

Advisor

Xinyi Chen

Advisor

Xiaofei Ge

Advisor

Qi Yan

Advisor

Tianze Huang

Advisor

Tingxuan Wang

Advisor

Yuejun Hao

Team Leader / Wet Lab

Zikang Huang

Team Leader / Finance

Chunyu Chen

Wet Lab

Hongrui Liu

Modeling / Wiki

Bowen Chen

Wet Lab

Site Feng

Modeling

Meng Fang

Wiki / Photography

Jiale Fu

Design / Poster

Zihan Zhai

Wet Lab

Chuheng Qing

Wet Lab

Lize Sun

Wet Lab

Genzhe Lu

Wet Lab

Ji Gao

Human Practice

Jiayi Li

Logistic Support

Liqun Zhou

Central Part

Phase

Meet Our Team Here!

PhASE

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