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− | Previous studies have reported that the interactions between tRNA primer and mRNA template as well as the reverse transcriptase are crucial in directing subsequent cDNA synthesis <a href="Ref1">(James E. Dahlberg et al.)</a>. Specifically, according to the model for reverse transcription proposed by <a href="Ref2">Kulpa et al.</a>, reverse transcription includes 5 steps (<a href="Fig1">Fig. 1</a>), in which the annealing of tRNA primer to the primer binding site (PBS) region on mRNA template is crucial for the synthesis of minus strand strong stop DNA (–ssDNA) and the following cDNA synthesis process. | + | Previous studies have reported that the interactions between tRNA primer and mRNA template as well as the reverse transcriptase are crucial in directing subsequent cDNA synthesis <a href="Ref1">(James E. Dahlberg et al.)</a>. Specifically, according to the model for reverse transcription proposed by <a href="Ref2">Kulpa et al.</a>, reverse transcription includes 5 steps (<a href="Fig1">Fig. 1</a>), in which the annealing of tRNA primer to the primer binding site (PBS) region on mRNA template is crucial for the synthesis of minus strand strong stop DNA (–ssDNA) and the following cDNA synthesis process. |
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Revision as of 07:46, 20 October 2019