Difference between revisions of "Team:IISER Kolkata/Experiments"

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<section class="sec white">
 
<section class="sec white">
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<h3>Bacterial protocol</h3>
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<h3>Bacterial Protocol</h3>
 
<div class="exp-list">
 
<div class="exp-list">
 
<a href="https://static.igem.org/mediawiki/2019/b/b9/T--IISER_Kolkata--1_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/b/b9/T--IISER_Kolkata--1_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>1</h1></div>
 
<div class="index"><h1>1</h1></div>
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<h5>LB media preparation</h5>
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<h5>LB Media Preparation</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/5/50/T--IISER_Kolkata--2_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/5/50/T--IISER_Kolkata--2_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>2</h1></div>
 
<div class="index"><h1>2</h1></div>
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<h5>SOB media preparation</h5>
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<h5>SOB Media Preparation</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/8/8d/T--IISER_Kolkata--3_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/8/8d/T--IISER_Kolkata--3_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>3</h1></div>
 
<div class="index"><h1>3</h1></div>
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<h5>50X TAE buffer</h5>
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<h5>50X TAE Buffer</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/b/bd/T--IISER_Kolkata--4_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/b/bd/T--IISER_Kolkata--4_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>4</h1></div>
 
<div class="index"><h1>4</h1></div>
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<h5>CCMB buffer preparation</h5>
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<h5>CCMB Buffer Preparation</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/f/fd/T--IISER_Kolkata--5_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/f/fd/T--IISER_Kolkata--5_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>5</h1></div>
 
<div class="index"><h1>5</h1></div>
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<h5>Calcium chloride competent cell preparation</h5>
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<h5>Calcium chloride Competent Cell Preparation</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/4/45/T--IISER_Kolkata--6_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/4/45/T--IISER_Kolkata--6_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>6</h1></div>
 
<div class="index"><h1>6</h1></div>
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<h5>Hanahan competent cell preparation</h5>
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<h5>Hanahan Competent Cell Preparation</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/5/52/T--IISER_Kolkata--7_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/5/52/T--IISER_Kolkata--7_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>7</h1></div>
 
<div class="index"><h1>7</h1></div>
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<h5>Electrocompetent cell preparation</h5>
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<h5>Electrocompetent Cell Preparation</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/f/f5/T--IISER_Kolkata--8_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/f/f5/T--IISER_Kolkata--8_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>8</h1></div>
 
<div class="index"><h1>8</h1></div>
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<h5>Heat shock transformation of chemically competent cells</h5>
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<h5>Heat Shock Transformation of Chemically Competent Cells</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/a/ac/T--IISER_Kolkata--9_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/a/ac/T--IISER_Kolkata--9_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>9</h1></div>
 
<div class="index"><h1>9</h1></div>
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<h5>Transformation by electroporation</h5>
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<h5>Transformation by Electroporation</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/2/23/T--IISER_Kolkata--10_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/2/23/T--IISER_Kolkata--10_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>10</h1></div>
 
<div class="index"><h1>10</h1></div>
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<h5>PCR amplification Q5 HF MM</h5>
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<h5>PCR Amplification: Q5® H-F MM</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/2/28/T--IISER_Kolkata--11_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/2/28/T--IISER_Kolkata--11_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>11</h1></div>
 
<div class="index"><h1>11</h1></div>
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<h5>PCR amplification TAQ POL</h5>
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<h5>PCR Amplification: Taq Pol</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/1/16/T--IISER_Kolkata--12_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/1/16/T--IISER_Kolkata--12_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>12</h1></div>
 
<div class="index"><h1>12</h1></div>
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<h5>Restriction digestion</h5>
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<h5>Restriction Digestion</h5>
 
</div></a>
 
</div></a>
 
<a href="https://static.igem.org/mediawiki/2019/4/40/T--IISER_Kolkata--13_bac.pdf" target="_blank"><div class="protocol">
 
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<a href="https://static.igem.org/mediawiki/2019/a/aa/T--IISER_Kolkata--14_bac.pdf" target="_blank"><div class="protocol">
 
<a href="https://static.igem.org/mediawiki/2019/a/aa/T--IISER_Kolkata--14_bac.pdf" target="_blank"><div class="protocol">
 
<div class="index"><h1>14</h1></div>
 
<div class="index"><h1>14</h1></div>
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<h5>SDS page electrophoresis</h5>
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<h5>SDS-PAGE Electrophoresis</h5>
 
</div></a>
 
</div></a>
 
</div>
 
</div>

Revision as of 04:20, 20 October 2019

Experiments

Once a great man said - "The true method of knowledge is through experiment." And in the quest to gain knowledge we did a lot of experiments.

Most of our work dealt with genetically engineering our bacterial chassis i.e. E. coli. Additionally, to support our model and to gain some meaningful insights into the system, we did a variety of experiment involving Macrophage and Leishmania major.

Bacterial Protocol

1

LB Media Preparation

2

SOB Media Preparation

3

50X TAE Buffer

4

CCMB Buffer Preparation

5

Calcium chloride Competent Cell Preparation

6

Hanahan Competent Cell Preparation

7

Electrocompetent Cell Preparation

8

Heat Shock Transformation of Chemically Competent Cells

9

Transformation by Electroporation

10

PCR Amplification: Q5® H-F MM

11

PCR Amplification: Taq Pol

12

Restriction Digestion

13

Ligation

14

SDS-PAGE Electrophoresis