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− | + | <b><a name="Fig1"></a>Figure 1. Workflow of the model.</a></b><br /> | |
+ | <p>Three Grey boxes indicate three major reaction steps in R-Evolution. Arrows indicate the reaction that certain substance is involved. White arrows indicate the case in which substances that originally exist in E.coli act as inputs. Red arrows indicate the case in which intermediates, which are produced in the previous reaction, are generated or involved in next reaction process. The blue arrow indicates the final output that we would like to observe. Inducer – IPTG or aTc (anhydrotetracycline). RT – reverse transcriptase. Cre – Cre recombinase. cDNA – complementary DNA.</p> | ||
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− | <b>Figure 2. Induced expression of | + | <b><a name="Fig2">Figure 2. Induced expression of RT and Cre.</a></b><br /> |
+ | <p><b>a)</b> Schematic diagram of the model. <b>b)</b> Dynamics of target protein. Horizontal axis shows the length of time. Vertical axis demonstrates the amount of protein (RT and Cre) within the system. RT and Cre are expressed under the same Lac operon. <b>c)</b> Dynamics of free lac operon. Horizontal axis shows the length of time. Vertical axis demonstrates the amount of free lac operon, i.e. the lac operon unbound by tetR dimer, within the system. The vertical magenta line indicates the moment when 50μM is added to the system.</p> | ||
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− | <b>Figure 3. Reverse transcription.</b><br /> | + | <b><a name="Fig3">Figure 3. Reverse transcription.</a></b> <br /> |
+ | <p><b>a)</b> Schematic diagram of the model. <b>b)</b> Dynamics of cDNA. Horizontal axis shows the length of time. Vertical axis demonstrates the amount of cDNA within the system.</p> | ||
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− | <b>Figure 4. Cre recombination ( | + | <b><a name="Fig4">Figure 4. Cre recombination (deterministic).</a></b><br /> |
+ | <p><b>a)</b> Schematic diagram of the model. <b>b-c)</b> Recombination when Cre is expressed under Lac operon. Dynamics of the percentage of un-recombined/ recombined Ptarget among all Ptargets is shown in <b>b</b>. Horizontal axis shows the length of time (8 hours, corresponding to R-Evolution’s function period). The distribution of the percentage of substances at the steady-state is shown in <b>c</b>. <b>d)</b> Yield of recombined Ptarget at different initial number of cDNA and Cre. The yield of recombined Ptarget is calculated as the percentage of recombined Ptarget among all Ptargets. The horizontal white line corresponds to current situation where the initial number of cDNA is 5 molecules in one E.coli. <b>e)</b> Yield of recombined Ptarget at different initial number of Cre when initial number of cDNA is 5 molecules. <b>f-g)</b> Recombination when Cre is expressed under different operon. Dynamics of the percentage of un-recombined/ recombined Ptarget among all Ptargets is shown in <b>f</b>. The distribution of the percentage of substances at the steady-state is shown in <b>g</b>.</p> | ||
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− | <b><a name="Fig5">Figure 5. Cre recombination (stochastic).</a></b><br />Horizontal axis shows the length of time (20min, corresponding to the duration of 1 E.coli replication cycle). Vertical axis demonstrates the number of recombined Ptarget. The initial number of Cre is 2 molecules in <b>a</b>, 3228 molecules in <b>b</b>. | + | <b><a name="Fig5">Figure 5. Cre recombination (stochastic).</a></b><br /> |
+ | <p>Horizontal axis shows the length of time (20min, corresponding to the duration of 1 E.coli replication cycle). Vertical axis demonstrates the number of recombined Ptarget. The initial number of Cre is 2 molecules in <b>a</b>, 3228 molecules in <b>b</b>.</p> | ||
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Revision as of 19:39, 19 October 2019