Difference between revisions of "Team:Fudan-TSI/Parts"

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{{Fudan-TSI}}<script src="https://code.jquery.com/jquery-1.11.3.min.js"></script>
+
{{Fudan-TSI}}
<html lang="en">
+
<html>
<!--
+
This html document is created by Tian Huang for Team Fudan iGEM 2018.
+
We make it compatible on laptop and mobile devices by using Materialize 1.0.0-rc.2.
+
-->
+
<!-- LC check on 2018-10-18 -->
+
<head>
+
    <meta charset="UTF-8">
+
  
    <!-- CSS -->
 
    <link rel="stylesheet" type="text/css" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-css.css&action=raw&ctype=text/css" />
 
  
    <!-- Font-awesome icons 4.7.0 -->
+
<script>
    <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" />
+
////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
  
    <!-- Materialize 1.0.0-rc.2 (Material Design like) -->
+
$(document).ready(function() {
    <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&action=raw&ctype=text/css">
+
  
    <!-- Clear default CSS settings; CSS reset -->
+
//remove the HQ_page id
    <style>
+
$("#HQ_page").attr('id','');
        *{margin: 0;padding: 0;list-style: none;}
+
        /* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */
+
  
        /** 清除内外边距 **/
+
//highlight current page on the menu
        body, h1, h3, h3, h4, h5, h6, hr, p, blockquote, /* structural elements 结构元素 */
+
highlight_current_page_menu();
        dl, dt, dd, ul, ol, li, /* list elements 列表元素 */
+
        pre, /* text formatting elements 文本格式元素 */
+
//accessing submenus
        form, fieldset, legend, button, input, textarea, /* form elements 表单元素 */
+
$(".menu_item").click(function(){
        th, td /* table elements 表格元素 */ {
+
$(".submenu_control_icon", this).toggleClass("open");
            margin: 0;
+
$(this).next(".submenu").fadeToggle(400);
            padding: 0;
+
});
        }
+
 +
//mobile menu access
 +
$(".igem_2019_team_mobile_bar").click(function(){
 +
$(this).next().toggleClass("displaying_menu");
 +
});
 +
 +
});
  
        /** 设置默认字体 **/
 
  
        /* @@@@ h1, h3, h3, h4, h5, h6 { font-size: 100%; }*/
+
function highlight_current_page_menu() {
        address, cite, dfn, em, var { font-style: normal; } /* 将斜体扶正 */
+
        code, kbd, pre, samp { font-family: courier new, courier, monospace; } /* 统一等宽字体 */
+
var page_url="https://2019.igem.org/";
        /* @@@@ small { font-size: 12px; } /* 小于 12px 的中文很难阅读,让 small 正常化 */
+
page_url = page_url + wgPageName;
 +
$("a[href$='"+ page_url +"']").children().addClass("current_page");
 +
 +
//if the page is in a submenu, open the submenu and make the appropiate changes
 +
if( $( ".current_page" ).hasClass( "submenu_item" )){
 +
 +
$(".current_page").parent().parent().fadeToggle(400);
 +
$(".current_page").parent().parent().prev().addClass("current_page");
 +
$(".menu_item.current_page > .submenu_control_icon").toggleClass("open");
 +
 +
}
 +
}
  
        /** 重置列表元素 **/
 
        ul, ol { list-style: none; }
 
  
        /** 重置文本格式元素 **/
+
////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////////
        a { text-decoration: none; }
+
</script>
        a:hover { text-decoration: underline; }
+
  
  
        /** 重置表单元素 **/
+
<style>
        legend { color: #000; } /* for ie6 */
+
/**************************************************************************************************************************************************************************************************/
        fieldset, img { border: 0; } /* img 搭车:让链接里的 img 无边框 */
+
        button, input, select, textarea { font-size: 100%; } /* 使得表单元素在 ie 下能继承字体大小 */
+
        /* 注:optgroup 无法扶正 */
+
  
        /** 重置表格元素 **/
 
        table { border-collapse: collapse; border-spacing: 0; }
 
  
    </style>
+
 
    <title>2019 Team:Fudan-TSI Parts</title>
+
/**************************************************************************************************************************************************************************************************/
 +
/* DEFAULT WIKI SETTINGS */
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
 
 +
#home_logo, #sideMenu { display:none; }
 +
#sideMenu, #top_title, .patrollink  {display:none;}
 +
#content { margin-left:0px; margin-top:-7px; padding:0px; width:100%;}
 +
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 +
#bodyContent a[href ^="https://"], .link-https { padding-right:0px;}
 +
 +
 +
 
 +
 
 +
/**************************************************************************************************************************************************************************************************/
 +
/* MENU */
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/*wrapping for the menu*/
 +
.igem_2019_team_menu {
 +
background-color:#cecece;
 +
border-left: 1px solid #635d5d;
 +
float:right;
 +
height:100vh;
 +
max-width: 270px;
 +
overflow-y: auto;
 +
overflow-x: hidden;
 +
padding:0px;
 +
position:fixed;
 +
right:0%;
 +
text-align:left;
 +
width: 15%;
 +
}
 +
 
 +
/*controlling menu visibility*/
 +
.igem_2019_team_menu.displaying_menu{
 +
display:block;
 +
}
 +
 
 +
/*links in the menu*/
 +
.igem_2019_team_menu  a {
 +
color: #484848;
 +
text-decoration:none;
 +
}
 +
 
 +
/*images in the menu*/
 +
.igem_2019_team_menu img {
 +
width:100%;
 +
}
 +
 
 +
/*level 1 menu items*/
 +
.igem_2019_team_menu .menu_item {
 +
    background-color: #cecece;
 +
    border-bottom: 1px solid #635d5d;
 +
    clear: both;
 +
color: #484848;
 +
    cursor: pointer;
 +
float: left;
 +
    font-size: 120%;
 +
    font-weight: bold;
 +
    padding: 15px 0px 15px 5%;
 +
  width: 100%;
 +
}
 +
 
 +
/*level 1 menu items without submenus*/
 +
.igem_2019_team_menu .menu_item.direct_link {
 +
color: #484848;
 +
padding-left: 15%;
 +
}
 +
 
 +
 +
/*level 1 menu items on hover*/
 +
.igem_2019_team_menu .menu_item:hover {
 +
background-color: #ecb656 !important;
 +
}
 +
 
 +
/*icon for expanding and collapsing level 1 menut items*/
 +
.igem_2019_team_menu .menu_item .submenu_control_icon {
 +
color: #484848;
 +
float: left;
 +
width: 10%;
 +
}
 +
 
 +
/* submenu icon  "-"*/
 +
.igem_2019_team_menu .menu_item .submenu_control_icon::before {
 +
content: "+";
 +
}
 +
 
 +
/* submenu icon  "-"*/
 +
.igem_2019_team_menu .menu_item .submenu_control_icon.open::before {
 +
content: "-";
 +
}
 +
 
 +
 +
/*level 2 menu (submenu) wrapper*/
 +
.igem_2019_team_menu .submenu{
 +
background-color: #ececec;
 +
clear:both;
 +
display:none;
 +
float: left; 
 +
width:100%;
 +
}
 +
 
 +
/*level 2 menu (submenu) item*/
 +
.igem_2019_team_menu .submenu .submenu_item {
 +
border-bottom: 1px solid #cecece;
 +
  color: #635d5d;
 +
    height: 30px;
 +
  float: left;
 +
    font-size: 110%;
 +
font-weight: bold;
 +
    padding: 12px 0px 0px 15%;
 +
    width: 100%;
 +
}
 +
 
 +
/*level 2 menu (submenu) items on hover*/
 +
.igem_2019_team_menu .submenu .submenu_item:hover {
 +
background-color: #ecb656 !important;
 +
}
 +
 
 +
/*color for highlighting current page on the wiki*/
 +
.igem_2019_team_menu .submenu .submenu_item.current_page,
 +
.igem_2019_team_menu .menu_item.current_page,
 +
.igem_2019_team_menu .menu_item.direct_link.current_page {
 +
background-color:#a2d3d0;
 +
}
 +
 
 +
 
 +
/*mobile menu bar styling*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/*mobile bar that controls the menu*/
 +
.igem_2019_team_mobile_bar {
 +
background-color:#ececec;
 +
border-bottom: 1px solid #cecece;
 +
cursor:pointer;
 +
display:none;
 +
float:left;
 +
margin-top: 0;
 +
padding: 5px 0;
 +
position:fixed;
 +
width:100%;
 +
}
 +
 +
/*mobile logo*/
 +
.igem_logo_mobile {
 +
float:left;
 +
padding-left: 5%;
 +
width: 30%;
 +
}
 +
 
 +
/*image within the mobile logo*/
 +
.igem_logo_mobile img {
 +
width:70px;
 +
}
 +
 +
/*mobile expand collapse button*/
 +
.igem_menu_control_mobile {
 +
float:right;
 +
padding-right:5%;
 +
padding-top:5px;
 +
text-align:right;
 +
width: 30%;
 +
}
 +
 
 +
/*image for mobile expand collapse button*/
 +
.igem_menu_control_mobile img {
 +
width:25px;
 +
}
 +
 
 +
 
 +
/*add extra padding to the menu to improve mobile scrolling*/
 +
.menu_padding{
 +
float:left;
 +
height:100px;
 +
}
 +
 
 +
 
 +
/**************************************************************************************************************************************************************************************************/
 +
/* CONTENT OF THE PAGE */
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/* general wrapper for the content */
 +
.igem_2019_team_content {
 +
background-color:white;
 +
display:block;
 +
width: 87%;
 +
}
 +
 
 +
/* subwrapper to center content */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper {
 +
margin:auto;
 +
max-width: 1400px;
 +
width:90%;
 +
}
 +
 
 +
 
 +
 
 +
/*general styling*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/*size for title h tags*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h1 { font-size: 210%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h2 { font-size: 190%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h3 { font-size: 170%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h4 { font-size: 150%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h5 { font-size: 140%;}
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h6 { font-size: 130%;}
 +
 
 +
 
 +
/*titles h1, h2*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h1, .igem_2019_team_content .igem_2019_team_column_wrapper h2 {
 +
border-bottom:0px;
 +
color: white;
 +
font-family: "Arial Black", Gadget, sans-serif;
 +
padding: 10px 0px;
 +
}
 +
 
 +
/*titles h3, h3, h5, h6 */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h3,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h4,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h5,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper h6 {
 +
border-bottom:0px;
 +
color: white; 
 +
font-family: "Arial Black", Gadget, sans-serif;
 +
padding: 5px 0px;
 +
}
 +
 
 +
/* text p tag*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper p {
 +
font-size: 130%;
 +
font-family: Arial, Helvetica, sans-serif;
 +
padding: 5px 0px;
 +
text-align: left;
 +
color: white;
 +
}
 +
 
 +
/* Links a tag*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper a {
 +
color: #00a19b;
 +
font-weight: bold;
 +
text-decoration: underline;
 +
text-decoration-color:#00a19b;
 +
transition: all 0.4s ease;
 +
-webkit-transition: all 0.4s ease;
 +
-moz-transition: all 0.4s ease;
 +
-ms-transition: all 0.4s ease;
 +
-o-transition: all 0.4s ease;
 +
}
 +
 
 +
/* hover for the links */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper a:hover {
 +
color: #085156;
 +
text-decoration:none;
 +
}
 +
 
 +
 
 +
/* Table tag*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper table {
 +
border: 1px solid #635d5d;
 +
border-collapse: collapse;
 +
font-size: 130%;
 +
width: 100%;
 +
}
 +
 
 +
/* table cells */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper td {
 +
border: 1px solid #cecece;
 +
border-collapse: collapse;
 +
font-size: 105%;
 +
padding: 10px;
 +
vertical-align: text-top;
 +
}
 +
 
 +
/* table headers */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper th {
 +
background-color:#cecece;
 +
border: 1px solid #635d5d;
 +
border-collapse: collapse;
 +
font-size: 110%;
 +
padding: 10px;
 +
vertical-align: text-top;
 +
}
 +
 
 +
 
 +
 
 +
/* non numbered lists */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ul, .igem_2019_team_content .igem_2019_team_column_wrapper ol {
 +
font-size: 130%;
 +
font-family: Arial, Helvetica, sans-serif;
 +
padding:0px 20px;
 +
}
 +
 
 +
 
 +
/*font sizing within list nesting*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ul li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ul ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ul ol li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ul li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ul ol ol li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ul ul li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ol ul li,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper ol ol ol li, .igem_2019_team_content .igem_2019_team_column_wrapper ol ul ol li{ font-size: 76%; }
 +
 
 +
 
 +
 
 +
/*layout classes*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
/*main layout class */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column  {
 +
float:left;
 +
margin: 1% 2%;
 +
padding: 0px;
 +
}
 +
 
 +
/* 100% */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size { width:96%; }
 +
 
 +
/* 66% */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size { width: 62.6%; }
 +
 
 +
/* 33% */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size { width: 29.3%; }
 +
 
 +
 
 +
 
 +
 
 +
/*all images*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size img,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size img,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size img {
 +
margin-bottom: 15px;
 +
width: 100%;
 +
}
 +
 
 +
 
 +
/* page break */
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .clear {
 +
clear:both;
 +
}
 +
/*add extra space to page break with clear class*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .clear.extra_space {
 +
height: 30px;
 +
}
 +
 
 +
/* horizontal line to divide the page*/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .line_divider {
 +
    border-top: 1px solid #cecece;
 +
  margin: auto;
 +
  width: 98%;
 +
}
 +
 
 +
 
 +
 
 +
 +
/*support classes*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
 
 +
/*Button  */
 +
/************************************************/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link {
 +
font-size: 130%;
 +
margin: 30px auto;
 +
text-align: center;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link a  {
 +
background-color: #00a19bad !important;
 +
color: #000 !important;
 +
font-weight: bold;
 +
margin: auto;
 +
text-decoration: none !important;
 +
padding: 10px 15px !important;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .button_link a:hover {
 +
background-color: #ffb819 !important; 
 +
}
 +
 
 +
 +
 
 +
/*highlight */
 +
/************************************************/
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight {
 +
padding: 15px 20px;
 +
}
 +
 
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight p,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h1,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h2,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h3,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h4,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h5,
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight h6 {
 +
padding: 5px 15px;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_background {
 +
background-color: #ececec;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_top {
 +
    border-top: 4px solid #00a19bad;
 +
}
 +
 
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_A_full {
 +
    border: 4px solid #00a19bad;
 +
}
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_top {
 +
    border-top: 4px solid #ffb819
 +
}
 +
 
 +
 
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .highlight.decoration_B_full {
 +
    border: 4px solid #ffb819;
 +
}
 +
 
 +
 
 +
 
 +
 
 +
/*mobile*/
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
 
 +
/* 1800px  */
 +
/************************************************/
 +
@media only screen and (max-width: 1800px) {
 +
.igem_2019_team_content { width: 85%;}
 +
.igem_2019_team_menu {display:block;}
 +
 +
}
 +
 
 +
/* 1400px  */
 +
/************************************************/
 +
@media only screen and (max-width: 1400px) {
 +
.igem_2019_team_menu .menu_item { font-size:100%;}
 +
.igem_2019_team_menu .submenu .submenu_item { font-size:90%;}
 +
.igem_2019_team_menu {display:block;}
 +
}
 +
 
 +
 
 +
/* 1100px  */
 +
/************************************************/
 +
@media only screen and (max-width: 1100px) {
 +
.igem_2019_team_content {width:100%; margin-left:0px;}
 +
 +
.igem_2019_team_menu {display:none;float:right;margin-top:47px;max-width:100%;position:fixed;width:25%;}
 +
 +
.igem_2019_team_mobile_bar {display:block;}
 +
 +
.igem_2019_team_content .igem_2019_team_column_wrapper .column.full_size, .igem_2019_team_content .igem_2019_team_column_wrapper .column.two_thirds_size,.igem_2019_team_content .igem_2019_team_column_wrapper .column.third_size {width:96%; }
 +
 
 +
}
 +
 
 +
/* 850px  */
 +
/************************************************/
 +
@media only screen and (max-width: 850px) {
 +
.igem_2019_team_menu {width:40%;}
 +
}
 +
 
 +
/*500px  */
 +
/************************************************/
 +
@media only screen and (max-width: 500px) {
 +
.igem_2019_team_menu {min-width:100%;width:100%;}
 +
}
 +
 
 +
 
 +
/**************************************************************************************************************************************************************************************************/
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</style>
 +
 
 +
 
 +
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
 +
<!--- THIS IS WHERE THE HTML BEGINS --->
 +
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
 +
 
 +
<head>
 +
 
 +
<!-- This tells the browser that your page is responsive -->
 +
<meta name="viewport" content="width=device-width, initial-scale=1">
 +
 +
<script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/jQuery&amp;action=raw&amp;ctype=text/javascript"></script>
 +
 +
 +
 
</head>
 
</head>
  
<body>
+
<link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" />
<!-- Fudan div at igem.org -->
+
 
<div id="FudanWrapper" class="white">
+
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
    <div id="FudanBody" class="white">
+
<!--- Menu --->
        <header>
+
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
            <!-- empty bar -->
+
            <div id="emptyBar" style="position:relative;width: 100%;"></div>
+
 
 +
<style>
 +
 +
    *{margin: 0;padding: 0;list-style: none;}
 +
/* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */
 +
 
 +
/** 清除内外边距 **/
 +
body, h1, h3, h3, h4, h5, h6, hr, p, blockquote, /* structural elements 结构元素 */
 +
dl, dt, dd, ul, ol, li, /* list elements 列表元素 */
 +
pre, /* text formatting elements 文本格式元素 */
 +
form, fieldset, legend, button, input, textarea, /* form elements 表单元素 */
 +
th, td /* table elements 表格元素 */ {
 +
margin: 0;
 +
padding: 0;
 +
}
 +
 
 +
/** 设置默认字体 **/
 +
 
 +
/* @@@@ h1, h3, h3, h4, h5, h6 { font-size: 100%; }*/
 +
address, cite, dfn, em, var { font-style: normal; } /* 将斜体扶正 */
 +
code, kbd, pre, samp { font-family: courier new, courier, monospace; } /* 统一等宽字体 */
 +
/* @@@@ small { font-size: 12px; } /* 小于 12px 的中文很难阅读,让 small 正常化 */
 +
 
 +
/** 重置列表元素 **/
 +
ul, ol { list-style: none; }
 +
 
 +
/** 重置文本格式元素 **/
 +
a { text-decoration: none; }
 +
a:hover { text-decoration: underline; }
 +
 
 +
 
 +
/** 重置表单元素 **/
 +
legend { color: #000; } /* for ie6 */
 +
fieldset, img { border: 0; } /* img 搭车:让链接里的 img 无边框 */
 +
button, input, select, textarea { font-size: 100%; } /* 使得表单元素在 ie 下能继承字体大小 */
 +
/* 注:optgroup 无法扶正 */
 +
 
 +
/** 重置表格元素 **/
 +
table { border-collapse: collapse; border-spacing: 0; }
 +
 +
</style>
 +
 
 +
 
 +
 
 +
 
 +
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------    Content begins    --------------------------------------------------------------------------------------------------------------------------------->
 +
<!---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------->
 +
 
 +
 +
 +
 +
 +
 +
<link rel="stylesheet" href="https://cdn.staticfile.org/twitter-bootstrap/3.3.7/css/bootstrap.min.css">
 +
<link rel="stylesheet" href="https://stackpath.bootstrapcdn.com/bootstrap/4.3.1/css/bootstrap.min.css" integrity="sha384-ggOyR0iXCbMQv3Xipma34MD+dH/1fQ784/j6cY/iJTQUOhcWr7x9JvoRxT2MZw1T" crossorigin="anonymous">
 +
 +
 +
<link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/materialize.css&amp;action=raw&amp;ctype=text/css">
 +
 +
<style>
 +
 +
body{
 +
margin:0;
 +
padding:0;
 +
background-color:#08273a;
 +
}
 +
a{
 +
text-decoration:none;
 +
}
 +
#global_wrapper{
 +
width:100%;
 +
height:auto;
 +
margin:0;
 +
position:absolute;
 +
}
 +
#navUl{
 +
width:100%;
 +
height:110px;
 +
padding:40px 0 0 0;
 +
overflow:visible;
 +
position:fixed;
 +
list-style:none;
 +
z-index:999;
 +
background-color:#08273a;
 +
margin:0;
 +
top:0;
 +
}
 +
#mobileNav{
 +
width:100%;
 +
height:80px;
 +
padding:20px 0 0 0;
 +
top:0;
 +
background-color:#001d2a;
 +
position:fixed;
 +
display:none;
 +
text-align:center;
 +
z-index:999;
 +
}
 +
#mobileNav img{
 +
display:none;
 +
margin:0;
 +
padding:0;
 +
}
 +
#mobileLogo{
 +
display:inline-block;
 +
}
 +
#mobileControl{
 +
float:right;
 +
display:inline-block;
 +
margin-right:15px;
 +
margin-top:3px;
 +
}
 +
#mobileCtrl{
 +
height:25px;
 +
}
 +
#mobileTeamName{
 +
display:inline-block;
 +
}
 +
#navImg{
 +
display:inline-block;
 +
float:left;
 +
height:70px;
 +
width:auto;
 +
position:relative;
 +
margin-left:4%;
 +
margin-top:0;
 +
}
 +
.logo{
 +
height:55px;
 +
width:auto;
 +
margin-top:1.3%;
 +
}
 +
.teamname{
 +
height:28px;
 +
}
 +
 
 +
#navBar{
 +
float:right;
 +
position:relative;
 +
width:auto;
 +
display:inline-block;
 +
margin-right:4%;
 +
}
 +
.navLi{
 +
float:left;
 +
display:inline-block;
 +
margin-top:3%;
 +
color:white;
 +
font-size:20px;
 +
position:relative;
 +
margin-left:18px;
 +
text-align:center;
 +
font-family:"Lucida Grande", "Lucida Sans Unicode", "Lucida Sans", "DejaVu Sans", Verdana, "sans-serif";
 +
text-decoration:none;
 +
}
 +
.navA{
 +
display:block;
 +
text-align:center;
 +
color:white;
 +
text-decoration:none;
 +
}
 +
.navA2{
 +
display:block;
 +
overflow:visible;
 +
color:white;
 +
height:auto;
 +
}
 +
.ul2{
 +
list-style:none;
 +
position:absolute;
 +
display:none;
 +
overflow:hidden;
 +
padding:10px 0 0 0 !important;
 +
margin:0 !important;
 +
font-size:17px;
 +
left:50%;
 +
transform:translateX(-50%);
 +
border-bottom-left-radius: 10px;
 +
border-bottom-right-radius: 10px;
 +
background:linear-gradient(#08273a,rgba(0,138,201,1));
 +
}
 +
.li2{
 +
padding:0;
 +
margin:10px 20px;
 +
text-align:center;
 +
display:block;
 +
}
 +
.navA:link,.navA2:link{
 +
text-decoration:none;
 +
}
 +
.navA:visited{
 +
color:white;
 +
}
 +
.navA2:visited,.navA2:active{
 +
color:white;
 +
}
 +
.navA:hover{
 +
color:#7dded4;
 +
}
 +
.navA2:hover{
 +
color:#7dded4;
 +
}
 +
.jqhover{
 +
color:#7dded4;
 +
}
 +
.navA:hover{
 +
text-decoration:none;
 +
}
 +
.navA:active{
 +
text-decoration:none;
 +
color:white;
 +
}
 +
 
 +
</style>
 +
 
 +
 +
 
 +
 +
<style>
 +
 +
#pageContent{
 +
margin:100px 0 0 0;
 +
text-align:center;
 +
}
 +
 +
.row{
 +
clear:both!important;
 +
}
 +
 
 +
.title1{
 +
font-size:2.3rem;
 +
text-align:center;
 +
color:white;
 +
display:block;
 +
margin-top:10%;
 +
margin-bottom:7%;
 +
line-height:110%;
 +
}
 +
.title2{
 +
color:white;
 +
text-align:left;
 +
font-size:2rem;
 +
line-height:130%;
 +
display:block;
 +
width:100%;
 +
}
 +
.title3{
 +
font-size:1.4rem;
 +
color:white;
 +
text-align:left !important;
 +
display:block;
 +
width:100%;
 +
line-height:110%;
 +
padding-left:2%;
 +
}
 +
.para1{
 +
color:white;
 +
text-align:justify !important;
 +
align-items:flex-start;
 +
line-height:140%;
 +
font-size:1.3rem;
 +
margin-bottom:50px !important;
 +
width:100%;
 +
margin:auto 0;
 +
}
 +
.para1 a{
 +
text-decoration:underline !important;
 +
color:white;
 +
}
 +
.para1 a:visited, .para1 a:active{
 +
color:white;
 +
}
 +
.para1 a:hover{
 +
color:rgba(96,255,249,1.00)!important;
 +
}
 +
.content1{
 +
margin:7% auto;
 +
}
 +
.pic2{
 +
width:150%;
 +
}
 +
.reverseDiv{
 +
position:relative !important;
 +
float:right !important;
 +
}
 +
 
 +
 +
.paraUl{
 +
list-style-type:decimal !important;
 +
list-style-position:outside;
 +
padding-left:auto;
 +
font-size:1rem;
 +
}
 +
.paraUl li{
 +
padding-right:4% ;
 +
}
 +
 +
 +
#containerWithLeftNav{
 +
display:block;
 +
margin-left:25%;
 +
}
 +
.legend{
 +
color:white;
 +
text-align:center;
 +
}
 +
.legend>div{
 +
width:100%;
 +
text-align:justify!important;
 +
font-size:1.1rem;
 +
}
 +
.legends{
 +
margin:auto 15%;
 +
}
 +
 
 +
 
 +
</style>
 +
 +
<style>
 +
 +
@media only screen and (max-width:1200px){
 +
#mobileBar{
 +
margin-left:4%;
 +
}
 +
.navLi{
 +
font-size:18px;
 +
margin-top:3.5%;
 +
}
 +
}
 +
 +
 +
@media only screen and (max-width:1100px){
 +
 +
#navUl{
 +
display:block;
 +
float:right;
 +
margin-top:0;
 +
top:80px;
 +
right:0;
 +
background-color:rgba(0,0,0,0);
 +
padding:0;
 +
}
 +
 +
#mobileNav{
 +
display:block;
 +
}
 +
 +
#navImg{
 +
display:none;
 +
}
 +
 +
 +
#navBar{
 +
margin:0 1% 0 0;
 +
padding-right:2%;
 +
padding-left:1%;
 +
position:relative;
 +
display:none;
 +
}
 +
 +
#mobileNav img{
 +
display:inline-block;
 +
margin:5px 0;
 +
padding:0;
 +
}
 +
#mobileControl{
 +
margin-top:8px;
 +
}
 +
 
 +
.logo{
 +
height:45px;
 +
margin-top:7px;
 +
}
 +
.navLi{
 +
display:block;
 +
position:relative;
 +
float:right;
 +
clear:both;
 +
white-space:nowrap;
 +
text-align:right;
 +
margin:0;
 +
height:60px;
 +
width:100%;
 +
background-color:#00324a;
 +
}
 +
.navA{
 +
display:block;
 +
text-align:right;
 +
position:relative;
 +
float:right;
 +
padding:20px 20px !important;
 +
}
 +
.n2{
 +
display:none;
 +
width:0;
 +
position:relative;
 +
float:left;
 +
text-align:right;
 +
}
 +
.ul2{
 +
background:none;
 +
padding-top:0;
 +
background-color:#00557b;
 +
text-align:right;
 +
display:block;
 +
position:relative;
 +
float:right;
 +
right:100%;
 +
transform:translateX(0%);
 +
top:0;
 +
transform:translateY(1%);
 +
border-radius:0 0 0 10px;
 +
}
 +
.open{
 +
display:block;
 +
}
 +
 +
.title2{
 +
font-size:1.8rem;
 +
line-height:140%;
 +
}
 +
.para1{
 +
font-size:1.3rem;
 +
}
 +
.pic2{
 +
width:120%;
 +
}
 +
 +
.highlightIcon{
 +
margin:20% auto 10% auto;
 +
}
 +
.highlightTitle{
 +
margin-bottom:15%;
 +
}
 +
 
 +
 +
}
 +
 +
 +
@media only screen and (max-width:992px){
 +
 +
#navUl{
 +
display:block;
 +
float:right;
 +
margin:0;
 +
top:80px;
 +
right:0;
 +
}
 +
 +
#mobileNav{
 +
display:block;
 +
}
 +
 +
#navImg{
 +
display:none;
 +
}
 +
 +
#navTeamName{
 +
display:none;
 +
}
 +
 +
#navBar{
 +
margin:0 1% 0 0 ;
 +
margin-right:2%;
 +
padding-right:1%;
 +
padding-left:1%;
 +
position:relative;
 +
display:none;
 +
}
 +
#mobileControl{
 +
margin-top:10px;
 +
}
 +
#mobileNav img{
 +
display:inline-block;
 +
margin:5px 0;
 +
padding:0;
 +
}
 +
.logo{
 +
height:40px;
 +
}
 +
.navLi{
 +
display:block;
 +
position:relative;
 +
float:right;
 +
clear:both;
 +
white-space:nowrap;
 +
text-align:right;
 +
height:60px;
 +
width:100%;
 +
}
 +
.navA{
 +
display:block;
 +
text-align:right;
 +
position:relative;
 +
float:right;
 +
font-size:15px;
 +
}
 +
.n2{
 +
display:none;
 +
width:0;
 +
position:relative;
 +
float:left;
 +
}
 +
.ul2{
 +
font-size:13px;
 +
}
 +
.open{
 +
display:block;
 +
}
 +
 +
 
 +
 +
#animation_container{
 +
display:none;
 +
}
 +
#teamLogo{
 +
display:block;
 +
}
 +
 
 +
 +
.title2{
 +
font-size:1.5rem;
 +
}
 +
.para1{
 +
font-size:1.2rem;
 +
}
 +
.pic2{
 +
width:100%;
 +
}
 +
 
 +
 +
}
 +
 +
 +
@media only screen and (max-width:768px){
 +
.col-md-4{
 +
clear:both;
 +
}
 +
.col-md-8{
 +
clear:both;
 +
}
 +
.pic2{
 +
margin-bottom:10%;
 +
width:80%;
 +
}
 +
.title2{
 +
font-size:1.2rem;
 +
}
 +
.para1{
 +
font-size:1rem;
 +
}
 +
.pic2{
 +
width:80%;
 +
}
 +
#sponser img {
 +
width:60%;
 +
}
 +
 +
#containerWithLeftNav{
 +
margin-left:auto;
 +
}
 +
 +
}
 +
 +
</style>
 +
 
 +
 
 +
 +
 +
 
 +
 
 +
<style>
 +
#footContainer{
 +
width:90%;
 +
}
 +
#FudanFooter{
 +
margin:auto 0;
 +
width:100%;
 +
padding:3% 0;
 +
}
 +
#FudanFooter #usefulLinks {
 +
color: #cacaca;
 +
padding-left: 1rem;
 +
}
 +
 
 +
#FudanFooter #usefulLinks ul {
 +
font-size: 13px;
 +
line-height: 14px;
 +
border-top: solid 2px;
 +
color: inherit;
 +
text-decoration: none;
 +
padding-top: 5px;
 +
margin:0;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div {
 +
color: #cacaca;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div:hover {
 +
color: white;
 +
}
 +
 
 +
#FudanFooter #usefulLinks a {
 +
color: inherit;
 +
}
 +
 
 +
#FudanFooter #usefulLinks a:hover {
 +
text-decoration: underline;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div.active,
 +
#FudanFooter #usefulLinks div.active a {
 +
color: white;
 +
}
 +
 
 +
#FudanFooter #usefulLinks div.active ul {
 +
border-top: solid white 2px;
 +
}
 +
 
 +
#FudanFooter #usefulLinks li {
 +
padding: 3px 0 6px 3px;
 +
list-style:none;
 +
}
 +
 
 +
#usefulLinks span {
 +
font-size: 20px;
 +
}
 +
 +
 
 +
#FudanFooter div.footer-copyright {
 +
font-size: 13px;
 +
line-height: 15px;
 +
}
 +
.footerUl{
 +
margin:2% 4%;
 +
}
 +
</style>
 +
 
 +
<script>
 +
 +
$(document).ready(function(){
 +
 +
 +
var winWidth=$(window).width();
 +
 +
if (winWidth>1100){
 +
$(".navA").mouseenter(function(){
 +
$(this).parent().find(".ul2").stop().fadeIn(400);
 +
});
 +
$(".navLi").mouseleave(function(){
 +
$(this).find(".ul2").stop().fadeOut(400);
 +
});
 +
 +
 +
}
 +
else{
 +
$(".navA:not(.noSubmenu)").removeAttr("href");
 +
$("#mobileNav").click(function(){
 +
$("#navBar").toggle();
 +
});
 +
$(document).click(function(event){
 +
var m = $("#mobileNav,#navBar");
 +
if (!m.is(event.target)){
 +
if (m.has(event.target).length===0){
 +
$("#navBar").hide();
 +
$(".open").parent().find(".navA").css("color","white");
 +
$(".open").removeClass("open");
 +
}
 +
 
 +
}
 +
});
 +
$(".navLi").click(function(){
 +
if ($(this).find(".n2").hasClass("open")){
 +
$(".open").parent().find(".navA").css("color","white");
 +
$(this).find(".n2").removeClass("open");
 +
}
 +
else{
 +
$(".open").parent().find(".navA").css("color","white");
 +
$(".open").removeClass("open");
 +
$(this).find(".n2").addClass("open");
 +
$(this).find(".navA").css("color","#7dded4");
 +
}
 +
});
 +
}
 +
 +
 +
 +
 +
 +
 +
});
 +
 +
 +
</script>
 +
 +
 +
 +
<div id="global_wrapper">
 +
<div id="mobileNav">
 +
 +
<div id="mobileLogo"><img src="https://static.igem.org/mediawiki/2019/d/d3/T--Fudan-TSI--HomepageLogo.gif" class="logo"></div>
 +
<div id="mobileControl"><img src="https://static.igem.org/mediawiki/2019/thumb/a/ae/T--Fudan-TSI--mobileCtrl.gif/683px-T--Fudan-TSI--mobileCtrl.gif" id="mobileCtrl"></div>
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</div>
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<ul id="navUl">
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</li>
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<ul id="navBar">
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<li class="navLi"><a class="navA noSubmenu" href="https://2019.igem.org/Team:Fudan-TSI">Home</a></li>
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<li class="navLi">
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<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Description">Project</a>
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<div class="n2">
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<ul class="ul2">
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Description">Description</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Design">Design</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Design" style="white-space:nowrap">Applied Design</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Experiment">Experiment</a></li>
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</ul>
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</div>
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</li>
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<li class="navLi">
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<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Results">Results</a>
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<div class="n2">
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<ul class="ul2">
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Reverse_Transcription">Reverse Transcription</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Results/Recombination">Recombination</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Measurement">Measurement</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Notebook">Notebook</a></li>
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</ul>
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</div>
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</li>
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<li class="navLi">
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<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Model_Software">Model</a>
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<div class="n2">
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<ul class="ul2">
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Model">Modeling</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Software">Software</a></li>
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</ul>
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</div>
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</li>
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<li class="navLi">
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<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Parts">Parts</a>
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<div class="n2">
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<ul class="ul2">
 +
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Basic_Part">Basic Parts</a></li>
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<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Composite_Part">Composite Parts</a></li>
 +
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Improve">Improved Parts</a></li>
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<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Part_Collection">Part Collection</a></li>
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</ul>
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</div>
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</li>
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<li class="navLi">
 +
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Human_Practices">Human Practices</a>
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<div class="n2">
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<ul class="ul2">
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<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Public_Engagement">Education &amp; <br />Public Engagement</a></li>
 +
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Integrated_Human_Practice">Integrated <br />Human Practice</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Collaborations">Collaboration</a></li>
 +
<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Safety">Safety</a></li>
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</ul>
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</div>
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</li>
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 +
<li class="navLi">
 +
<a class="navA" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team</a>
 +
<div class="n2">
 +
<ul class="ul2">
 +
<li class="li2"><a class="navA2" style="white-space:nowrap;" href="https://2019.igem.org/Team:Fudan-TSI/Team">Team Members</a></li>
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<li class="li2"><a class="navA2" href="https://2019.igem.org/Team:Fudan-TSI/Team/Attribution">Attribution</a></li>
 +
</ul>
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</div>
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</li>
 +
 +
<li class="navLi"><a class="navA noSubmenu" href="https://igem.org/2019_Judging_Form?id=3257">Judging</a></li>
 +
 +
</ul>
 +
 +
 +
</ul>
  
            <!-- Navigation bar 2019-9-15 -->
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
            <nav id="topNav" class="white z-depth-0_5">
+
<!---- Cover ---->
                <div class="nav-wrapper">
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
                    <div id="teamLogo" class="brand-logo">
+
                        <a href="/Team:Fudan-TSI" target="_self"><img alt="2019 team logo" src="https://static.igem.org/mediawiki/2019/3/3c/T--Fudan-TSI--Logo0.gif"></a>
+
<div id="pageCover">
                    </div>
+
                    <ul id="nav-mobile" class="right">
+
<script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/bkg&amp;action=raw&amp;ctype=text/javascript"></script>
                        <li>
+
<script>
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+
$(document).ready(function($){
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+
var $root = $('html, body');
                            </a>
+
$('a[href^="#"]').click(function() {
                        </li>
+
var href = $.attr(this, 'href');
                    </ul>
+
$root.animate({
                </div>
+
scrollTop: $(href).offset().top
            </nav>
+
}, 1000, function () {
            <!-- Dropdown and List elements in navigation bar -->
+
window.location.hash = href;
            <!-- Slide-out navigator contents 2019-9-15 -->
+
});
            <ul id="slide-out" class="sidenav">
+
return false;
                <li style="padding: 0"><div class="sidenavBanner">
+
});
                    <div class="background">
+
})
                    </div>
+
</script>
                    <p class="flow-text" style="width:100%;text-align:center"><span class="white-text">Parts</span></p>
+
                </div></li>
+
<svg id="demo" viewBox="0 0 1600 600" preserveAspectRatio="xMidYMid slice" style="z-index: -100;">
                <li>
+
  <defs>
                    <ul class="collapsible expandable">
+
<linearGradient id="grad1" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
                        <li class="onThisPageNav"><span>Team: Fudan-TSI</span></li>
+
  <stop id="stop1a" offset="0%" stop-color="#12a3b4"></stop>
<li><div class="collapsible-header"><span>Project</span></div>
+
  <stop id="stop1b" offset="100%" stop-color="#ff509e"></stop>
    <div class="collapsible-body"><ul>
+
</linearGradient>
        <li><a href="/Team:Fudan-TSI/Description">Background</a></li>
+
<linearGradient id="grad2" x1="0" y1="0" x2="1" y2="0" color-interpolation="sRGB">
        <li><a href="/Team:Fudan-TSI/Design">Design</a></li>
+
  <stop id="stop2a" offset="0%" stop-color="#e3bc13"></stop>
        <li><a href="/Team:Fudan-TSI/Applied_Design">Applied Design</a></li>
+
  <stop id="stop2b" offset="100%" stop-color="#00a78f"></stop>
        <li><a href="/Team:Fudan-TSI/Experiments">Experiments</a></li>
+
</linearGradient>
        <li><a href="/Team:Fudan-TSI/Judging">Judging</a></li>
+
  </defs>
    </ul></div>
+
  <rect id="rect1" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad1)"></rect>
</li>
+
  <rect id="rect2" x="0" y="0" width="1600" height="600" stroke="none" fill="url(#grad2)"></rect>
<li><div class="collapsible-header"><span>Results</span></div>
+
</svg>
    <div class="collapsible-body"><ul>
+
<div id="demoCover"><img id="coverPic" src="https://static.igem.org/mediawiki/2019/8/81/T--Fudan-TSI--coverBasicParts.gif"></div>
        <li><a href="/Team:Fudan-TSI/Results#ReverseTranscription">Reverse Transcription</a></li>
+
</div>
        <li><a href="/Team:Fudan-TSI/Results#Recombination">Recombination</a></li>
+
<style>
        <li><a href="/Team:Fudan-TSI/Demonstrate">Demonstration</a></li>
+
#pageCover{
        <li><a href="/Team:Fudan-TSI/Measurement">Measurement</a></li>
+
width:100%;
        <li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li>
+
margin:0;
    </ul></div>
+
padding-top:80px;
</li>
+
}
<li><div class="collapsible-header"><span>Model</span></div>
+
#demoCover{
    <div class="collapsible-body"><ul>
+
width:100vw;
        <li><a href="/Team:Fudan-TSI/Model">Modeling</a></li>
+
height:80vh;
        <li><a href="/Team:Fudan-TSI/Software">Software</a></li>
+
position:absolute;
        <li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li>
+
background-color:rgba(8,39,58,0.5);
    </ul></div>
+
top:70px;
</li>
+
left:0;
<li><div class="collapsible-header"><span>Parts</span></div>
+
text-align:center;
    <div class="collapsible-body"><ul>
+
}
        <li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li>
+
#coverPic{
        <li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li>
+
width:600px;
        <li><a href="/Team:Fudan-TSI/Improve">Improved parts</a></li>
+
margin:20vh auto;
        <li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li>
+
}
    </ul></div>
+
#demo{
</li>
+
width:100vw;
<li><div class="collapsible-header"><span>Outreach</span></div>
+
height:70vh;
    <div class="collapsible-body"><ul>
+
position:relative;
        <li><a href="/Team:Fudan-TSI/Public_Engagement">Education &amp; Public engagement</a></li>
+
}
        <li><a href="/Team:Fudan-TSI/Integrated_Human_Practice">Integrated human practice</a></li>
+
#demo svg {
        <li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li>
+
  width: 100%;
        <li><a href="/Team:Fudan-TSI/Safety">Safety</a></li>
+
  height: 100%;
    </ul></div>
+
  position: fixed;
</li>
+
}
<li><div class="collapsible-header"><span>Team</span></div>
+
#demo svg g {
    <div class="collapsible-body"><ul>
+
  mix-blend-mode: lighten;
        <li><a href="/Team:Fudan-TSI/Team">Members</a></li>
+
}
        <li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li>
+
#demo svg polygon {
        <li><a href="https://2018.igem.org/Team:Fudan/Heritage" target=_blank>Heritage</a></li>
+
  stroke: none;
    </ul></div>
+
  fill: white;
</li>
+
}
                    </ul><!-- .expandable -->
+
                </li>
+
@media only screen and (max-width:1100px){
                <li><div class="placeHolder"></div></li>
+
#pageCover{
            </ul>
+
padding-top:55px;
        </header>
+
}
 +
#demoCover{
 +
top:55px;
 +
height:30vh;
 +
}
 +
#demo{
 +
height:30vh;
 +
}
 +
#coverPic{
 +
width:500px;
 +
margin:7vh auto;
 +
}
 +
}
 +
@media only screen and (max-width:992px){
 +
#pageCover{
 +
padding-top:55px;
 +
}
 +
#demoCover{
 +
top:55px;
 +
}
 +
#coverPic{
 +
width:400px;
 +
margin:6vh auto;
 +
}
 +
}
 +
@media only screen and (max-width:768px){
 +
#pageCover{
 +
padding-top:55px;
 +
}
 +
#demoCover{
 +
top:55px;
 +
}
 +
#coverPic{
 +
width:350px;
 +
margin:8vh auto;
 +
}
 +
}
 +
@media only screen and (max-width:500px){
 +
#coverPic{
 +
width:250px;
 +
margin:8vh auto;
 +
}
 +
}
 +
</style>
 +
<script>
 +
//////////////////////////////
 +
// Demo Functions
 +
//////////////////////////////
 +
function bkgFunction(showStats) {
 +
  // stats
 +
  if (showStats) {
 +
var stats = new Stats();
 +
stats.domElement.style.position = 'absolute';
 +
stats.domElement.style.left = '0';
 +
stats.domElement.style.top = '0';
 +
document.body.appendChild(stats.domElement);
 +
requestAnimationFrame(function updateStats(){
 +
  stats.update();
 +
  requestAnimationFrame(updateStats);
 +
});
 +
  }
 +
  // init
 +
  var svg = document.getElementById('demo');
 +
  tesselation.setup(svg);
 +
  gradients.setup();
 +
  var lastTransitionAt, transitionDelay = 10000, transitionDuration = 3000;
 +
  function playNextTransition() {
 +
tesselation.next(transitionDuration);
 +
gradients.next(transitionDuration);
 +
  };
 +
  function tick(time) {
 +
if (!lastTransitionAt || time - lastTransitionAt > transitionDelay) {
 +
  lastTransitionAt = time;
 +
  playNextTransition();
 +
}
 +
window.requestAnimationFrame(tick);
 +
  }
 +
  window.requestAnimationFrame(tick);
 +
}
 +
//////////////////////////////
 +
// Delaunay Triangulation
 +
//////////////////////////////
 +
var calcDelaunayTriangulation = (function() {
 +
  var EPSILON = 1.0 / 1048576.0;
 +
  function getSuperT(vertices) {
 +
var xMin = Number.POSITIVE_INFINITY, yMin = Number.POSITIVE_INFINITY,
 +
xMax = Number.NEGATIVE_INFINITY, yMax = Number.NEGATIVE_INFINITY,
 +
i, xDiff, yDiff, maxDiff, xCenter, yCenter;
 +
for(i = vertices.length; i--; ) {
 +
  if(vertices[i][0] < xMin) xMin = vertices[i][0];
 +
  if(vertices[i][0] > xMax) xMax = vertices[i][0];
 +
  if(vertices[i][1] < yMin) yMin = vertices[i][1];
 +
  if(vertices[i][1] > yMax) yMax = vertices[i][1];
 +
}
 +
xDiff = xMax - xMin;
 +
yDiff = yMax - yMin;
 +
maxDiff = Math.max(xDiff, yDiff);
 +
xCenter = xMin + xDiff * 0.5;
 +
yCenter = yMin + yDiff * 0.5;
 +
return [
 +
  [xCenter - 20 * maxDiff, yCenter - maxDiff],
 +
  [xCenter, yCenter + 20 * maxDiff],
 +
  [xCenter + 20 * maxDiff, yCenter - maxDiff]
 +
];
 +
  }
 +
  function circumcircle(vertices, i, j, k) {
 +
var xI = vertices[i][0], yI = vertices[i][1],
 +
xJ = vertices[j][0], yJ = vertices[j][1],
 +
xK = vertices[k][0], yK = vertices[k][1],
 +
yDiffIJ = Math.abs(yI - yJ), yDiffJK = Math.abs(yJ - yK),
 +
xCenter, yCenter, m1, m2, xMidIJ, xMidJK, yMidIJ, yMidJK, xDiff, yDiff;
 +
// bail condition
 +
if(yDiffIJ < EPSILON){
 +
if (yDiffJK < EPSILON){
 +
throw new Error("Can't get circumcircle since all 3 points are y-aligned");
 +
}
 +
}
 +
 +
 +
// calc circumcircle center x/y, radius
 +
m1  = -((xJ - xI) / (yJ - yI));
 +
m2  = -((xK - xJ) / (yK - yJ));
 +
xMidIJ = (xI + xJ) / 2.0;
 +
xMidJK = (xJ + xK) / 2.0;
 +
yMidIJ = (yI + yJ) / 2.0;
 +
yMidJK = (yJ + yK) / 2.0;
 +
xCenter = (yDiffIJ < EPSILON) ? xMidIJ :
 +
  (yDiffJK < EPSILON) ? xMidJK :
 +
  (m1 * xMidIJ - m2 * xMidJK + yMidJK - yMidIJ) / (m1 - m2);
 +
yCenter  = (yDiffIJ > yDiffJK) ?
 +
  m1 * (xCenter - xMidIJ) + yMidIJ :
 +
  m2 * (xCenter - xMidJK) + yMidJK;
 +
xDiff = xJ - xCenter;
 +
yDiff = yJ - yCenter;
 +
// return
 +
return {i: i, j: j, k: k, x: xCenter, y: yCenter, r: xDiff * xDiff + yDiff * yDiff};
 +
  }
 +
  function dedupeEdges(edges) {
 +
var i, j, a, b, m, n;
 +
for(j = edges.length; j; ) {
 +
  b = edges[--j]; a = edges[--j];
 +
  for(i = j; i; ) {
 +
n = edges[--i]; m = edges[--i];
 +
if(a === m){
 +
  if (b===n){
 +
  edges.splice(j, 2); edges.splice(i, 2);
 +
  break;
 +
  }  
 +
}
 +
if(a === n){
 +
  if (b===m){
 +
  edges.splice(j, 2); edges.splice(i, 2);
 +
  break;
 +
  }  
 +
}
 +
  }
 +
}
 +
  }
 +
  return function(vertices) {
 +
var n = vertices.length,
 +
i, j, indices, st, candidates, locked, edges, dx, dy, a, b, c;
 +
// bail if too few / too many verts
 +
if(n < 3 || n > 2000)
 +
  return [];
 +
// copy verts and sort indices by x-position
 +
vertices = vertices.slice(0);
 +
indices = new Array(n);
 +
for(i = n; i--; )
 +
  indices[i] = i;
 +
indices.sort(function(i, j) {
 +
  return vertices[j][0] - vertices[i][0];
 +
});
 +
// supertriangle
 +
st = getSuperT(vertices);
 +
vertices.push(st[0], st[1], st[2]);
 +
// init candidates/locked tris list
 +
candidates = [circumcircle(vertices, n + 0, n + 1, n + 2)];
 +
locked = [];
 +
edges = [];
 +
// scan left to right
 +
for(i = indices.length; i--; edges.length = 0) {
 +
  c = indices[i];
 +
  // check candidates tris against point
 +
  for(j = candidates.length; j--; ) {
 +
// lock tri if point to right of circumcirc
 +
dx = vertices[c][0] - candidates[j].x;
 +
if (dx > 0.0){
 +
if(dx * dx > candidates[j].r){
 +
locked.push(candidates[j]);
 +
  candidates.splice(j, 1);
 +
  continue;
 +
}
 +
}
 +
 
 +
 
 +
// point outside circumcirc = leave candidates
 +
dy = vertices[c][1] - candidates[j].y;
 +
if(dx * dx + dy * dy - candidates[j].r > EPSILON)
 +
  continue;
 +
// point inside circumcirc = break apart, save edges
 +
edges.push(
 +
  candidates[j].i, candidates[j].j,
 +
  candidates[j].j, candidates[j].k,
 +
  candidates[j].k, candidates[j].i
 +
);
 +
candidates.splice(j, 1);
 +
  }
 +
  // new candidates from broken edges
 +
  dedupeEdges(edges);
 +
  for(j = edges.length; j; ) {
 +
b = edges[--j];
 +
a = edges[--j];
 +
candidates.push(circumcircle(vertices, a, b, c));
 +
  }
 +
}
 +
// close candidates tris, remove tris touching supertri verts
 +
for(i = candidates.length; i--; )
 +
  locked.push(candidates[i]);
 +
candidates.length = 0;
 +
for(i = locked.length; i--; )
 +
  if(locked[i].i < n){
 +
  if(locked[i].j < n){
 +
  if(locked[i].k < n){
 +
  candidates.push(locked[i].i, locked[i].j, locked[i].k);
 +
  }
 +
  }
 +
  }
 +
 +
 +
// done
 +
return candidates;
 +
  };
 +
})();
 +
var tesselation = (function() {
 +
  var svg, svgW, svgH, prevGroup;
 +
  function createRandomTesselation() {
 +
var wW = window.innerWidth;
 +
var wH = window.innerHeight;
 +
var gridSpacing = 250, scatterAmount = 0.75;
 +
var gridSize, i, x, y;
 +
if (wW / wH > svgW / svgH) { // window wider than svg = use width for gridSize
 +
  gridSize = gridSpacing * svgW / wW;
 +
} else { // window taller than svg = use height for gridSize
 +
  gridSize = gridSpacing * svgH / wH;
 +
}
 +
var vertices = [];
 +
var xOffset = (svgW % gridSize) / 2, yOffset = (svgH % gridSize) / 2;
 +
for (x = Math.floor(svgW/gridSize) + 1; x >= -1; x--) {
 +
  for (y = Math.floor(svgH/gridSize) + 1; y >= -1; y--) {
 +
vertices.push(
 +
  [
 +
xOffset + gridSize * (x + scatterAmount * (Math.random() - 0.5)),
 +
yOffset + gridSize * (y + scatterAmount * (Math.random() - 0.5))
 +
  ]
 +
);
 +
  }
 +
}
 +
var triangles = calcDelaunayTriangulation(vertices);
 +
var group = document.createElementNS('http://www.w3.org/2000/svg','g');
 +
var polygon;
 +
for(i = triangles.length; i; ) {
 +
  polygon = document.createElementNS('http://www.w3.org/2000/svg','polygon');
 +
  polygon.setAttribute('points',
 +
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
 +
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1] + ' ' +
 +
vertices[triangles[--i]][0] + ',' + vertices[triangles[i]][1]
 +
  );
 +
  group.appendChild(polygon);
 +
}
 +
return group;
 +
  }
 +
  return {
 +
setup: function(svgElement) {
 +
  svg = svgElement;
 +
  var vb = svg.getAttribute('viewBox').split(/\D/g);
 +
  svgW = vb[2];
 +
  svgH = vb[3];
 +
},
 +
next: function(t) {
 +
  var toRemove, i, n;
 +
  t /= 1000;
 +
if(prevGroup){
 +
if(prevGroup.children){
 +
if(prevGroup.children.length){
 +
toRemove = prevGroup;
 +
n = toRemove.children.length;
 +
for (i = n; i--; ) {
 +
  TweenMax.to(toRemove.children[i], t*0.4, {opacity: 0, delay: t*(0.3*i/n)});
 +
}
 +
TweenMax.delayedCall(t * (0.7 + 0.05), function(group) { svg.removeChild(group); }, [toRemove], this);
 +
}
 +
}
 +
}
 +
 +
  var g = createRandomTesselation();
 +
  n = g.children.length;
 +
  for (i = n; i--; ) {
 +
TweenMax.fromTo(g.children[i], t*0.4, {opacity: 0}, {opacity: 0.3 + 0.25 * Math.random(), delay: t*(0.3*i/n + 0.3), ease: Back.easeOut});
 +
  }
 +
  svg.appendChild(g);
 +
  prevGroup = g;
 +
}
 +
  }
 +
})();
 +
//////////////////////////////
 +
// Gradients
 +
//////////////////////////////
 +
var gradients = (function() {
 +
  var grad1, grad2, showingGrad1;
 +
  // using colors from IBM Design Colors this time
 +
  var colors = [ // 14 colors - use 3-5 span
 +
'#3c6df0', // ultramarine50
 +
'#12a3b4', // aqua40
 +
'#00a78f', // teal40
 +
'#00aa5e', // green40
 +
'#81b532', // lime30
 +
'#e3bc13', // yellow20
 +
'#ffb000', // gold20
 +
'#fe8500', // orange30
 +
'#fe6100', // peach40
 +
'#e62325', // red50
 +
'#dc267f', // magenta50
 +
'#c22dd5', // purple50
 +
'#9753e1', // violet50
 +
'#5a3ec8'  // indigo60
 +
  ];
 +
  function assignRandomColors(gradObj) {
 +
var rA = Math.floor(colors.length * Math.random());
 +
var rB = Math.floor(Math.random() * 3) + 3; // [3 - 5]
 +
rB = (rA + (rB * (Math.random() < 0.5 ? -1 : 1)) + colors.length) % colors.length;
 +
gradObj.stopA.setAttribute('stop-color', colors[rA]);
 +
gradObj.stopB.setAttribute('stop-color', colors[rB]);
 +
  }
 +
  return {
 +
setup: function() {
 +
  showingGrad1 = false;
 +
  grad1 = {
 +
stopA: document.getElementById('stop1a'),
 +
stopB: document.getElementById('stop1b'),
 +
rect:  document.getElementById('rect1')
 +
  };
 +
  grad2 = {
 +
stopA: document.getElementById('stop2a'),
 +
stopB: document.getElementById('stop2b'),
 +
rect:  document.getElementById('rect2')
 +
  };
 +
  grad1.rect.style.opacity = 0;
 +
  grad2.rect.style.opacity = 0;
 +
},
 +
next: function(t) {
 +
  t /= 1000;
 +
  var show, hide;
 +
  if (showingGrad1) {
 +
hide = grad1;
 +
show = grad2;
 +
  } else {
 +
hide = grad2;
 +
show = grad1;
 +
  }
 +
  showingGrad1 = !showingGrad1;
 +
  TweenMax.to(hide.rect, 0.55*t, {opacity: 0, delay: 0.2*t, ease: Sine.easeOut});
 +
  assignRandomColors(show);
 +
  TweenMax.to(show.rect, 0.65*t, {opacity: 1, ease: Sine.easeIn});
 +
}
 +
  };
 +
})();
 +
//////////////////////////////
 +
// Start
 +
//////////////////////////////
 +
bkgFunction();
 +
</script>
 +
  
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                        <defs>
 
                            <linearGradient y2="0%" x2="100%" y1="0%" x1="0%" id="blackgraleft">
 
                                <stop stop-color="rgb(0,0,0)" stop-opacity="1" offset="0%"/>
 
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                            </linearGradient>
 
                        </defs>
 
                        <g>
 
                            <rect id="svg_1" fill="url(#blackgraleft)" height="100%" width="100%"/>
 
                        </g>
 
                    </svg>
 
                    <svg width="10" height="10" xmlns="http://www.w3.org/2000/svg" style="position:absolute; right:0;top:0; width: 4%;height: 100%;">
 
                        <defs>
 
                            <linearGradient y2="0%" x2="100%" y1="0%" x1="0%" id="blackgraright">
 
                                <stop stop-color="rgb(0,0,0)" stop-opacity="0" offset="0%"/>
 
                                <stop stop-color="rgb(0,0,0)" stop-opacity="1" offset="100%"/>
 
                            </linearGradient>
 
                        </defs>
 
                        <g>
 
                            <rect id="svg_2" fill="url(#blackgraright)" height="100%" width="100%"/>
 
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                        <h2>text from greatbay</h2>
 
                        <p class="flow-text">
 
Parts Overview
 
with 19 composite parts, and 36 basic parts. Don't think we win at quantity but we actually provided a series of data with high-quality. In the production of geraniol in E.coli, our favourite basic part is BBa_K2753003, pSB1C3-obGES cds. We have also created and synthesized competent pathways in yeast to convert geraniol to neptalactol. Besides, based on previous part like BBa_J23119, we generated a stabilised version by adding an up-element, a transcription-activator-like effector(TALE) binding site, and a genetic circuit encoding for TALE2. In addition, We created a novel, well-characterized, and well-documented TALE stabilized promoter (TALEsp) collection as well as two parts enabling future teams to create more stabilized promoters. This promoter library provides a reliable tool which allows teams to construct predictable and robust synthetic systems. Check the GreatBay_China's parts family!
 
Part Table
 
  
      Name  Type  Description Designer  Length
+
    W BBa_K2753002  Coding  pSB1C3-GPPS cds WEI KUANGYI  894
+
<div id="pageContent">
    W BBa_K2753003  Coding  pSB1C3-obGES cds  WEI KUANGYI  1704
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
    W BBa_K2753018  Composite TALE2 sp1 WEI KUANGYI  3166
+
<!---- Content ---->
    W BBa_K2753023  Composite TALE2 sp6 WEI KUANGYI  3164
+
<!----------------------------------------------------------------------------------------------------------------------------------------->
    W BBa_K2753030  Composite TALE1 sp1 WEI KUANGYI  3166
+
    W BBa_K2753010  Composite pSB1C3-TDH3 promoter-G8H-tENO2  WEI KUANGYI  2374
+
<div class="container">
    W BBa_K2753011  Composite pSB1C3-TEF1 promoter-GOR-tENO1  WEI KUANGYI  1787
+
<div class="row">
    W BBa_K2753012  Composite pSB1C3-pRPL18P promoter-CrISY-tTDH1 WEI KUANGYI  2127
+
    W BBa_K2753013  Composite pSB1C3-pTALE1-CP  WEI KUANGYI  3956
+
<div class="row title1" id="mainTitle1">
    W BBa_K2753014  Composite pSB1C3-pTALE2-CP  WEI KUANGYI  3956
+
<div class="col">Parts Overview</div>
    W BBa_K2753015  Composite pSB1C3-RiboJ-rbs-GPPS-rbs-ObGES WEI KUANGYI  2781
+
</div>
    W BBa_K2753016  Composite pSB1C3-RiboJ-rbs-GPPS-GES-double terminator WEI KUANGYI  4028
+
    W BBa_K2753019  Composite TALE2 sp2 WEI KUANGYI  3166
+
<div class="row para1">
    W BBa_K2753020  Composite TALE2 sp3 WEI KUANGYI  3166
+
<div class="row">
    W BBa_K2753021  Composite TALE2 sp4 WEI KUANGYI  3160
+
<div class="col">
    W BBa_K2753022  Composite TALE2 sp5 WEI KUANGYI  3160
+
This year Team Fudan-TSI presents you with a toolbox for our in vivo sequence-specific continuous mutagenesis system(R-Evolution). This toolbox includes promoters, RBSs, terminators, CDSs and other regulatory parts which undergo careful design and optimization. The Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT), which is new to the registry, and Cre Recombinase are the most important components of our system for which we have put emphasis on the construction and characterization of these two parts. Also, our improved lac repressor protein is fully equipped for regulating protein expression.
    W BBa_K2753024  Composite TALE1 sp2 WEI KUANGYI  3160
+
</div>
    W BBa_K2753025  Composite TALE1 sp3 WEI KUANGYI  3166
+
</div>
    W BBa_K2753026  Composite TALE1 sp4 WEI KUANGYI  3160
+
</div>
    W BBa_K2753027  Composite TALE1 sp5 WEI KUANGYI  3160
+
    W BBa_K2753029  Composite TALE1 sp6 WEI KUANGYI  3160
+
<div class="row title2" id="mainTitle2">
  U W BBa_K2753032  Terminator  L3S2P21 WEI KUANGYI  60
+
<div class="col">References</div>
  U W BBa_K2753033  Regulatory  PT7A1w2 WEI KUANGYI  69
+
</div>
  U W BBa_K2753034  Regulatory  PT7A1w3 WEI KUANGYI  69
+
<div class="row para1">
  U W BBa_K2753035  Tag SarJ  WEI KUANGYI  79
+
<div class="col">
  U W BBa_K2753036  RBS RBSsp1  WEI KUANGYI  20
+
<div class="col">
  U W BBa_K2753037  RBS RBSsp2  WEI KUANGYI  20
+
<ul class="paraUl" style="list-style:none;">
  U W BBa_K2753038  Coding  TALE1 WEI KUANGYI  2640
+
<li> [1] Wen-Hui Zhang, Evguenia S. Svarovskaia, Rebekah Barr, and Vinay K. Pathak. Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine–thymine tracts[J]. Proc Natl Acad Sci U S A. 2002 Jul 23; 99(15): 10090–10095</li>
  U W BBa_K2753039  Coding  TALEsp2 WEI KUANGYI  2640
+
</ul>
  U W BBa_K2753043  Regulatory  promoter sp1  WEI KUANGYI  79
+
</div>
  U W BBa_K2753044  Regulatory  TDH3 promoter WEI KUANGYI  667
+
</div>
  U W BBa_K2753045  Regulatory  TEF1 promoter WEI KUANGYI  408
+
</div>
  U W BBa_K2753046  Regulatory  pRPL18P promoter  WEI KUANGYI  709
+
  U W BBa_K2753047  Coding  G8H WEI KUANGYI  1482
+
</div>
  U W BBa_K2753048  Coding  GOR WEI KUANGYI  1137
+
</div>
  U W BBa_K2753049  Coding  CrISY WEI KUANGYI  1167
+
  U W BBa_K2753050  Terminator  tENO2 WEI KUANGYI  225
+
  U W BBa_K2753051  Terminator  tENO1 WEI KUANGYI  242
+
  U W BBa_K2753052  Terminator  tTDH1 WEI KUANGYI  251
+
  U W BBa_K2753053  RBS RBStrGPPS WEI KUANGYI  56
+
  U W BBa_K2753054  RBS Charles RBS WEI KUANGYI  14
+
  U W BBa_K2753055  Regulatory  UPJ23119  WEI KUANGYI  55
+
  U W BBa_K2753056  Regulatory  promoter sp2  WEI KUANGYI  79
+
  U W BBa_K2753057  Regulatory  PUPAsp2 WEI KUANGYI  79
+
  U W BBa_K2753058  Regulatory  PUPAAsp2  WEI KUANGYI  79
+
  U W BBa_K2753059  Regulatory  PDNAsp2 WEI KUANGYI  73
+
  U W BBa_K2753060  Regulatory  PDNAsp2w  WEI KUANGYI  73
+
  U W BBa_K2753061  Regulatory  SP119 WEI KUANGYI  77
+
  U W BBa_K2753062  Regulatory  Psp1 mutated  WEI KUANGYI  73
+
  U W BBa_K2753063  Regulatory  PUPsp1 mutated  WEI KUANGYI  79
+
  U W BBa_K2753064  Regulatory  PDNsp1  WEI KUANGYI  73
+
  U W BBa_K2753065  Regulatory  PDNsp1w WEI KUANGYI  73
+
  U W BBa_K2753066  Regulatory  Psp1  WEI KUANGYI  73
+
  U W BBa_K2753067  Regulatory  Psp1w WEI KUANGYI  73
+
  U W BBa_K2753070  RBS RBSobGES  WEI KUANGYI  24
+
  
   
+
     
+
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<h3 class="col s12" style="text-align: left; color: rgba(255, 255, 255, 0.8); font-size:12.5px; margin-top:5px;">R-Evolution: an <i>in vivo</i> sequence-specific toolbox for continuous mutagenesis</h3>
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                    <p class="flow-text" style="margin:0">Mutation library generation is critical for biological and medical research, but current methods cannot mutate a specific sequence continuously without manual intervention. Here we present a toolbox for <i>in vivo</i> continuous mutation library construction. First, the target DNA is transcribed into RNA. Next, our reverse transcriptase reverts RNA into cDNA, during which the target is randomly mutated by enhanced error-prone reverse transcription. Finally, the mutated version replaces the original sequence through recombination. These steps will be carried out iteratively, generating a random mutation library of the target with high efficiency as mutations accumulate along with bacterial growth. Our toolbox is orthogonal and provides a wide range of applications among various species. R-Evolution could mutate coding sequences and regulatory sequences, which enables the <i>in vivo</i> evolution of individual proteins or multiple targets at a time, promotes high-throughput research, and serves as a foundational advance to synthetic biology.
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Revision as of 06:09, 18 October 2019

Parts Overview
This year Team Fudan-TSI presents you with a toolbox for our in vivo sequence-specific continuous mutagenesis system(R-Evolution). This toolbox includes promoters, RBSs, terminators, CDSs and other regulatory parts which undergo careful design and optimization. The Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT), which is new to the registry, and Cre Recombinase are the most important components of our system for which we have put emphasis on the construction and characterization of these two parts. Also, our improved lac repressor protein is fully equipped for regulating protein expression.
References
  • [1] Wen-Hui Zhang, Evguenia S. Svarovskaia, Rebekah Barr, and Vinay K. Pathak. Y586F mutation in murine leukemia virus reverse transcriptase decreases fidelity of DNA synthesis in regions associated with adenine–thymine tracts[J]. Proc Natl Acad Sci U S A. 2002 Jul 23; 99(15): 10090–10095