Difference between revisions of "Team:ShanghaiFLS China/Basic Part"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2019.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2019.igem.org/Judging/Awards"> award listed below</a>. </p>
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2019.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<h1>Basic Parts</h1>
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<p>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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</p>
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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<h3>Note</h3>
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<p>This page should list all the basic parts your team has made during your project and include direct links to your Parts main pages on the Registry. <b>You must add all characterization information for your parts on Parts Main Page on the Registry.</b> You should <b>not</b> put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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<h3>Best Basic Part Special Prize</h3>
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<p> To be eligible for this award, this part <b>must be well documented on the part's Main Page on the Registry</b>. If you have a part you wish to nominate your team for this <a href="https://2019.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2019.igem.org/Judging/Judging_Form">judging form</a> and delete the alert box at the top of this page.
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<br><br>
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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                    <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Parts">Parts Overview</a></li>
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                    <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Basic_Part">Basic Parts</a></li>
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                    <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Composite_Part">Composite Parts</a></li>
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                    <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Part_Collection">Part Collection</a></li>
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                    <h2 class="editContent">Part Collection</h2>
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                    <p>Our parts range from BBa_K3239000 to BBa_K3239011. Among them the basic parts are the PRM1 promoter (pRRM1), the MIT1 promoter (pMIT1), the AOX1 promoter (pAOX1), the PRM1 gene (transcription factor for pAOX1, pPRM1 and pMIT1), the MIT1 gene (transcription factor for pAOX1 and pPRM1), and the yEGFP3 gene, all of them homogenous to the <em>P. pastoris</em> GS115 strain except for yEGFP3. The composite parts are constructed from these parts and are respectively pAOX1-GFP, pMIT1-PRM1, pPRM1-MIT1 and pMIT1-PRM1-pPRM1-MIT1. All of these composite parts are to be reintegrated into wild type <em>P. pastoris</em> GS115 (for pMIT1-PRM1-pPRM1-MIT1, it is the integration of pMIT1-PRM1 and pPRM1-MIT1 separately). In the strain containing only AOX1-GFP, yEGFP3 is the reporter gene that reflects AOX1 activity. The rest three composite parts shall each be integrated into the AOX1-GFP strain, so that the up regulation of pAOX1 can be compared with the wlid type. Up-regulation is achieved via the recombination of the homogenous transcription factors and promoters so that the transcription factors are dynamically over-expressed.</p>
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                    <a href="http://parts.igem.org/Part:BBa_K3239000">BBa_K3239000</a>
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                    <a href="http://parts.igem.org/Part:BBa_K3239002">BBa_K3239002</a>
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                    <a href="http://parts.igem.org/Part:BBa_K3239003">BBa_K3239003</a>
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                    <a href="http://parts.igem.org/Part:BBa_K3239004">BBa_K3239004</a>
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                    <a href="http://parts.igem.org/Part:BBa_K3239005">BBa_K3239005</a>
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                    <a href="http://parts.igem.org/Part:BBa_K3239006">BBa_K3239006</a>
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Revision as of 15:40, 17 October 2019


ShanghaiFLS_China: Basic Parts