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− | + | {{ShanghaiFLS_China/bootstrap.css}} | |
− | + | {{ShanghaiFLS_China/font-awesome.min.css}} | |
− | + | {{ShanghaiFLS_China/style_bg.css}} | |
− | + | {{ShanghaiFLS_China/important.css}} | |
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+ | <html lang="en"> | ||
+ | <head> | ||
+ | <title>ShanghaiFLS_China: Basic Parts</title> | ||
+ | |||
+ | <!-- Meta tag Keywords --> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
+ | <meta charset="utf-8"> | ||
+ | <meta name="Description" content="iGEM 2019 ShanghaiFLS_China team members"> | ||
+ | <!-- scroll top script when refresh a page | ||
+ | <script type="application/x-javascript"> | ||
+ | addEventListener("load", function () { | ||
+ | setTimeout(hideURLbar, 0); | ||
+ | }, false); | ||
+ | |||
+ | function hideURLbar() { | ||
+ | window.scrollTo(0, 1); | ||
+ | } | ||
+ | </script> | ||
+ | <!--// Meta tag Keywords --> | ||
+ | |||
+ | </head> | ||
+ | |||
+ | <body> | ||
+ | <!-- //header --> | ||
+ | <header> | ||
+ | <div class="container" id="home"> | ||
+ | <!-- nav --> | ||
+ | <nav class="py-4"> | ||
+ | <div id="logo"> | ||
+ | <h1> | ||
+ | <a href="https://2019.igem.org/Team:ShanghaiFLS_China">Team:ShanghaiFLS_China</a> | ||
+ | </h1> | ||
+ | </div> | ||
+ | |||
+ | <label for="drop" class="toggle"></label> | ||
+ | <input type="checkbox" id="drop" /> | ||
+ | <ul class="menu mt-2"> | ||
+ | <li class="mr-lg-4 mr-3 active"><a href="https://2019.igem.org/Team:ShanghaiFLS_China">Home</a></li> | ||
+ | <li class="mr-lg-4 mr-3"> | ||
+ | <label for="drop-2" class="toggle">Team<span class="fa fa-angle-down" aria-hidden="true"></span> </label> | ||
+ | <a>Team<span class="fa fa-angle-down" aria-hidden="true"></span></a> | ||
+ | <input type="checkbox" id="drop-2"/> | ||
+ | <ul> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Team">Team</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Collaborations">Collaborations</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="mr-lg-4 mr-3"> | ||
+ | <label for="drop-2" class="toggle">Project<span class="fa fa-angle-down" aria-hidden="true"></span> </label> | ||
+ | <a>Project<span class="fa fa-angle-down" aria-hidden="true"></span></a> | ||
+ | <input type="checkbox" id="drop-2"/> | ||
+ | <ul> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Description">Description</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Design">Design</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Experiments">Experiments</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Notebook">Notebook</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Contribution">Contribution</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Results">Results</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Model">Model</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Demonstrate">Demonstrate</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Attributions">Attributions</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="mr-lg-4 mr-3 active"> | ||
+ | <label for="drop-2" class="toggle">Parts<span class="fa fa-angle-down" aria-hidden="true"></span> </label> | ||
+ | <a>Parts<span class="fa fa-angle-down" aria-hidden="true"></span></a> | ||
+ | <input type="checkbox" id="drop-2"/> | ||
+ | <ul> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Parts">Parts Overview</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Basic_Part">Basic Parts</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Composite_Part">Composite Parts</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Part_Collection">Part Collection</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="mr-lg-4 mr-3"><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Safety">Safety</a></li> | ||
+ | <li class="mr-lg-4 mr-3"> | ||
+ | <label for="drop-2" class="toggle">Human Practices<span class="fa fa-angle-down" aria-hidden="true"></span> </label> | ||
+ | <a>Human Practices<span class="fa fa-angle-down" aria-hidden="true"></span></a> | ||
+ | <input type="checkbox" id="drop-2"/> | ||
+ | <ul> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Human_Practices">Integrated Human Practices</a></li> | ||
+ | <li><a href="https://2019.igem.org/Team:ShanghaiFLS_China/Public_Engagement">Education and Engagement</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </nav> | ||
+ | <!-- //nav --> | ||
+ | </div> | ||
+ | </header> | ||
+ | <!-- //header --> | ||
+ | |||
+ | <!-- banner --> | ||
+ | <section class="banner"> | ||
+ | <div class="banner-layer"> | ||
+ | <div class="container"> | ||
+ | <div class="row w3pvt_banner_info"> | ||
+ | <div class="col-md-8 w3layouts_banner_margin"> | ||
+ | <h2 class="editContent">Part Collection</h2> | ||
+ | <p>Our parts range from BBa_K3239000 to BBa_K3239011. Among them the basic parts are the PRM1 promoter (pRRM1), the MIT1 promoter (pMIT1), the AOX1 promoter (pAOX1), the PRM1 gene (transcription factor for pAOX1, pPRM1 and pMIT1), the MIT1 gene (transcription factor for pAOX1 and pPRM1), and the yEGFP3 gene, all of them homogenous to the <em>P. pastoris</em> GS115 strain except for yEGFP3. The composite parts are constructed from these parts and are respectively pAOX1-GFP, pMIT1-PRM1, pPRM1-MIT1 and pMIT1-PRM1-pPRM1-MIT1. All of these composite parts are to be reintegrated into wild type <em>P. pastoris</em> GS115 (for pMIT1-PRM1-pPRM1-MIT1, it is the integration of pMIT1-PRM1 and pPRM1-MIT1 separately). In the strain containing only AOX1-GFP, yEGFP3 is the reporter gene that reflects AOX1 activity. The rest three composite parts shall each be integrated into the AOX1-GFP strain, so that the up regulation of pAOX1 can be compared with the wlid type. Up-regulation is achieved via the recombination of the homogenous transcription factors and promoters so that the transcription factors are dynamically over-expressed.</p> | ||
+ | </div> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K3239000">BBa_K3239000</a> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K3239002">BBa_K3239002</a> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K3239003">BBa_K3239003</a> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K3239004">BBa_K3239004</a> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K3239005">BBa_K3239005</a> | ||
+ | <a href="http://parts.igem.org/Part:BBa_K3239006">BBa_K3239006</a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </section> | ||
+ | <!-- //banner --> | ||
+ | <!-- footer --> | ||
+ | <footer class="py-5"> | ||
+ | <div class="container"> | ||
+ | <div class="w3layoutsinfo-grids"> | ||
+ | <div class="w3layouts-grid-left w3layouts-grid-right text-center"> | ||
+ | <div class="social mb-4"> | ||
+ | <ul> | ||
+ | <li><a><span class="fa qmr-2 fa-twitter"></span></a><a>igem2019_sfls</a></li> | ||
+ | <li><a><span class="fa qmr-2 fa-instagram"></span></a><a>igem_shanghaifls_china</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="copyright text-center"> | ||
+ | <p>Copyright © iGEM 2019, team: ShanghaiFLS_China. All rights reserved. Email: SFLS_x_iGEM@outlook.com</p> | ||
+ | <a href="#" class="move-top scroll"><span class="fa fa-angle-up" aria-hidden="true"></span></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </footer> | ||
+ | <!-- //footer --> | ||
+ | |||
+ | </body> | ||
</html> | </html> |
Revision as of 15:40, 17 October 2019