Difference between revisions of "Team:NCKU Tainan/Demonstrate"

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<h1>Demonstrate</h1>
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<h3>Gold Medal Criterion #4</h3>
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Teams that can show their system working under real world conditions are usually good at impressing the judges in iGEM. To achieve gold medal criterion #4, convince the judges that your engineered system works.  
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Your engineered system has to work under realistic conditions. Your system must comply with all <a href="https://2019.igem.org/Safety/Rules">rules</a> and <a href="https://2019.igem.org/Safety/Policies">policies</a> approved by the iGEM Safety Committee. Your system can derive from or make functional a previous iGEM project by your team or by another team. For multi-component projects, the judges may consider the function of individual components.
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                        <a class="nav-link" href="#Subtitle1">Blood p-Cresol Reader</a>
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To be eligible for this award, you must add clear documentation to this page and delete the alert box at the top of this page.
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                        <a class="nav-link" href="#Subtitle2">Alternative Tyrosine Pathway</a>
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Please see the <a href="https://2019.igem.org/Judging/Medals">2019 Medals Page</a> for more information.
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                    <h1>Demonstrate</h1>
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                    <p>    After months of hard work and effort, iGEM NCKU Tainan 2019 is proud to reveal that ‘Oh My Gut’ is now complete! Our team has spared no effort in giving their all to ensure that our project goes smoothly. With this project, we hope to achieve our vision of providing a comprehensive solution to chronic kidney disease, and also spreading awareness on the significance of p-Cresol on human health.</p>
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                    <h2 id ="Subtitle1">Blood p-Cresol Reader</h2>
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                    <p>    Our device, CreSense consists of three major parts: a centrifugal platform for blood plasma separation, a fluorescence intensity reader and a WiFi-module to monitor the fluorescence intensity online. In this section, we will demonstrate how our blood p-Cresol reader works.</p>
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                    <p>    Whole blood is injected into our <a href="https://2019.igem.org/Team:NCKU_Tainan/Hardware#Subtitle5" target="_blank">microfluidic chip</a>. All the user needs to do is to press a button to start the blood centrifugation. After approximately 15 minutes, the blood will be separated into two layers. The blood plasma will flow into the reaction chamber and react with our p-Cresol sensing bacteria. Then, the <a href="https://2019.igem.org/Team:NCKU_Tainan/Hardware#Database" target="_blank">reader</a> will show the real time fluorescence intensity reading on the LCD screen and simultaneously send it to an <a href="https://2019.igem.org/Team:NCKU_Tainan/Hardware#Database" target="_blank">online database</a>.</p>
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                                    <a href="https://static.igem.org/mediawiki/2019/thumb/3/31/T--NCKU_Tainan--Demonstration_Blood_Injection.png/800px-T--NCKU_Tainan--Demonstration_Blood_Injection.png" target="_blank" style="width:70%"><img src="https://static.igem.org/mediawiki/2019/thumb/3/31/T--NCKU_Tainan--Demonstration_Blood_Injection.png/800px-T--NCKU_Tainan--Demonstration_Blood_Injection.png" alt="" title="" style="width:100%"></a>
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                                    <figcaption>Fig1. Inject blood into centrifugal platform.</figcaption>
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                                    <a href="https://static.igem.org/mediawiki/2019/thumb/7/7f/T--NCKU_Tainan--Demonstration_Centrifugal_Platform.png/800px-T--NCKU_Tainan--Demonstration_Centrifugal_Platform.png" target="_blank" style="width:70%"><img src="https://static.igem.org/mediawiki/2019/thumb/7/7f/T--NCKU_Tainan--Demonstration_Centrifugal_Platform.png/800px-T--NCKU_Tainan--Demonstration_Centrifugal_Platform.png" alt="" title="" style="width:100%"></a>
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                                    <figcaption>Fig2. Centrifugal platform.</figcaption>
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                                    <a href="https://static.igem.org/mediawiki/2019/thumb/a/a5/T--NCKU_Tainan--Demonstration_Blood_Separation.png/800px-T--NCKU_Tainan--Demonstration_Blood_Separation.png" target="_blank" style="width:90%"><img src="https://static.igem.org/mediawiki/2019/thumb/a/a5/T--NCKU_Tainan--Demonstration_Blood_Separation.png/800px-T--NCKU_Tainan--Demonstration_Blood_Separation.png" alt="" title="" style="width:100%"></a>
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                                    <figcaption>Fig3. Separation of blood plasma into reaction chamber.</figcaption>
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                                    <a href="https://static.igem.org/mediawiki/2019/thumb/a/a7/T--NCKU_Tainan--Demonstration_Sensing_Bacteria.png/800px-T--NCKU_Tainan--Demonstration_Sensing_Bacteria.png" target="_blank" style="width:90%"><img src="https://static.igem.org/mediawiki/2019/thumb/a/a7/T--NCKU_Tainan--Demonstration_Sensing_Bacteria.png/800px-T--NCKU_Tainan--Demonstration_Sensing_Bacteria.png" alt="" title="" style="width:100%"></a>
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                                    <figcaption>Fig4. Inject p-Cresol sensing bacteria into the reaction chamber.</figcaption>
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                                    <figcaption>Fig5. Measure the fluorescence emission using a light sensor.</figcaption>
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                                    <a href="https://static.igem.org/mediawiki/2019/thumb/0/0a/T--NCKU_Tainan--Demonstration_Realtime_Database.png/800px-T--NCKU_Tainan--Demonstration_Realtime_Database.png" target="_blank" style="width:85%"><img src="https://static.igem.org/mediawiki/2019/thumb/0/0a/T--NCKU_Tainan--Demonstration_Realtime_Database.png/800px-T--NCKU_Tainan--Demonstration_Realtime_Database.png" alt="" title="" style="width:100%"></a>
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                                    <figcaption>Fig6. Send the measurement result to our online realtime database.</figcaption>
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                    <p>    This device is meant to be placed in the diagnostic center and other healthcare service providers. However, this device is not limited to just patients who take our live therapeutic. Patients who are concerned about their health or is at a high risk of developing chronic kidney disease may also use this device as a preventative measure.</p>
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                    <p>    To demonstrate our engineered system works, we have made a video to explain each part of CreSense and the operating process.</p>
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                    <video id="Logo-video" autoplay preload muted poster="https://static.igem.org/mediawiki/2019/5/58/T--NCKU_Tainan--Home_logo_poster.jpg">
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                    <h2 id ="Subtitle2">Alternative Tyrosine Fermentation Pathway</h2>
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                    <p>    iGEM NCKU Tainan 2019 engineered E. coli Nissle and introduced an alternative pathway for tyrosine in the gut by adding <a href="https://2019.igem.org/Team:NCKU_Tainan/Design#TAL" target="_blank">Tyrosine Ammonia Lyase</a>. It converts tyrosine into a beneficial product, p-Coumaric acid. In the co-culture system with Clostridioides difficile that is known to fermentate tyrosine into p-Cresol, we hope to see that our engineered E.coli Nissle can reduce the total p-Cresol outcome by preventing tyrosine fermentation into p-Cresol. This way, we can demonstrate the reduction of p-Cresol production in the gut. For more information, please refer to our <a href="https://2019.igem.org/Team:NCKU_Tainan/Design" target="_blank">Design</a> and <a href="https://2019.igem.org/Team:NCKU_Tainan/Results" target="_blank">Results</a> pages.</p>
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                    <h2>References</h2>
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Revision as of 16:12, 16 October 2019

>

Demonstrate

After months of hard work and effort, iGEM NCKU Tainan 2019 is proud to reveal that ‘Oh My Gut’ is now complete! Our team has spared no effort in giving their all to ensure that our project goes smoothly. With this project, we hope to achieve our vision of providing a comprehensive solution to chronic kidney disease, and also spreading awareness on the significance of p-Cresol on human health.

Blood p-Cresol Reader

Our device, CreSense consists of three major parts: a centrifugal platform for blood plasma separation, a fluorescence intensity reader and a WiFi-module to monitor the fluorescence intensity online. In this section, we will demonstrate how our blood p-Cresol reader works.

Whole blood is injected into our microfluidic chip. All the user needs to do is to press a button to start the blood centrifugation. After approximately 15 minutes, the blood will be separated into two layers. The blood plasma will flow into the reaction chamber and react with our p-Cresol sensing bacteria. Then, the reader will show the real time fluorescence intensity reading on the LCD screen and simultaneously send it to an online database.

Fig1. Inject blood into centrifugal platform.
Fig2. Centrifugal platform.
Fig3. Separation of blood plasma into reaction chamber.
Fig4. Inject p-Cresol sensing bacteria into the reaction chamber.
Fig5. Measure the fluorescence emission using a light sensor.
Fig6. Send the measurement result to our online realtime database.

This device is meant to be placed in the diagnostic center and other healthcare service providers. However, this device is not limited to just patients who take our live therapeutic. Patients who are concerned about their health or is at a high risk of developing chronic kidney disease may also use this device as a preventative measure.

To demonstrate our engineered system works, we have made a video to explain each part of CreSense and the operating process.

Alternative Tyrosine Fermentation Pathway

iGEM NCKU Tainan 2019 engineered E. coli Nissle and introduced an alternative pathway for tyrosine in the gut by adding Tyrosine Ammonia Lyase. It converts tyrosine into a beneficial product, p-Coumaric acid. In the co-culture system with Clostridioides difficile that is known to fermentate tyrosine into p-Cresol, we hope to see that our engineered E.coli Nissle can reduce the total p-Cresol outcome by preventing tyrosine fermentation into p-Cresol. This way, we can demonstrate the reduction of p-Cresol production in the gut. For more information, please refer to our Design and Results pages.

References

e-mail : igem.ncku.tainan@gmail.com
phone : 06-2757575