Team:NCKU Tainan/Parts
Parts
Overview
BioBricks have always been the foundational building blocks for iGEM projects. When constructing our BioBricks, we made use of the IDT synthesis offer to generate our required parts in a fast and efficient way. Additionally, we made use of PCR amplification with our constructed expression plasmids as a template to construct several BioBricks. All of the parts contain a coding sequence, some of which extended with promoters or introns. We created a part collection comprised of 14 basic parts and 10 improved composite parts. All parts were generated during our project.
Basic parts
| Name | Description | Type | Length |
|---|---|---|---|
| BBa_J23100 | Constitutive promoter (PJ23100) | Promotor | 35bp |
| BBa_K1123000 | FNR promoter (Pfnr) | Promoter | 120bp |
| BBa_ I742146 | Tyrosine ammonia-lyase native RBS (NRBS) | RBS | 14bp |
| BBa_B0017 | Double terminator (B0010-B0010) | Terminator | 160bp |
| BBa_K2997000 | Tyrosine transporter (tyrP) | Coding | 1297bp |
| BBa_I742142 | Tyrosine ammonia-lyase (sam8) | Coding | 1536bp |
| BBa_K2997001 | Bacteriocin Full length (CBM-B) | Coding | 867bp |
| BBa_K2997002 | Bacteriocin short length (sCBM-B) | Coding | 252bp |
| BBa_K2997003 | Sigma 54-dependent transcriptional regulator (pchR) | Coding | 1854bp |
| BBa_K2997004 | pchR binding range | Coding | 567bp |
| BBa_J18921 | 6aa [GS]x linker | Coding | 18bp |
| BBa_K1610300 | Secretion tag (yebF) | Coding | 354bp |
| BBa_E0040 | Wild type green fluorescence protein (wtgfp) | Coding | 720bp |
| BBa_K2762017 | Superfolder green fluorescence protein (sfgfp) | Coding | 720bp |
Composite Parts
| Name | Description | Type | Length |
|---|---|---|---|
| BBa_K2997005 | K880005 - yebF - GS linker - sCBM-B | Composite | 685bp |
| BBa_K2997006 | K880005 - yebF- TB linker - sCBM-B | 120bp | |
| BBa_K2997007 | K880005 - yebF - CBM-B | 1282bp | |
| BBa_K2997008 | Native promoter - pchR - sfgfp | 3137bp | |
| BBa_K2997009 | J23100 - NRBS - sam8 | 1606bp | |
| BBa_K2997010 | K880005 - sam8 | 1613bp | |
| BBa_K2997011 | B0034 - sam8 | 1572bp | |
| BBa_K2997012 | Pfnr-B0034-GFP | 866bp | |
| BBa_I742106 | sam8 with ribosome binding site | 1550bp | |
| BBa_K880005 | Strong promoter J23100 and strong RBS B0034 | Promoter+RBS | 55bp |
