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Previous studies have reported that the interactions between tRNA primer and mRNA template as well as the reverse transcriptase are crucial in directing subsequent cDNA synthesis <a>(James E. Dahlberg et al.)</a>. Specifically, according to the model for reverse transcription proposed by Kulpa et al., reverse transcription includes 5 steps (Fig. 1), in which the annealing of tRNA primer to the primer binding site (PBS) region on mRNA template is crucial for the synthesis of minus strand strong stop DNA (–ssDNA) and the following cDNA synthesis process. <br /><br /> | Previous studies have reported that the interactions between tRNA primer and mRNA template as well as the reverse transcriptase are crucial in directing subsequent cDNA synthesis <a>(James E. Dahlberg et al.)</a>. Specifically, according to the model for reverse transcription proposed by Kulpa et al., reverse transcription includes 5 steps (Fig. 1), in which the annealing of tRNA primer to the primer binding site (PBS) region on mRNA template is crucial for the synthesis of minus strand strong stop DNA (–ssDNA) and the following cDNA synthesis process. <br /><br /> | ||
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Many researchers have studied the reverse transcription process in viruses, from which we find two critical properties in the annealing process of tRNA primer and PBS that should be taken into consideration when building the tRNA primer designer. <br /><br /> | Many researchers have studied the reverse transcription process in viruses, from which we find two critical properties in the annealing process of tRNA primer and PBS that should be taken into consideration when building the tRNA primer designer. <br /><br /> | ||
The first property is that the 3'-terminal of the tRNA primer should be complementary to the PBS on mRNA template <a>(Kosloff et al.)</a>(Kosloff et al.). The second one is that different viruses prefer specific type of tRNA primer for reverse transcription <a>(Kulpa et al.,</a><a> Kosloff et al.)</a>. What should also be noted is that for different viruses, the lengths of PBS as well as the types of tRNA primer are different. The PBS lengths and the preferred tRNA types of 3 most well-studied retroviruses are listed in Table I.<br /><br /> | The first property is that the 3'-terminal of the tRNA primer should be complementary to the PBS on mRNA template <a>(Kosloff et al.)</a>(Kosloff et al.). The second one is that different viruses prefer specific type of tRNA primer for reverse transcription <a>(Kulpa et al.,</a><a> Kosloff et al.)</a>. What should also be noted is that for different viruses, the lengths of PBS as well as the types of tRNA primer are different. The PBS lengths and the preferred tRNA types of 3 most well-studied retroviruses are listed in Table I.<br /><br /> |
Revision as of 04:19, 16 October 2019