Difference between revisions of "Team:Guelph/Collaborations"

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<h1 class="sub1">Collaboration 1 - TEAM NAME</h1>
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<h1 class="sub1">Collaboration 1 - Ontario iGEM Meet-Up</h1>
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<p class="para1">Description of collab, add pictures below if available. </p>
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<p class="para1">As a collaboration with all other Ontario iGEM teams, we hosted a meetup at the University of Guelph. iGEM Teams presented their project, and were judged and given feedback in order to prepare for the Giant Jamboree. At this meetup, there was a discussion with Ontario Genomics about the sustainability of iGEM teams and how to ensure students can continue to take part in synthetic biology and iGEM with the high price of the competition. This was a great opportunity for all iGEMers from Ontario to voice their opinion and share how their team operates.
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<h1 class="sub1">Collaboration 2 - TEAM NAME</h1>
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<h1 class="sub1">Collaboration 2 - Queens iGEM Project Trouble-Shooting and Part Characterization
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<p class="para1">iGEM Guelph's Nicole assisted Queens iGEM with troubleshooting their protein expression. Recombinant antibody expression in <i>E. coli</i> is notoriously difficult and Queens was unable to express their BioBrick in BL21 cells over an extended period of time. Nicole made suggestions be able to better express their antibodies including: altering buffer conditions, changing expression conditions, altering the purification system, and trying a different cell line. This ultimately lead to Queens iGEM changing cell lines since their protein was more likely to fold in a cell line that contains an oxidizing cytoplasmic environment.
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Additionally, we helped Queens iGEM develop their Bronze Medal characterization experiments for parts BBa_K608010 and BBa_I746909. One of the suggestions we made  for improving their protein yield was trying constitutive promoters, instead of the T7 promoter. Queens initially investigated the differences in protein production between T7 and constitutive promoters, using available Biobricks in the iGEM registry. We helped them find the BioBricks for testing and helped in developing a testing method for measuring the protein production of said BioBricks.
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<h1 class="sub1">Collaboration 3 - iGEM Alberta Equality Series on Instagram</h1>
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<p class="para1">As a collaboration with iGEM Alberta, Nicole took part in their Equality Series on Instagram. This series highlights women and minorities in the sciences, with a goal to empower women in iGEM and in Science by showing how positive this experience is.
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<h1 class="sub1">Collaboration 4 - oGEM Meetup at Western University</h1>
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<p class="para1">Earlier in the competition year we collaborated with the Ontario iGEM teams to meet and discuss the progress in our projects. We presented on our progress and received feedback from other iGEM teams in what direction to take our project. We also provided feedback to other teams on their projects and provided advice on how to incorporate PIs into the team better as we have very good incorporation of our PIs.
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Revision as of 22:39, 15 October 2019




Collaborations

Collaboration 1 - Ontario iGEM Meet-Up

As a collaboration with all other Ontario iGEM teams, we hosted a meetup at the University of Guelph. iGEM Teams presented their project, and were judged and given feedback in order to prepare for the Giant Jamboree. At this meetup, there was a discussion with Ontario Genomics about the sustainability of iGEM teams and how to ensure students can continue to take part in synthetic biology and iGEM with the high price of the competition. This was a great opportunity for all iGEMers from Ontario to voice their opinion and share how their team operates.

Collaboration 2 - Queens iGEM Project Trouble-Shooting and Part Characterization

iGEM Guelph's Nicole assisted Queens iGEM with troubleshooting their protein expression. Recombinant antibody expression in E. coli is notoriously difficult and Queens was unable to express their BioBrick in BL21 cells over an extended period of time. Nicole made suggestions be able to better express their antibodies including: altering buffer conditions, changing expression conditions, altering the purification system, and trying a different cell line. This ultimately lead to Queens iGEM changing cell lines since their protein was more likely to fold in a cell line that contains an oxidizing cytoplasmic environment.

Additionally, we helped Queens iGEM develop their Bronze Medal characterization experiments for parts BBa_K608010 and BBa_I746909. One of the suggestions we made for improving their protein yield was trying constitutive promoters, instead of the T7 promoter. Queens initially investigated the differences in protein production between T7 and constitutive promoters, using available Biobricks in the iGEM registry. We helped them find the BioBricks for testing and helped in developing a testing method for measuring the protein production of said BioBricks.

Collaboration 3 - iGEM Alberta Equality Series on Instagram

As a collaboration with iGEM Alberta, Nicole took part in their Equality Series on Instagram. This series highlights women and minorities in the sciences, with a goal to empower women in iGEM and in Science by showing how positive this experience is.

Collaboration 4 - oGEM Meetup at Western University

Earlier in the competition year we collaborated with the Ontario iGEM teams to meet and discuss the progress in our projects. We presented on our progress and received feedback from other iGEM teams in what direction to take our project. We also provided feedback to other teams on their projects and provided advice on how to incorporate PIs into the team better as we have very good incorporation of our PIs.

Thank you to all of our iGEM collaborators,


we are glad to have been able to work together!

University of Guelph iGEM 2019





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Collaborations

Sharing and collaboration are core values of iGEM. We encourage you to reach out and work with other teams on difficult problems that you can more easily solve together.

Silver Medal Criterion #2

Document your collaboration(s) clearly on this page to compete for the silver medal criterion #2 on collaboration. Please see the 2019 Medals Page for more information.

Which other teams can we work with?

You can work with any other team in the competition, including standard, software, open, and high school track teams. You can also work with non-iGEM research groups, but they do not count towards the iGEM team collaboration silver medal criterion.

In order to meet the silver medal criteria on helping another team, you must complete this page and detail the nature of your collaboration with another iGEM team.

Here are some suggestions for projects you could work on with other teams:

  • Improve the function of another team's BioBrick Part or Device
  • Characterize another team's part
  • Debug a construct
  • Model or simulate another team's system
  • Test another team's software
  • Help build and test another team's hardware project
  • Mentor a high-school team