Difference between revisions of "Team:Marburg"
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Revision as of 19:17, 14 October 2019
Fastest.
Phototrophic.
Organism.
Establishing the fastest growing phototrophic organism with a doubling time of 90 min as a chassis for synthetic biology and the iGEM community.
We created an easy to use toolbox for Synechococcus elongatus UTEX 2973 to empower rapid design testing, including genome engineering tools, self-replicating plasmid systems, natural competence and a Golden Gate-based part library. By providing our fast phototrophic chassis to the community, we are paving the way for other phototrophic organisms in synthetic biology.
Strain engineering
We created an “easy to use” phototrophic chassis by restoring the natural competence of S. elongatus UTEX 2973 in order to enormously simplify the transformation process. We established a genome modification system via the CRISPR/Cpf1 and enabled the usage of self-replicating plasmids overcoming the drawbacks of time intensive genome integration for genetic design testing.
The Toolbox
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Drylab
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Measurement
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Metabolic Engineering
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