Difference between revisions of "Team:Marburg"

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             <h1 style="font-size: 1.5em;">Strain engineering</h1>
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               With the help of our MoClo compatible CRISPR/cpf1 system we were able to restore the natural
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               To create an “easy to use” phototrophic chassis we restored the natural competence of S. elongatus UTEX 2973,
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              competence of S. elongatus UTEX 2973. Curing it of its endogenous plasmid enabled us to use its origin of replication for
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              established a genome engineering system and enabled the usage of self-replicating plasmids overcoming the drawbacks of
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              self-replicating plasmids.
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              time intensive genome integration.
 
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Revision as of 17:23, 13 October 2019

Fastest.

Phototrophic.

Organism.

Establishing the fastest growing phototrophic organism with a doubling time of 90 min as a chassis for synthetic biology and the iGEM community.

We created an easy to use toolbox for Synechococcus elongatus UTEX 2973 to empower rapid design testing, including genome engineering tools, self-replicating plasmid systems, natural competence and a Golden Gate-based part library. By providing our fast phototrophic chassis to the community, we are paving the way for other phototrophic organisms in synthetic biology.

Strain engineering

To create an “easy to use” phototrophic chassis we restored the natural competence of S. elongatus UTEX 2973, established a genome engineering system and enabled the usage of self-replicating plasmids overcoming the drawbacks of time intensive genome integration.

The Toolbox

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Drylab

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Measurement

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Metabolic Engineering

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