Line 1: | Line 1: | ||
− | + | {{Fudan-TSI}} | |
− | + | <html> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | } | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
Line 501: | Line 436: | ||
− | |||
/*highlight */ | /*highlight */ | ||
Line 610: | Line 544: | ||
<meta name="viewport" content="width=device-width, initial-scale=1"> | <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
− | <script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/jQuery& | + | <script type="text/javascript" src="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/jQuery&action=raw&ctype=text/javascript"></script> |
Line 616: | Line 550: | ||
</head> | </head> | ||
− | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css& | + | <link rel="stylesheet" href="https://2019.igem.org/wiki/index.php?title=Template:Fudan-TSI/Fudan-font-awesome.css&action=raw&ctype=text/css" /> |
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | ||
Line 622: | Line 556: | ||
<!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | <!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | ||
+ | |||
+ | <style> | ||
+ | |||
+ | *{margin: 0;padding: 0;list-style: none;} | ||
+ | /* via: https://blog.csdn.net/weixin_41014370/article/details/79523637 */ | ||
+ | |||
+ | /** 清除内外边距 **/ | ||
+ | body, h1, h3, h3, h4, h5, h6, hr, p, blockquote, /* structural elements 结构元素 */ | ||
+ | dl, dt, dd, ul, ol, li, /* list elements 列表元素 */ | ||
+ | pre, /* text formatting elements 文本格式元素 */ | ||
+ | form, fieldset, legend, button, input, textarea, /* form elements 表单元素 */ | ||
+ | th, td /* table elements 表格元素 */ { | ||
+ | margin: 0; | ||
+ | padding: 0; | ||
+ | } | ||
+ | |||
+ | /** 设置默认字体 **/ | ||
+ | |||
+ | /* @@@@ h1, h3, h3, h4, h5, h6 { font-size: 100%; }*/ | ||
+ | address, cite, dfn, em, var { font-style: normal; } /* 将斜体扶正 */ | ||
+ | code, kbd, pre, samp { font-family: courier new, courier, monospace; } /* 统一等宽字体 */ | ||
+ | /* @@@@ small { font-size: 12px; } /* 小于 12px 的中文很难阅读,让 small 正常化 */ | ||
+ | |||
+ | /** 重置列表元素 **/ | ||
+ | ul, ol { list-style: none; } | ||
+ | |||
+ | /** 重置文本格式元素 **/ | ||
+ | a { text-decoration: none; } | ||
+ | a:hover { text-decoration: underline; } | ||
+ | |||
+ | |||
+ | /** 重置表单元素 **/ | ||
+ | legend { color: #000; } /* for ie6 */ | ||
+ | fieldset, img { border: 0; } /* img 搭车:让链接里的 img 无边框 */ | ||
+ | button, input, select, textarea { font-size: 100%; } /* 使得表单元素在 ie 下能继承字体大小 */ | ||
+ | /* 注:optgroup 无法扶正 */ | ||
+ | |||
+ | /** 重置表格元素 **/ | ||
+ | table { border-collapse: collapse; border-spacing: 0; } | ||
+ | |||
+ | </style> | ||
+ | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Content begins ---------------------------------------------------------------------------------------------------------------------------------> | <!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Content begins ---------------------------------------------------------------------------------------------------------------------------------> | ||
<!----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | <!----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | ||
Line 1,519: | Line 1,489: | ||
<div class="row para1"> | <div class="row para1"> | ||
<div class="row"> | <div class="row"> | ||
− | <div class="col"> | + | <div class="col col-lg-12"> |
Our mutagenesis system uses the BL21 (DE3) <i>E. coli</i> strain transformed with two plasmids, a stringent plasmid named P<sub>target</sub> carrying the target sequence that we want to mutate, and a relaxed plasmid named P<sub>mutant</sub>, carrying the gene encoding the tools necessary for mutagenesis, i.e. reverse transcriptase (RT) and Cre. <br /><br /> | Our mutagenesis system uses the BL21 (DE3) <i>E. coli</i> strain transformed with two plasmids, a stringent plasmid named P<sub>target</sub> carrying the target sequence that we want to mutate, and a relaxed plasmid named P<sub>mutant</sub>, carrying the gene encoding the tools necessary for mutagenesis, i.e. reverse transcriptase (RT) and Cre. <br /><br /> | ||
As we are designing a brand-new mutagenesis system inside <i>E. coli</i>, we want to demonstrate whether and under what condition it can work, so we turn to modelling to answer these questions. Our modelling work is comprised of 3 parts. 1) We used 3 deterministic models to describe the 3 reaction steps of our system—induced expression, reverse transcription and recombination. This allows us to compute and maximize the yield of the recombined P<sub>target</sub> which in turn, contributes to the optimization of our experimental setup. 2) We simulated the recombination process stochastically and calculated the number of recombined products that occurred during one replication cycle of <i>E. coli</i>. 3) We combined the 3 reaction steps together using deterministic model and found that the two kinds of inducers can be added at the same time to achieve optimal recombination efficiency within one life-cycle of <i>E. coli</i>. | As we are designing a brand-new mutagenesis system inside <i>E. coli</i>, we want to demonstrate whether and under what condition it can work, so we turn to modelling to answer these questions. Our modelling work is comprised of 3 parts. 1) We used 3 deterministic models to describe the 3 reaction steps of our system—induced expression, reverse transcription and recombination. This allows us to compute and maximize the yield of the recombined P<sub>target</sub> which in turn, contributes to the optimization of our experimental setup. 2) We simulated the recombination process stochastically and calculated the number of recombined products that occurred during one replication cycle of <i>E. coli</i>. 3) We combined the 3 reaction steps together using deterministic model and found that the two kinds of inducers can be added at the same time to achieve optimal recombination efficiency within one life-cycle of <i>E. coli</i>. | ||
Line 1,910: | Line 1,880: | ||
− | + | ||
− | + | ||
<!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Content ends ---------------------------------------------------------------------------------------------------------------------------------> | <!------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Content ends ---------------------------------------------------------------------------------------------------------------------------------> | ||
<!----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | <!----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</html> | </html> |
Revision as of 12:30, 13 October 2019