Line 215: | Line 215: | ||
</div> | </div> | ||
<div style="width: 100%; padding: 2rem; display: flex; align-items: center; justify-content: center;"> | <div style="width: 100%; padding: 2rem; display: flex; align-items: center; justify-content: center;"> | ||
− | <p style="text-align: center; font-size: 1.5em;"> | + | <p style="text-align: center; font-size: 1.5em !important;"> |
Establishing the fastest growing phototrophic organism with a doubling time of 90 min as a chassis for synthetic biology | Establishing the fastest growing phototrophic organism with a doubling time of 90 min as a chassis for synthetic biology | ||
and the iGEM community. | and the iGEM community. | ||
Line 221: | Line 221: | ||
</div> | </div> | ||
<div style="width: 100%; padding: 2rem; display: flex; align-items: center; justify-content: center;"> | <div style="width: 100%; padding: 2rem; display: flex; align-items: center; justify-content: center;"> | ||
− | <p style="text-align: center; font-size: 1em;"> | + | <p style="text-align: center; font-size: 1em !important;"> |
We created an easy to use toolbox for Synechococcus elongatus UTEX 2973 to empower rapid design testing, including | We created an easy to use toolbox for Synechococcus elongatus UTEX 2973 to empower rapid design testing, including | ||
genome engineering tools, self-replicating plasmid systems, natural competence and a Golden Gate-based part library. By | genome engineering tools, self-replicating plasmid systems, natural competence and a Golden Gate-based part library. By | ||
Line 238: | Line 238: | ||
<div class="text1"> | <div class="text1"> | ||
<h1 style="font-size: 1.5em;">Strain engineering</h1> | <h1 style="font-size: 1.5em;">Strain engineering</h1> | ||
− | <p style="font-size: 1em;"> | + | <p style="font-size: 1em; !important"> |
With the help of our MoClo compatible CRISPR/cpf1 system we were able to restore the natural | With the help of our MoClo compatible CRISPR/cpf1 system we were able to restore the natural | ||
competence of S. elongatus UTEX 2973. Curing it of its endogenous plasmid enabled us to use its origin of replication for | competence of S. elongatus UTEX 2973. Curing it of its endogenous plasmid enabled us to use its origin of replication for | ||
Line 250: | Line 250: | ||
<div> | <div> | ||
<h1 style="font-size: 1.5em;">The Toolbox</h1> | <h1 style="font-size: 1.5em;">The Toolbox</h1> | ||
− | <p style="font-size: 1em;"> | + | <p style="font-size: 1em; !important"> |
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | ||
dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip | dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip | ||
Line 262: | Line 262: | ||
<div> | <div> | ||
<h1 style="font-size: 1.5em;">Drylab</h1> | <h1 style="font-size: 1.5em;">Drylab</h1> | ||
− | <p style="font-size: 1em;"> | + | <p style="font-size: 1em; !important"> |
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | ||
dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip | dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip | ||
Line 274: | Line 274: | ||
<div> | <div> | ||
<h1 style="font-size: 1.5em;">Measurement</h1> | <h1 style="font-size: 1.5em;">Measurement</h1> | ||
− | <p style="font-size: 1em;"> | + | <p style="font-size: 1em; !important"> |
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | ||
dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip | dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip | ||
Line 286: | Line 286: | ||
<div> | <div> | ||
<h1 style="font-size: 1.5em;">Metabolic Engineering</h1> | <h1 style="font-size: 1.5em;">Metabolic Engineering</h1> | ||
− | <p style="font-size: 1em;"> | + | <p style="font-size: 1em; !important"> |
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | ||
dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip | dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip |
Revision as of 10:24, 5 October 2019
Fastest.
Phototrophic.
Organism.
Establishing the fastest growing phototrophic organism with a doubling time of 90 min as a chassis for synthetic biology and the iGEM community.
We created an easy to use toolbox for Synechococcus elongatus UTEX 2973 to empower rapid design testing, including genome engineering tools, self-replicating plasmid systems, natural competence and a Golden Gate-based part library. By providing our fast phototrophic chassis to the community, we are paving the way for other phototrophic organisms in synthetic biology.
Strain engineering
With the help of our MoClo compatible CRISPR/cpf1 system we were able to restore the natural competence of S. elongatus UTEX 2973. Curing it of its endogenous plasmid enabled us to use its origin of replication for self-replicating plasmids.
The Toolbox
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Drylab
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Measurement
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Metabolic Engineering
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.