Difference between revisions of "Team:Marburg"
| Line 218: | Line 218: | ||
With the help of our MoClo compatible CRISPR/cpf1 system we were able to restore the natural | With the help of our MoClo compatible CRISPR/cpf1 system we were able to restore the natural | ||
competence of S. elongatus UTEX 2973. Curing it of its endogenous plasmid enabled us to use its origin of replication for | competence of S. elongatus UTEX 2973. Curing it of its endogenous plasmid enabled us to use its origin of replication for | ||
| − | self-replicating | + | self-replicating plasmids. |
| − | + | ||
</p> | </p> | ||
</div> | </div> | ||
| Line 229: | Line 228: | ||
<h1 style="font-size: 1.5em;">The Toolbox</h1> | <h1 style="font-size: 1.5em;">The Toolbox</h1> | ||
<p style="font-size: 1em;"> | <p style="font-size: 1em;"> | ||
| + | Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | ||
| + | dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip | ||
| + | ex ea commodo consequat. | ||
</p> | </p> | ||
</div> | </div> | ||
| Line 238: | Line 240: | ||
<h1 style="font-size: 1.5em;">Drylab</h1> | <h1 style="font-size: 1.5em;">Drylab</h1> | ||
<p style="font-size: 1em;"> | <p style="font-size: 1em;"> | ||
| + | Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | ||
| + | dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip | ||
| + | ex ea commodo consequat. | ||
</p> | </p> | ||
</div> | </div> | ||
| Line 247: | Line 252: | ||
<h1 style="font-size: 1.5em;">Measurement</h1> | <h1 style="font-size: 1.5em;">Measurement</h1> | ||
<p style="font-size: 1em;"> | <p style="font-size: 1em;"> | ||
| + | Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | ||
| + | dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip | ||
| + | ex ea commodo consequat. | ||
</p> | </p> | ||
</div> | </div> | ||
| Line 256: | Line 264: | ||
<h1 style="font-size: 1.5em;">Metabolic Engineering</h1> | <h1 style="font-size: 1.5em;">Metabolic Engineering</h1> | ||
<p style="font-size: 1em;"> | <p style="font-size: 1em;"> | ||
| + | Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et | ||
| + | dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip | ||
| + | ex ea commodo consequat. | ||
</p> | </p> | ||
</div> | </div> | ||
Revision as of 07:32, 11 September 2019
Fastest.
Phototrophic.
Organism.
Establishing the fastest growing phototrophic organism with a doubling time of 90 min as a chassis for synthetic biology and the iGEM community.
We created an easy to use toolbox for Synechococcus elongatus UTEX 2973 to empower rapid design testing, including genome engineering tools, self-replicating plasmid systems, natural competence and a Golden Gate-based part library. By providing our fast phototrophic chassis to the community, we are paving the way for other phototrophic organisms in synthetic biology.
Strain engineering
With the help of our MoClo compatible CRISPR/cpf1 system we were able to restore the natural competence of S. elongatus UTEX 2973. Curing it of its endogenous plasmid enabled us to use its origin of replication for self-replicating plasmids.
The Toolbox
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Drylab
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Measurement
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.
Metabolic Engineering
Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.