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Revision as of 09:31, 8 September 2019
Fastest.
Phototrophic.
Organism.
Establishing the fastest growing phototrophic organism with a doubling time of 90 min as a chassis for synthetic biology and the iGEM community.
We created an easy to use toolbox for Synechococcus elongatus UTEX 2973 to empower rapid design testing, including genome engineering tools, self-replicating plasmid systems, natural competence and a Golden Gate-based part library. By providing our fast phototrophic chassis to the community, we are paving the way for other phototrophic organisms in synthetic biology.
Strain engineering
With the help of our MoClo compatible CRISPR/cpf1 system we were able to restore the natural competence of S. elongatus UTEX 2973. Curing it of its endogenous plasmid enabled us to use its origin of replication for self-replicating plasmids.