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| When working in Synthetic Biology, reporter genes such as fluorescence proteins are indispensable elements to characterize BioBricks. For a good characterization a suitable reporter is required. But reporters can be more than just merely a detection tool for transcriptional activity but they can also give a deeper insight into cellular conditions beyond the genetic context. We provide a <a href="https://2019.igem.org/Team:Marburg/Results#marburg_collection" target="_blank">diverse set of reporters</a> not only for the purpose of <a href="https://2019.igem.org/Team:Marburg/Improve">describing genetic tools</a> but also for the sensing of a variety of parameters which are crucial for cyanobacteria. | | When working in Synthetic Biology, reporter genes such as fluorescence proteins are indispensable elements to characterize BioBricks. For a good characterization a suitable reporter is required. But reporters can be more than just merely a detection tool for transcriptional activity but they can also give a deeper insight into cellular conditions beyond the genetic context. We provide a <a href="https://2019.igem.org/Team:Marburg/Results#marburg_collection" target="_blank">diverse set of reporters</a> not only for the purpose of <a href="https://2019.igem.org/Team:Marburg/Improve">describing genetic tools</a> but also for the sensing of a variety of parameters which are crucial for cyanobacteria. |
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| + | As an addition to the fluorescent reporters, we included a set of luminescent reporters to bring measurements of genetic constructs to a new level as they mostly bypass autofluorescence from cyanobacterial cells. The red-shifted version of NanoLuc -namely teLuc- bears the potential of the best reporter in <i>S. elongatus</i> as its absorbance bypasses autofluorescent signals better than NanoLuc and shows a higher relative bioluminescent signal<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5678970/" target="_blank">(Yeh <i>et al.</i>, 2017)</a>. |
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